Greener Journal of Agricultural Sciences

Open Access

Tiwari et al

Greener Journal of  Agricultural Sciences Vol. 3 (12), pp. 836-842, December 2013.

 ISSN: 2276-7770 © 2011 Greener Journals

Research Paper

Manuscript Number: 111413963

 

An Uncultured Bacterium Associated with Infection in Capsicum annuum in India

 

Richa Tiwari*1, Sudhanshu Tiwari2, P.P. Upadhyaya1

 

1Plant Pathology Lab, Department of Botany, D.D.U. Gorakhpur University Gorakhpur-273009, U.P., India.

 2J.H.S. Rajdhani, Gorakhpur, U.P., India.

 

*Corresponding Author’s Email: richa19832013 @ gmail.com


Abstract:

We make an extensive survey of Gorakhpur district of Eastern U.P. region of India from 2011-2013, for vegetable plant samples showing visible symptoms of possible phytoplasmal infection. We observed symptoms such as reduction in leaf size, curling of leaf, etc. in infected chilli plants (Capsicum annuum L., Family Solanaceae), growing in the fields of Gorakhpur district. By using universal phytoplasma specific P1 and Tint primers, we perform their PCR analysis. The PCR product of size 1600 bp was generated which were further purified and sequenced. Nucleotide sequence obtained in present study was deposited in GenBank through accession number KF298062 and named as “Uncultured Pseudomonas sp.' with/ clone=RCGKP1”. BLAST analysis and Phylogenetic tree established their association with ‘Pseudomonas’ group. In our knowledge, this is a new uncultured bacterium (359 bp), associated with infection in Capsicum annuum plant in Gorakhpur district of Eastern U.P. region of India.
 
Keywords: Capsicum annuum, Uncultured Pseudomonas sp.' with/clone=RCGKP1, Accession number KF298062.

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