Greener Journal of Agricultural Sciences

Open Access

Yousr et al

Greener Journal of  Agricultural Sciences Vol. 4 (5), pp. 178-186, June 2014.

 ISSN: 2276-7770 © 2011 Greener Journals

Research Paper

Manuscript Number: 021014100


Effects of Controlled Mycorrhization on Production of Jacaranda mimosifolia D.Don


Zaouchi Yousr*, Ben Bahri Nada, Ben Jeddi Faysal, Rezgui Salah, Bettaieb Taoufik


Institut National Agronomique de Tunisie (INAT), 1082 Tunis - Mahrajène, Tunisie.


*Corresponding Author’s Email:, Tel: (+216) 71 28 71 10,

Fax: (+216) 71 79 93 91


Growth and mineral absorption of ornamental woody plant such as Jacaranda mimosifolia D. Don., in gnotoxenic conditions out of a mycorrhization by a commercial inoculum containing Glomus irregulare were studied.

Used Jacaranda plants are one- year old, they were first grown, in 10/12cm caliber pots then in 16/18cm caliber pots for 6 months in a sterile substrate consisting of 2/3 commercial peat and 1/3 sand. Four treatments, achieved three times, were processed. Apart from the control, two other treatments consisted of the addition of commercial inoculum containing Glomus irregulare to the culture substrate, at a rate of 62.5 mg / plant for the first and 100 mg / plant for the second. These quantities respectively correspond to 100 propagules / plant and 160 propagules / plant. The fourth treatment is illustrated by the incorporation of 4g/plant of OSMOCOT EXACT standard-Scotts fertilizer; 15 + 9 + 12 (+2.5), into the substrate.

Mycorrhization rate of inoculated plants by 100mg/plant dose reached 43.21% while it only reached 21.48% for those that received 62.5mg/plant. Colonization remains at the beginning of installation presenting essentially mycorrhizal hyphae structures.  Mycorrhization significantly improves nitrogen, phosphorus and potassium nutrition of Jacaranda. This results in a stimulation of plant growth Level of mycorrhization by 21.48% and achieve gains of aerial dry biomass of about 45.5% and root volume of about 46.29%. When the level of mycorrhization is equal to 43.21%, these gains correspond to, respectively, 59.1% and 67.28%. Fertilization provides for gains of 29.58% in aerial dry biomass and 9.69% in plants height. It has no effect on roots volume.

Keywords: Jacaranda mimosifolia D. Don- controlled mycorrhization- commercial inoculums- growth-nutrition.


Aliasgharzad N, Bolandnazar SA, Neyshabouri MR and Chaparzadeh N, (2009). Impact of soil sterilization and irrigation intervals on P and K acquisition by mycorrhizal onion (Allium cepa L.). Biologia 64: 512-515.

Aroca R, Porcel R and Ruíz-Lozano JM, (2007). How does arbuscular mycorrhizal symbiosis regulate root hydraulic properties and plasma membrane aquaporins in Phaseolus vulgaris under drought,cold or salinity stresses?. New Phytologist 173: 808–816.

Bolan NS, (1991). A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plant. Plant and soil 134 : 189-207.

Bousselmame F, Kenny  L and Achouri M, (2002). Effet des mycorhizes à vésicules et arbuscules sur la croissance et la nutrition de l’arganier (Argania spinosa L.). Revue Marocaine des Sciences Agronomiques et Vétérinaires. Actes Institut Agronomique et Vétérinaire (Maroc) 22 (4) : 193-198.

Bucher M, (2007). Functional biology of plant phosphate uptake at root and mycorrhiza interfaces. New Phytol. 173: 11-26.

Citernesi AS, Vitagliano C and Giovannetti M, (1998). Plant growth root system morphology of Olea europaea L. Rooted cuttings as influenced by arbuscular mycorrhizas. S J Horticult Sci Biotechnol. 73(5): 647-654

Clark RB and Zeto SK, (2000). Mineral acquisition by arbuscular mycorrhizal plants. Journal of Plant Nutrition 23: 867–902.

Dione Y, Sy MO, Diop AT, Diallo A and Ba AT, (2004). Réponse d’Allocasuarina verticillata (Lam.) L. Johnson et de Casuarina equisetifolia (L.) à l’inoculation mycorhizienne arbusculaire. J. Fac Sciences.  3 (1): 20-28.

Echairi A, Nouaim R and Chaussod R, (2008). Intérêt de la mycorhization contrôlée pour la production de plants d'arganier (Argania spinosa) en conditions de pépinière. Sécheresse 19 (4): 277-281.

Graham JH, (2000). Assessing costs of arbuscular mycorrhizal symbiasis in agroecosystems. In: Current advances in mycorrhizae research. Eds. Podila G.K., Douds D.D. Jr.: 127–140.

Heitholt  JJ, (1989). Water use efficiency and dry matter distribution in nitrogen- and water-stressed winter wheat.  Agronomy Journal 81: 464.

Hodge A, Campbell CD and Fitter AH, (2001). An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material. Nature 413: 297–299.

Ingleby K, Fahmer A, Wilson J, Newton AC, Manson PA and Smith RI, (2001). Interactions between mycorrhizal colonisation, nodulation and growth of Calliandra calothyrsus seedlings supplied with different concentrations of phosphorus solution. Symbiosis: 30 (1): 15-28.

Kjeldahl J, (1883). Neue Methode zur Bestimmung des Stickstoffs in organischen  Körpern. Z. Anal. Chem 22 : 366-382.

Laminou Manzo O, Ibrahim D, Campanella B and Paul R, (2009). Effets de l'inoculation mycorhizienne du substrat sur la croissance et la résistance au stress hydrique de  cinq espèces fixatrices de dunes : Acacia raddiana Savi ; Acacia nilotica (L.) Willd. Ex Del. var. adansonii ; Acacia senegal (L.) Willd ; Prosopis chilensis Stunz. et Bauhinia rufescens Lam.. Geo-Eco-Trop. 33,n.s. :  115 – 124.

Leye EHM, Ndiaye M, Ndiaye F, Diallo B, Sarr AS, Diouf M and Diop T, (2009). Effet de la mycorhization sur la croissance et le développement de Jatropha curcas L. Revue des Energies Renouvelables 12 (2) : 269 – 278.

Mäder PA, Vierheilig H, Streitwolf-Engel R, Boller T, Frey B, Christie P and Wiemken A, (2000). Transport of 15N from a soil compartment separated by a polytetrafluoroethylene membrane to plant roots via the hyphae of arbuscular mycorrhizal fungi. New phytologist 146: 155-161.

Marulanda A, Barea JM and Azcón R, (2006). An indigenous drought-tolerant strain of Glomus intraradices associated with a native bacterium improves water transport and root development in Retama sphaerocarpa. Microbiol Ecology 52: 670–678.

McGonigle TP and Fitter AH, (1990). Ecological specificity of vesicular-arbuscular mycorrhizal associations. Mycological Research 94 (1): 120–122.   

Miyajima I, Kato A, Hagiwara JC, Mata D, Facciuto G, Soto S, Escandon A, Mori M and Kobayashi N, (2005). Promotion of immature seed germination in Jacaranda mimosifolia. Hortscience 40: 1485-1486.

Morin F, Fortin JA, Hamel C, Granger RL and Smith DL, (1994). Apple rootstock response to vesicular arbuscular mycorrhizal fungi in a high phosphorus soil. J Amer Soc Hort Sci. 119(3): 578-583.

Moss B, Stribley DP and Le Tacon F, (1981). Ecology of mycorrhizae and mycorrhizal fungi. Advances Microbial Ecology 5: 134-210.

Musick GJ, Fairchild ML, Ferguson VL, Zuber MS and Crop J,  (1965). A method of measuring root volume in corn (Zea mays L.). Crop Science 5 : 601-602.

Nouaïm R and Chaussod R, (1996).  Rôle des mycorhizes dans l’alimentation hydrique des plantes, notamment des ligneux en zones arides. Options Méditerranéennes 20: 9-26.

Oihabi A, (1991). Étude des endomycorhizes à vésicules et arbuscules sur le Bayoud et la nutrition du palmier dattier. Thèse de doctorat :  Univ. Cadi Ayyad (Marrakech).

Olsen SR, Cole CV, Watanabe FS and Dean LA, (1954). Estimation of available phosphorus in soils by extraction with sodium bicarbonate.  USDA Circular, 939,1-19. Gov. Printing Office Washington D.C

Plenchette C and Fardeau JC, (1988). Effet du pouvoir fixateur du sol sur le prélèvement de phosphore du sol par les racines et les mycorhizes. C R Acad Sci Paris 306: 201-206.

Schubert A, Bodrino C and Gribaudo I, (1992). Vesicular-arbuscular mycorrhizal inoculation of kiwifruit (Actinidia deliciosa) micropropagated plants.  Agronomie 12: 847-850.

Sow HA, Diop TA, Ndiaye F, Manga AGB and Diallo A, (2008). Influence de la mycorhization arbusculaire sur la culture intensive de l'oignon (Allium cepa L.) au Sénégal. Journal des Sciences 8(1): 1-6.

Smith SS and Read DJ, (2008). Mycorrhizal Symbiosis. Academic Press.

Strullu DG and Plenchette C, (1991). Les mycorhizes en horticulture. PHM Revue Horticole 352 : 50-55. 

Tellal M, Qarro M, Arahou M, Abourouh M, Tellal R, Rhazi L and Douira A, (2008). Effet de l’actinomycète Frankia sur la croissance et la fixation de l’azote de Casuarina glauca et Casuarina cunninghamiana. Science et changements planétaires / Sécheresse 19 (3) : 211-6.

Trépanier M, (1998). Effet des champignons endomycorhiziens sur le bouturage et la croissance de plantes ligneuses ornementales. Thèse de doctorat : Faculté des sciences de l’agriculture et de l’alimentation- Département de phytologie - Université de Laval (France).