Greener Journal of Agricultural Sciences

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Freitas et al

Greener Journal of Agricultural Sciences Vol. 6 (3), pp. 087-092, March 2016.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 063014287



Inoculation of Bradyrhizobium japonicum (Kirchner, 1896) Jordan (1982) (Bradyrhizobiaceae) on Inga edulis Mart. (Leguminosae, Mimosoideae)


Freitas J*1, Mônico AZ1, Lírio EJ2, Sarnaglia-Jnr VB2

Bonadeu F2Silva JBNF 3 & Hebling SA4


1Centro Universitário Norte do Espírito Santo – CEUNES/UFES. Rodovia BR 101 Norte, km. 60, Bairro Litorâneo, São Mateus – ES, Brazil, 29932-540.

2Escola Nacional de Botânica Tropical, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro. Rua Pacheco Leão, 915, sala 107, Jardim Botânico, Rio de Janeiro – RJ, Brazil, 22460-038.

3Centro de Ciências e Saúde (CCS), Universidade Federal do Rio de Janeiro. Av. Carlos Chagas Filho 373, Bloco J sala 25, Ilha do Fundão, Rio de Janeiro, RJ, Brazil, 22541-900.

4Faculdade Católica Salesiana do Espírito Santo. Av. Vitória, a950, Forte São João, Vitória ES, Brazil, 29017-950.


Legumes that have the ability to fix atmospheric nitrogen through symbiosis with bacteria known as rhizobia, have great importance from an economic and ecological perspective, because in agricultural crops and reforestation, may waive the use of nitrogenous fertilizers and provide more resistant seedlings, minimizing impacts in nature and low cost. Bradyrhizobium japonicum (Kirchner, 1896) Jordan (1982) (Bradyrhizobiaceae) is a rhizobium which has association with several species of the subfamily Papilionoideae. This study aimed to analyze the initial growth of Inga edulis Mart (Leguminosae, Mimosoideae) inoculated with B. japonicum to test interaction in the subfamily Mimosoideae. We used the parameters: height, stem diameter and number of expanded leaves; data analysis consisted of the Shapiro-Wilk normality test. The inoculated seedlings showed a significant difference for height and number of leaves, but no  significant difference for stem diameter. From the results, it is inferred that I. edulis presents positive interaction with B. japonicum, i.e., the interaction of rhizobia extends to Mimosoideae, which allows to infer that the interaction of rhizobia can be shown as homologous synapomorphy of the clade Mimosoideae-Papilonoideae, which is supported by molecular phylogenies. The results are compared with other studies and the implications in the evolution of the clades of the Leguminosae are discussed in phylogenetic studies. To corroborate the hypothesis, further studies of species of different subfamilies of these clades should be tested in order to verify that indeed this interaction is a homologous synapomorphy of the clade or an apomorphy for Mimosoideae, Leguminosae and interaction with B. japonicum has arisen more than once in the evolution scale of the family.


Keywords: Fabaceae, rhizobia, legume, agricultural crop.

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