Greener Journal of Agricultural Sciences

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Chennappa et al

Greener Journal of  Agricultural Sciences Vol. 4 (4), pp. 117-129, May 2014.

 ISSN: 2276-7770 

Research Paper

Manuscript Number: 010314003



Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils


 Chennappa G.1, Adkar-Purushothama C.R.2#Naik M.K.3

Suraj U.4, Sreenivasa M.Y.1*


1Department of Studies in Microbiology, University of Mysore, Manasagangotri,

Mysore- 570 006, Karnataka, India.

2Laboratory of Plant Pathology, Faculty of Agriculture and Life Science Hirosaki University, Bunkyo-cho 3, Hirosaki 036-8561, Aomori Ken, Japan #Present address: Pavilion for Applied Cancer Research, 

Department of Biochemistry

Faculty of Medicine and Health sciences, Sherbrooke University 3201, Rue Jean-Mignault, Sherbrooke, 

Quebec J1E 4K8, Canada.

3Department of Plant Pathology, College of Agriculture, University of Agricultural Sciences, Raichur – 584104, Karnataka, India.

4NRC on DNA finger printing, NBPGR, PUSA Campus, New Delhi - 110012 India.


1Email: chinnagurikar @ gmail. com


*Corresponding Author’s Email: sreenivasamy @ gmail. com, Fax: +91-821-2411208;

Telephone: +91-821-2419733


Azotobacter strains were isolated from paddy soils by serial dilution agar plate method.  The colonies were glistening; smooth, slimy, brown to black in morphology on Jenson’s N-free agar plates and the cells were Gram- negative bacteria. Biochemically, they were positive for indole production, citrate, catalase and Voges Proskauer test. Further, Nif gene sequence results revealed the presence of seven Azotobacter species. The effect of 1, 3 and 5% pesticides concentration viz., pendimethalin, chloropyrifos, glyphosate and phorate on nitrogen fixation, indole acetic acid, gibberllic acid, phosphate solubilization and bioassay of Azotobacter sp. were studied. Among all the species, GVT-1 strain was found to fix nitrogen at a maximum of 30µgN2 ml-1 day-1, produced highest quantity gibberllic acid (10µg25ml-1) and able to solubilize the phosphate at the rate of 9.8cm by forming the halo zone which was supplemented with 5% phorate. Similarly, GVT-1 strain produced a highest amount of indole acetic acid (31.8µgml-1) in 5% pendimethalin. Further bioassay activities of GVT-1 strain found efficient in increasing the root, shoot length and vigor of the plant. From these results it is clear that the Azotobacter sp. not only produces plant growth promoting substances but is also resistant to different pesticides and is not affected by the bacterial activity.

Keywords: Azotobacter; Nif gene; PGP activity; Bioassay; Pesticides.


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