Greener Journal of Agricultural Sciences

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Greener Journal of Agricultural Sciences Vol. 8 (6), pp. 110-118, June 2018.

ISSN: 2276-7770  

Research Article

Manuscript Number: 051518071




Evaluation of Grevillae Robusta Dry Leaves Supplemented with Cotton Seed Waste for the Cultivation of Oyster mushroom (Pleurotusostreatus)



ASEFA Keneni


 Department of Biology, Ambo University, Ambo, P.O.Box.19, Ethiopia.



At present more emphasis has been given to mushroom production for the nutritionand, medicinaluses and waste recycling technology. The main aim of the present study was to report the usability of Greviillaerobusta dry foliage as a major substrate for production of oyster (Pleurotusostreatus) mushroom with supplement of different proportion of cotton seed waste. The culture of the oyster mushroom was maintained on potato dextrose agar, and the spawn was prepared on yellow colored sorghum and sterilized substrate was inoculated with 5% of the spawn wetbasis on dry basis of the substrate.The experimental design constitutes nine treatments (T1-T9) in three replicates from the middle of November2017 to the end of February 2018. Fastest mycelia run was observed in the treatments T9, T7 and T8: 10 days each from inoculation, while the slowest mycelia runwas observed in treatment T5 and T6: 19 days each from inoculation. Longest production cycle wasobserved for treatment T3, T5 and T6: 111days each, while the shortest production cycle was recordedfor treatment 8: 100days. Highest fresh weight1246.5 g /600g dry substrate was recorded for T 9; highest number (130)of fruits recorded for T4 while largest cap diameter(8cm) was recorded for treatment T6.The T1 showed the  lowest fresh weight, 538g/600 g/dry weight of the substrate, with  70 fruits and 6.5cm cap diameter.No Significantdifference were observed for the stipe length of the different treatments. Highest biological efficiency was recorded for T9: 208; and 89.5 for T1.Even though the cotton seed waste proportion supplementation varied for the treatments all except T1 gave more than 100% biological efficiency which makes Grevillae dry leaves, a good substratum for mass production of oyster mushroom which can produce good quality mushroom fruit bodies.


Keywords: Cotton seed waste, growth, Grevlliae dry leave, oyster mushroom, yield.

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