Greener Journal of Agricultural Sciences

Open Access

Rocha et al

Greener Journal of  Agricultural Sciences Vol. 4 (4), pp. 110-116, May 2014.

 ISSN: 2276-7770 © 2011 Greener Journals

Research Paper

Manuscript Number: 012314055

DOI: http://dx.doi.org/10.15580/GJAS.2014.4.012314055

 

Introduction of the neMDH gene in Urochloa brizantha for Aluminium Tolerance in Agronomic Assays

 

Flavio Rocha1, Keila Maria Roncato Duarte*2,

Luiz Humberto Gomes1, Waldssimiller Teixeira Mattos3,

Simone Possedente Lira1, Paulo Bardauil Alcantara3

 

1Laboratório de Química Orgânica de Produtos Naturais, Departamento de Ciências Exatas, Escola Superior de Agricultura “Luiz de Queiroz”, USP, Piracicaba, 13418-900, São Paulo, Brazil.

2CPD Genetic and Animal Reproduction, Institute of Animal Science and Pastures, APTA/SAA Nova Odessa, 13460-000, São Paulo, Brazil.

3CPD Pastures and Nutrition , Institute of Animal Science and Pastures,

APTA/SAA Nova Odessa, 13460-000, São Paulo, Brazil.

 

*Corresponding Author’s Email: keiladuarte @ globo. com

Abstract:

Agricultural production in acid soils constituting about half of the total arable land on Earth, is limited by aluminium toxicity, which is manifested by root growth reduction, decreasing nutrients and water absorption by the plant. This work evaluated aluminium resistance in genotypes of Brachiaria brizantha cv. MG4, transformed with the neMDH gene, by using a nutrient solution for agronomic evaluation and gene integration confirmation by PCR. Three from the five confirmed transformed genotypes of B. brizantha cv. MG4 with the neMDH gene presented tolerance to aluminium solutions (111 µmol L -1 and 444 µmol L -1). Two from those three genotypes  also showed higher dry weight production in comparison to the wild-type genotype.

Keywords: Brachiaria, Tolerance, Aluminum, Soil, Transgenic, neMDH.

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