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Konate et al

Greener Journal of Agricultural Sciences Vol. 5 (1), pp. 043-051, February 2015.

 ISSN: 2276-7770  

Research Paper

Manuscript Number: 121114393

DOI: http://doi.org/10.15580/GJAS.2015.1.121114393


Screening of Endophytic Bacteria Associated with Ceratonia siliqua L. Plant Using Molecular Marker Repetitive Extragenic Palindromic (Rep)-PCR

 

Ibrahim Konate1,*, Mathurin Koffi1, Annick Koulibaly1, Amina Sorouri2, El Bekkay Berraho2, Abdelkarim Filali-Maltouf2

 

1Unité de Formation et de Recherche en Agroforesterie et en Environnement, Laboratoire des Interactions Hôte-Microorganisme, Environnement et Evolution (LIHME), Université Jean Lorougnon Guédé, B.P: 150 Daloa, Côte d’Ivoire.

2Faculty of Sciences, Laboratory of Microbiology and Molecular Biology, University Mohammed V-Agdal, Rabat, Morocco.

 

*Corresponding author’s email: konatibrahim@ yahoo.fr, BP: 150 Daloa (Côte d’Ivoire),

Fax: (+225) 32 78 75 70


Abstract

 

Repetitive extragenic palindromic (Rep)-PCR was used to screen and to characterize the natives endophytic bacteria isolated from roots (IRC) and epicotyls (IEC) of young carob (Ceratonia siliqua L.) plant collected from different Moroccan localities. Genomic DNA of 80 bacteria (69 IRC and 11 IEC), 7 strains of RCM and 13 strains of reference rhizobia were successfully carried. The complementary primers of Rep induced reproducible PCR fingerprint patterns and generated more bands polymorphs and useful for distinguishing the isolates from each other. We obtained respectively 6, 17 and 35 polymorphic bands with IEC, RCM and IRC strains with the size ranging between 380 to 7250 pb. Dendrogram based upon UPGMA analysis of Rep-PCR patterns showed high degree of genetic diversity among indigenous carob endophytic bacteria. At 87 % of similarity, we revealed a wide polymorphism and obtained 2 greats clusters, 6 smalls groups and 24 independent lines. Rep-PCR technology allowed us, firstly to characterize 14 IRC genetically belonging to RMC, Sinorhizobium sp, Rhizobium sp and Agrobacterium sp; and secondly, to revalue the exact number of our collection to 45 strains and to select excessively34 endophytic bacteria original from seven Moroccan regions for futures works.     

 

Key words: Enophytic bacteria, Carob (Ceratonia siliqua L), REP-PCR, screen, genetic diversity.


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Cite this Article: Konate I, Koffi M, Koulibaly A, Sorouri A, Berraho EB, Filali-Maltouf A, 2015. Screening of Endophytic Bacteria Associated with Ceratonia siliqua L. Plant Using Molecular Marker Repetitive Extragenic Palindromic (Rep)-PCR. Greener Journal of Agricultural Sciences, 5(1):043-051, http://doi.org/10.15580/GJAS.2015.1.121114393.


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