Greener Journal of Agricultural Sciences Vol. 3 (6), pp. 492-496, June 2013.
ISSN: 2276-7770 © 2011 Greener Journals
Manuscript Number: 011613389
Screening of Some Fungi Associated with Maize Cob Degradation for Cellulase Activity
Bamigboye, O. O.
Department of Biology, Emmanuel Alayande College of Education, Oyo.
Email: bamigboyetaitoy @ yahoo. com
Fungi involved in the biodegradation of maize cob were isolated and screened for cellulase activity using glucose and carboxylmethyl cellulose as carbon sources. The effect of the carbon sources on cellulase production was determined.
Eleven fungi species were isolated which include Rhizopus
oryzae, Aspergillus flavus
Mucor racemosus, Aspergillus niger, Penicillium atrovenetum,
Penicillium expansum, Botryotrichum piluliferum, Penicillium
chrysogenum and Penicillium restictum. Effect of
carbon source on cellulase production was also reported. The
highest reducing sugar production for all fungi isolate was
obtained on day 14 when carboxymethyl cellulose (CMC) was
used as the carbon source; while the highest reducing sugar
production was obtained on day 7 when glucose was used as
the carbon source
Aspergillus niger had the highest production of
reducing sugar (1.68mg/ml and 0.82/m) in CMC and glucose
respectively while the lowest production was recorded for
Mucor racemosus (0.69mg/,ml and 0.01mg/ml). All
Aspergillus species and Penicilluim
species isolated had very high reducing sugar production in
Aspergillus flavus 1.24 mg/ml; A oryzae
1.08mg/ml, P expansum ,
1.60 mg/ml, P atrovenetum 1.43mg/ml) except
Penicillium chrysonenum and Penicillium restrictum
with 0.78mg/ml and 0.81 mg/ml, respectively.
Aspergillus and Penicillum species are good cellulase producers especially those that are associated with degrading maize cob. These organisms are considered suitable to increase the nutrient of maize cob, an agro industrial waste and consequently make them useful for animal feed production.
Keywords: Cellulase, Fungi, Reducing Sugar, Maize-cob, Biodegradation.