Tiwari et al
Greener Journal of Agricultural Sciences Vol. 3 (12), pp. 836-842, December 2013.
ISSN: 2276-7770 © 2011 Greener Journals
Manuscript Number: 111413963
An Uncultured Bacterium Associated with Infection in Capsicum annuum in India
Richa Tiwari*1, Sudhanshu Tiwari2, P.P. Upadhyaya1
1Plant Pathology Lab, Department of Botany, D.D.U. Gorakhpur University Gorakhpur-273009, U.P., India.
2J.H.S. Rajdhani, Gorakhpur, U.P., India.
*Corresponding Author’s Email: richa19832013 @ gmail.com
We make an extensive survey of Gorakhpur district of Eastern
U.P. region of India from 2011-2013, for vegetable plant
samples showing visible symptoms of possible phytoplasmal
infection. We observed symptoms such as reduction in leaf
size, curling of leaf, etc. in infected chilli plants (Capsicum
annuum L., Family Solanaceae), growing in the
fields of Gorakhpur district. By using universal phytoplasma
specific P1 and Tint primers, we perform their PCR analysis.
The PCR product of size 1600 bp was generated which were
further purified and sequenced. Nucleotide sequence obtained
in present study was deposited in GenBank through accession
number KF298062 and named as “Uncultured Pseudomonas sp.'
with/ clone=RCGKP1”. BLAST analysis and Phylogenetic tree
established their association with ‘Pseudomonas’ group. In
our knowledge, this is a new uncultured bacterium (359 bp),
associated with infection in Capsicum annuum plant in
Gorakhpur district of Eastern U.P. region of India.
Keywords: Capsicum annuum, Uncultured Pseudomonas sp.' with/clone=RCGKP1, Accession number KF298062.