Greener Journal of Agricultural Sciences

Excellence and Timeliness

  
  

Change Language

Tiwari et al

Greener Journal of  Agricultural Sciences Vol. 3 (12), pp. 836-842, December 2013.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 111413963

 

An Uncultured Bacterium Associated with Infection in Capsicum annuum in India

 

Richa Tiwari*1, Sudhanshu Tiwari2, P.P. Upadhyaya1

 

1Plant Pathology Lab, Department of Botany, D.D.U. Gorakhpur University Gorakhpur-273009, U.P., India.

 2J.H.S. Rajdhani, Gorakhpur, U.P., India.

 

*Corresponding Author’s Email: richa19832013 @ gmail.com


Abstract:

We make an extensive survey of Gorakhpur district of Eastern U.P. region of India from 2011-2013, for vegetable plant samples showing visible symptoms of possible phytoplasmal infection. We observed symptoms such as reduction in leaf size, curling of leaf, etc. in infected chilli plants (Capsicum annuum L., Family Solanaceae), growing in the fields of Gorakhpur district. By using universal phytoplasma specific P1 and Tint primers, we perform their PCR analysis. The PCR product of size 1600 bp was generated which were further purified and sequenced. Nucleotide sequence obtained in present study was deposited in GenBank through accession number KF298062 and named as “Uncultured Pseudomonas sp.' with/ clone=RCGKP1”. BLAST analysis and Phylogenetic tree established their association with ‘Pseudomonas’ group. In our knowledge, this is a new uncultured bacterium (359 bp), associated with infection in Capsicum annuum plant in Gorakhpur district of Eastern U.P. region of India.
 
Keywords: Capsicum annuum, Uncultured Pseudomonas sp.' with/clone=RCGKP1, Accession number KF298062.

Return to Content   View Reprint (PDF) (804KB)  


Call for Articles/Books

Call for Scholarly Articles


Authors from around the world are invited to send scholary articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles daily, immediately they are ready.


The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.


Use our quick submit button to submit or simply send your article(s) as an e-mail attachment to manuscripts@acad.gjournals.org or manuscripts.igj@gmail.com.


Call for Books


You are also invited to submit your books for online or print publication. We publish books related to all academic subject areas.    Submit as an e-mail attachment to books@acad.gjournals.org.




Search

Login Form

Other Journals


Newsletters


Sponsored