Salau et al
Greener Journal of Agricultural Sciences Vol. 6 (10), pp. 285-293, November 2016.
ISSN: 2276-7770 © 2015 Greener Journals
Manuscript Number: 092316144
Aflatoxin Contamination of Stored Groundnut Kernel in Sokoto State, Nigeria
*1Salau IA, 2Shehu K, 2Muhammad S and 3Umar RA
*1Department of Biology, Shehu Shagari College of Education, Sokoto Nigeria.
2Department of Biological Sciences, Usmanu Danfodiyo University, Sokoto Nigeria.
3Department of Biochemistry, Usmanu Danfodiyo University, Sokoto Nigeria.
Aflatoxins (AF) are a group of fungal toxins that contaminate foods and feeds with adverse health impact on human beings and animals. A total of sixty three (63) samples of Groundnut kernels from three (3) Agricultural zones of Sokoto State, were analysed for the presence of Aflatoxins B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) using High Performance Liquid Chromatography (HPLC). The Aflatoxin was detected in 82.5%, the total Aflatoxin concentration ranged between 0.9-646.0 μg/kg. All four types of AF were detected from all samples, out of the total samples analysed 38.10% contained AF levels above the Nigerian (20 μg/kg) limits for AF, while 66.7% samples had AF concentrations above the European Union maximum tolerance level of 2μg/kg for AFB1 and Total Aflatoxin(Tot AF) of 4μg/kg. The result shows that, there were significant (P > 0.05) differences between the samples analysed in the state, as regards to AFB1. The results also revealed that Groundnut kernel in the study area have high frequency of contamination (82.5%). However, awareness on danger of Aflatoxin contamination by all stakeholders is the key in effective management of the problem.Relevant quality control units must be reactivated to assess the quality of the Groundnut kernels from which other products are made.
Key words: Aflatoxin, Contamination, Store Groundnut kernel and Agricultural zones.
Adebajo LO, Idowu, AA.andAdesanya OO (1994). Mycoflora and mycotoxins production in Nigerian corn and corn based snacks. Mycopathologia126:183–192.
Akano DA and Atanda, OO. (1990) The present Level of Aflatoxin in Nigeria Groundnut Cake (‘Kulikuli’).Letters in App. Microbiol. 10: 187-18.
AOAC International (2008) Official methods of analysis of AOAC International. 18th edition. Gaithersburg, MD, USA, Association of Analytical Communities.
Bankole SA, Ogunsanwo BM and Eseigbe DA. (2005) Aflatoxins in Nigerian dry-roasted Groundnuts Food Chem 89:503–506.
Bankole SA and Mabekoje OO.(2004) Mycoflora and Occurrence of Aflatoxin B1 in Dried Yam Chips from Markets in Ogun and Oyo States, Nigeria.Mycopathologia.157(1):111-115.
Chang AS, Sreedharan A, Schneider KR. (2013) Peanut and peanut products: a food safety perspective. Food Contr 32:296–303.
Cora IB, Angre D, Ronald EM (2005). Separation of aflatoxins by HPLC application.Agilent Technology publication 5989-3634EN www.agilent.com/chem, 16th August, 2006.
Domsch KH, Gams W and Anderson TH (2007). Compendium of Soil Fungi, IHW-Verlag, Eching.
Ehrlich KC, Lee LS (1984). Mycotoxin grain dust: method of analysis of aflatoxin, ochratoxin A, Zearalenone, Vomitoxin and secalomic acid. J. Assoc. Official Anal. Chem. 67: 963.
Ezekiel CN, Sombie JI (2014). Survey of aflatoxins and fungi in some commercial breakfast cereals and pastas retailed in Ogun State, Nigeria.Nat Sci ;12(6):27-32
Ezekiel, C. N., Sulyok, M., Babalola, D. A., Warth, B., Ezekiel, B. C. and Krska, R. (2013) Incidence and consumer awareness of toxigenicAspergillus section Flavi and Aflatoxin B1 in Groundnut cake from Nigeria. Food Cont. 30:596-601
FAOSTAT.(2013) Faostat homepage.www.faostat.fao. org/site/339/default.aspx [accessed February 27, 2013].
Farombi EO (2003). African Indigenous plants with chemotherapeutic potentials and biotechnological approach to production of bioactive prophylactic agents. Afr. J. Biotech. 2(12): 662-671.
Food and Agriculture Organization.(2004). Worldwide regulations for mycotoxins in food and feed in (2003).FAO Foodand Nutrition Paper no. 81. Rome: Food and Agriculture Organization, Pp. 1– 180.
Glenn AE (2007)MycotoxigenicFusariumspecies in animal feed.Anim Feed Sci Technol. 137(3-4): 213-240.
Junaidu AU (2005) Epidemiological Studies on Brucellosis in Sokoto State.PhD Thesis, Dept. of Vet.Public Health and Animal Prod.UsmanuDanfodiyo University, Sokoto.Pp 167
Kayode OF, Sulyok M, Fapohunda SO, Ezekiel NC, Krska R and Oguntona CRB (2013).Mycotoxin and fungal metabolites in groundnut and maize-based snacks from Nigeria,Food Additives and Contaminants; 6:4, 294-300.
Krska R, Welzing E and Boudra H (2007).Analysis of fusarium toxins in feed, Ani. Feed. Sci. Tech. 137:241-264
Leslie JF, Summerell BA (2006) TheFusariumLaboratory Manual. Iowa State University Press, Iowa. 400pp
Liu Y, Wu F (2010) Global burden of aflatoxin-induced hepatocellular carcinoma: a risk assessment Environmental Health Perspectives 118:818-824.
McDonald D (1964). Progress reports on research into Aflatoxin production in Groundnuts in Northern Nigeria. Samaru Miscellaneous Papers 2: 13p.
Mutegi CK, Ngugi HK, Hendriks SL, Jones RB. (2009) Prevalence and factors associated with Aflatoxin contamination of Groundnuts from Western Kenya. Int J Food Microbiol 130:27–34.
National Population Commission (NPC, 2007).Population Census Data, Nigeria, Federal Republic of Nigeria Official Gazette, National and State Provisional Total Census, Printed and Published in Federal Government Printer, Lagos, No 21 vol.94,
Odoemelam SA. andOsu CI (2009). Aflatoxin B1 contamination of some edible grains marketed in Nigeria. E-J. Chem. 6(2): 308-314
Ogunsanwo BM, Faboya OOP, Idowu OR, Lawal OS, Bankole SA. (2004) Effect of roasting on the Aflatoxin contents of Nigerian Groundnut seeds. Afri J Biotechnol 3:451–455.
Rachaputi NR, Wright GC, Kroschi S (2002). Management practices to minimise pre-harvest Aflatoxin contamination in Australian Groundnuts. Austr. J. Exp. Agric. 42: 595-605.
Singh K, Frisvad JC, Thrane U, Mathu SB (1982). An illustrated manual on identification of some seed borne Aspergilli, Fusaria, Penicillia and their mycotoxins. Heller up, Denmark: Danish Government, Institute of seed pathology for developing countries. P. 133.
Soler CMT, HoogenboomOlatinwo R, Diarra B, Waliyar F, Traore S. (2010) Groundnut contamination by Aspergillusflavus and Aflatoxin B1 in granaries of villages and markets of Mali, West Africa. J Food Agric Environ 8:195–203.
Waliyar F, Reddy SV and Kumar PL (2005) Estimation of Aspergillusflavus infection and Aflatoxin Contamination in Seeds: Laboratory Manual. International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502 324, India. 26pp.
Woodroof JG. (1984) Peanuts: Production, Processing, Products, 3rd edn. Westport, CN, TheAvi Publishing Company.
World Health Organization (WHO, 2006) Mycotoxin in African foods: Implications to Food Safety and Health. AFRO Food Safety Newsletter, World Health Organization Food safety (FOS).
Wu F. (2007). Measuring the economic impacts of Fusarium toxins in animal feeds Anim Feed SciTechnol. 137(3-4): 363-374.
Yameogo RT, Kassamba B (1999). Aspergillusflavusand Aflatoxin on tropical seeds used for snacks Arachis hypogaea, Balanites aegyptiaca and Sclerocaryabirrea. Trop. Sci. 39: 46-49.