Madakbaş et al
Greener Journal of Agricultural Sciences Vol. 4 (2), pp. 039-045, March 2014.
Manuscript Number: 021014101
Determination of PhaseolinTypes
Seher Yıldız Madakbaş1*, Halima Awale2 and
1Ahi Evran University, Agricultural Faculty, Department of Horticulture, Kırşehir-Turkey.
2Michigan State University, Department of Crop, Soil and Microbial Sciences, Michigan- USA.
*Corresponding Author’s Email: seheryldzmadakbas @ gmail.com
The purpose of
our study was to determine the phaseolin types of common
bean varieties widely consumed in Turkey through protein
extraction. Twenty- four bean genotypes, consisting of 22
varieties from Kırsehir province of Turkey and two control
varieties (Andean and Mesoamerican) were assessed. Sodium
dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
was performed with protein samples extracted from bean
genotypes. Gels were stained with Coomassie blue dye R-250
and kept in fixing solution. Our analyses revealed that out
of 22 bean samples, 16 contained Andean phaseolin type and 6
contained Mesoamerican phaseolin type. T-test statistical
analysis was performed to determine the relationship between
seed phaseolin types and seed volumes (length, height and
width). The results showed that there was a significant
difference between phaseolin and seed volume.
Keywords: Phaseolus vulgaris, phaseolin, SDS-PAGE, genotype, Andean, Mesoamerican, seed volume.
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