Attaullah et al
Greener Journal of Agricultural Sciences Vol. 2 (5), pp. 212-219, September 2012
ISSN: 2276-7770 © 2011 Greener Journals
Comparison of Different Techniques for the Isolation of Rhizoctonia solani from Infected Potato Tubers.
1Attaullah1, 2Shaukat Hussain1,3Farhad Ali1,3Amjad Khan and *2Gulzar Khan
1Khyber Pukhtunkhwa Agriculture University Peshawar
2North West Institute of Plateau Biology Chinese Academy of sciences, Xining 810001, China
3Gomal University KPK, Dera Ismail Khan
*Corresponding Author’s email: gulzar_khan92 @ yahoo. com
The effect of
surface disinfection, temperatures, nutrients and chemicals
on sclerotial germination of Rhizoctonia solani from
potato tubers were studied on potato dextrose agar (PDA).
Germination occurred from tuber pieces bearing sclerotia
pre-treated for one min with 0.1% of mercuric chloride.
Tuber pieces pretreated with 0.5% and 1%of mercuric chloride
for various lengths of times, including 15 sec, 30 sec and
one min showed no sclerotial germination on PDA. Similarly
sclerotial germination did not occur after pretreated with
sodium hypochlorite for same concentrations and times.
Sclerotial germination was optimal at a temperature of 250 C
on PDA. Germination of sclerotia was best on PDA followed by
cornmeal agar (CMA). Minimum mycelial growth occurred on
water agar (WA) while sclerotial germination was minimal on
malt extract agar (MEA) after one week of inoculation. Tuber
pieces bearing sclerotia were also treated with 0.1 % of
mercuric chloride, sodium hypochlorite, potassium hydroxide
and ethanol for one min before inoculation but no sclerotial
germination occurred on PDA except from pieces treated with
Key Words: Sclerotial Germination, Mycelial Growth, Inoculation, PDA.