Greener Journal of Agricultural Sciences

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Ndams et al

Greener Journal of Agricultural Sciences Vol. 6 (3), pp. 115-120, March 2016.

 ISSN: 2276-7770  

Research Paper

Manuscript Number: 022616045



Bioconversion of Methionine Supplemented Spent Sorghum Mash into Protein Biomassusing Rumen Liquor as Inoculum


Ndams SS, Jahun MB, Ibeh JI, Makeri HK and Ibrahim MM


College of Agriculture and Animal Science, Ahmadu Bello University, Mando Rd. Kaduna. Nigeria.


Four (4) samples of Spent Sorghum mash (SSM) with substrate to inoculums concentration of 10:1(w/w) were supplemented with 0.29, 0.33, 0.37, and 0.41% levels of methionine on dry matter(DM) basis. The same quantity of samples were compacted in four (4) jam bottles of equal volume to achieve anaerobic condition as much as possible and allowed to ferment for 4 days. The re-fermented spent sorghum mash (RSSM) samples were sun-dried for 3 days and subjected to analyses for proximate nutrient and amino acid compositions respectively.The DM% composition of SSM sample was significantly (P<0.05) higher than samples on 0.29, 0.33 and 0.39% methionine treatments. The crude Protein content of all samples on 0.29, 0.33, 0.39 and 0.41% methionine treatment did not differ significantly (P>0.05) but were significantly (P<0.05) higher than the control.  The CP% was observed to increase linearly with increase in the levels of methionine supplementation. The CF% composition of sample on control were similar to that on 0.29% methionine treatment but significantly (P<0.05) higher than that on 0.33, 0.37, and 0.41% methionine treatments. The RSSM sample showed the availability of all essential amino acids except tryptophan (alanine, arginine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine and valine). The results of this study indicates increase in quantity of protein and the availability of essential amino acids after second stage fermentation of spent sorghum mash with methionine supplementation and can therefore be  used in rations for non-ruminant animals in place of expensive conventional plant protein sources.


Keywords: Spent Sorghum Mash, Methionine, Inoculum, Fermentation.

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