Greener Journal of Agricultural Sciences Vol. 7 (7), pp. 160-166, September 2017.
Manuscript Number: 090117117
Haematological and Serum Biochemical Indices of Growing Cockerels Fed Varied Levels of Azolla-Turmeric Mixture
Alagbe J. O.
Dan-malafia Farms Limited, Ibadan
This study was conducted to examine the haematological and some serum biochemical indices of growing Isa cockerels fed diets supplemented with Azolla- turmeric mixture. One hundred and twenty (120) four weeks old Isa white cockerels were divided into four groups with three replicates, each of ten birds in a completely randomized design. Treatment 1 was fed basal diet without Azolla – turmeric mixture (ATMP), treatment 2, 3 and 4 were fed basal diets supplemented with ATMP at levels 5, 10 and 15% respectively. Proximate analysis shows that Azolla powder contained 22.51% crude protein, 3.53% ether extracts, 14.13% ash, 18.15% crude fibre and 37.88% nitrogen free extract while turmeric powder contained 9.14% crude protein, 2.40% ether extracts, 8.69% ash, 4.10% crude fibre and 69.10% nitrogen free extracts. The basal diet was formulated to meet the nutritional requirements of birds according to NRC (1994).
All the hematological parameters evaluated : Pack cell volume (PCV), Haemoglobin (Hb), White blood cell (WBC), Red blood cell (RBC), Mean corpuscular haemoglobin concentration (MCHC), Mean corpuscular volume (MCV), Mean corpuscular haemoglobin (MCH) were not significantly(P>0.05) affected by the inclusion of ATMP. However, there were no significant (P>0.05) differences among all treatment groups in the values of total protein, albumin, globulin, alkaline phosphatase, uric acid, creatinine, SGPT and SGOT throughout the experiment which lasted for 90days. It was concluded that Azolla –turmeric mixture could be added up to 15% in the diet growing cockerels without any adverse effects on their blood profile.
Keywords: growing cockerels, haematological parameters, biochemical indices, Azolla-turmeric mixture.
Agarwal M, Walia S, Dhingra S, Khambay BPS (2001) Insect growth inhibition, antifeedant and antifungal activity of compounds isolated /derived from Zingiber officinale Roscoe (ginger) rhizomes. Pest Management Science 57:289-300.
Adeyemo, G. O., Ologhobo, A.D and Adebiyi, O. A (2010) The effect of graded levels of dietary methionine on the heamatology and serum biochemistry of broilers. Int. Journal of Poult. Sci 9(2): 158-161.
Azzez, O. I., Oyagbemi, A. A and Oyewale, J. O (2009) Diurnal fluctuation in heamatological parameters of domestic fowl in the hot humid tropics. Int. Journal of Poult. Sci 8(3): 247-251.
Alagbe, J.O (2017). Growth performance and blood parameters of weaner pigs fed diets supplemented with turmeric powder (TUP). Scholarly Journal of Agricultural Science Vol 7(2), Pages 57-61. March, 2017. Available online at http:www.scholarly-journals.com/SJAS ISSN 2276-7118 © 2017 Scholarly-Journals
Ajao, B. H., Ola, S. I., Adameji, O. V., Kolawole, R. F (2013) The relationship of ambient temperature and relative humidity to the thermorespiratory function of greater grass cutter. Proc. of the 18th Annual Conf. Animal Sci. Assoc. of Nig 92.
Adenkola, A. Y and Durotoye, L.A (2004) Haematological study during pre-partum periods in brown savannah does in Zaria, Nig. Proc. of 38th Annual Conf. of the Agric Soc. Of Nig. 538-540.
Ameen, S. A., Adedeji, O. S., Akingbade, A. A., Olayeni, T. B., Ojedapo, L. O and Aderinola, A (2007) The effects of different regimes on haematology parameters and immune status of commercial broilers in derived savannah zone of Nigeria. Proc. of 32nd Annual Conf. Nig. Soc. Of Ani. Prod. 146-148.
Alagbe, J.O (2016). Effect of feeding varying levels of tiger nuts (Cyperus esculentus) seed meal on the performance and blood profile of Weaner grass cutters. Scholarly Journal of Agricultural Science Vol 7(1), Pages 15-19. Feb, 2017. Available online at http:www.scholarly-journals.com/SJAS
Alalade, O.A and Iyayi, E. A (2006). Chemical composition and the feeding value of Azolla meal for egg type chicks. Int’l Jou. Of Poult. Sci. 5(2): 137-141.
Abdi-Hachesoo, B., Talebi, A and S. Asri-Rezaei (2011) Comparative study on blood profiles of Indigenous and Ross-308 Broiler breeders. Global Vet., 7:238-241
Campbell, J. R and Lasley , J. F (1975) The science of animal that serve man. 2nd edn McGraw Hill Book Company New York, USA 200-202.
Church, J. P., Judd, J. T., Young, C.W., Kebay, J. L and Kim, W. W (1984). Relationship among diet constituents and specific serum clinical components of subjects eating self selected diets. Afr. Jour. Clin. Nutr. 40:1338-1344.
Chattopadhyay, I., Biswas, K., Bandyopadhyay, U and Banerjee, R.K (2004) Turmeric and Curcumin: Biological actions and medicinal applications. Curr Sci. 87:44-53.
Dein, F. J (1984) Laboratory manual of avian heamatology. Association of Avian Veterinarian , East North Port, USA.
Daramola, J. O., Adeloye, A. A., Fatoba, T. A and Soladoye, A. O (2005) Haematological and biochemical parameters of WAD goats. Livestock Res. For Rural Dev. 17(8) Available at: http://www.irrd.org17/8/clara/17095.htm.
Etim, N. N., Enyenihi, G. E., Akpabio, U and Offiong, E. E (2014a) Effects of nutrition on the heamatology of rabbits: A review. European Scientific Journal, 10(3):413-424
Ekenyem (2001) Response of broiler chicks to diets supplemented with garlic essential oil as natural growth promoter. Int. Journal of Science and Research Vol3 Issue 5 May 2001.
Swenson, M. J (1970) Physiologic properties, cellular and chemical constituents of blood. In: Dukes’ Physiology of Domestic Animals, 8th Edition Comstock publishing Associates, Cornell University Press, Ithaca and London, UK. Pp 21-26
Olorede, B. R and Longe, O. G (2002) Effect of replacing palm kernel cake with sheabutter cake on egg quality and serum chemistry of laying hens. Nig. Journal. Prod. 27: 19 – 23.
Olabanji, R. O., Farinu, G. O., Akinlade, J. A and Ojebiyi, O. O (2007) Growth performance and heamatological characteristics of weaner rabbits fed cyanide in processed cassava peel meals on heamatological and biochemical indices of growing rabbits. Proc. of the 35th Annual Conf. Nigeria Society of Anim. Prod. 212.
Iyayi, E. A (1994). Supplemental effect of low and high cyanide cassava on the performance, nutrient digestibility and serum metabolites of growing pigs. J. Agric. Trop. Sub-trop 95:199-205.
Ikepeama, N., Tayyem, R.F and Rock, C.L (2014). Curcumin content of turmeric and curry powders. Nutr.Cancer.55(2) 126-31.
NRC, National Research Council (1994) Nutrients Requirements of Poultry. (9th rev. Ed). National Academy Press. Washington D.C USA.
Huda-faujan., Noriham, A., Norrakiah, A and Babji, A (2009). Antioxidants activity in plants methanoic extracts containing phenolic compounds. African Journal of Biotechnology 8(3): 484-489.
Isaac, O.S (2013). Haematological parameters and factors affecting their values, Published by Science and
Education Centre of North America. Agricultural science Volume 2, Issue 1 (2014), 37-47. ISSN 2291-4471.
Kececi, T and R. Col (2011) Haematological and biochemical values of the blood of pheasants of different ages. Turkish Journal of Veterinary and Animal Science. 35:149-156.
Kamal, M.E Youssef., Amira M. El Newihi., Soad M. Omar and Zeinab S. Ahmed (2014) Assessment of proximate chemical composition, nutritional status and antioxidants of curcumin and mustard seed powder. Food and Public Health 2014, 4(6): 286-292.
Duncan, D.B. (1955). Multiple Range and Multiple F-Test Biometrics 11:1-42
Ibrahim Albokhadaim (2012). Haematological and some biochemical values of indigenous chickens in Al-Ahsa, Saudi Arabia during summer season. Asian Journal of Poultry Sciences. 6(4):138-145, 2012.
Daramola, J. O., Adeloye, A. A., Fatoba, T. A and Soladoye, A. O (2005) Heamatological and serum biochemical parameters of WAD goats. Livestock. Res. For Rural Dvp. 17(8)
Etim NseAbasi N., Glory E Enyenihi., Uduak, Akpabio and Edem, E. A Effiong (2007). Heamatological parameters indicators of the physiological state of farm animals. British Journal of Science 10(1): 33-45.
Bolu, S.A.O., Sola-Ojo, F.E ., Olorunsanya, O.A and Idris, K (2009) Effect of graded levels of dried pawpaw seed on the performance, heamatology , serum biochemistry and carcass evaluation of broiler chicken. Int. Journal of Poultry Sci. 8(9):905-909, 2009.
Talebi, A., Asri-Rezaei, S., Rozeh-Chai, R and Sahraei, R (2005). Comparative studies on haematological values of broiler strains ( Ross, Cobb, Arbo-acres and Arian). Int’l Journal of Poultry Sci., 4:573-579.
Togun, V. A., Oseni, B.S. A., Ogundipe, J.A., Arewa, T. R., Hammed, A. A., Ajonijebu, D.C., Oyeniran, A., Nwosisi, I and Mustapha, F (2007) Effects of Chronic Lead Administration on the Haematological Parameters of Rabbits- A Preliminary Study. Proc of the 41st Conf. of the Agric. Soc. Of Nig., 341
Ritchie, B.W., J. G. Harrison and R.L. Harrison (1994) Avian Medicine, Wingers Publishing Inc. Florida.
Reitman, S and Frankel, S (1957) Method for serum analysis using the colorimetric SGOT/SGPT assay. Animal Journal of Clinical Pathology.
SAS Institute (1994) SAS / STAT users guide. Version 6.1, 3rd Ed. SAS Inst., Cary, N.C
Scott, H.M (1965) Measurement of the amino acid content of fish meal protein by chick growth assay. Poult. Sci Pp: 365-395
Saleh, J. L., Njidda, A. A., Adeniji, A.A and Lawan, G. B (2014) Haematological and Biochemical Indices of Rabbits fed Graded Levels of Browse Forage in Semi Arid Environment. Global Journals Inc (2014).
Wikivet. Haematology ; 2013. Available at: www.enwikipedia/wiki/haematology
Tyras Rini Saraswati., Wasmen Manalu., Damiana Rita Ekastuti and Nastiti Kusumorini (2013) Increased egg production of Japanese quail by improving liver function through turmeric powder supplementation. International Journal of Poultry Science 12(10): 601-614 (2013).
Kurtoglu, F., Kurtoglu, V., Celik, I., Kececi, I and Nizamlioglu, M (2005) Effect of dietary boron supplementation on some biochemical parameters, peripheral blood lymphocytes, splenic plasma cells and bone characteristics of broiler chicks given diets with adequate or inadequate cholecalciferol content. Br. Poultry Sci., 46:87-96.
Onyeanusi, L. (2007). Hematological and Serological Characteristics of Cockerels Fed Different Levels of Tiger nut seed meal. International Journal of Multidisciplinary Research and Review .Vol. 1 (1): 23-27.
Mangala, H.S and Mathew, G.R (1986) Direct feed microbials and their direct impact on the intestinal micro flora and immune system of chickens. Journal of Poultry Science 47:106-114.
Yanagita, K.., Shiraishi, J., Kawakami, S and Bungo, T (2011) Time course changes in the blood parameters and expression of diencephalic CRH and AVT mRNA due to acute isolation stress in chicks. J. Poult. Sci 48(2): 125-129.
Call for Articles/Books
Call for Scholarly Articles
Authors from around the world are invited to send scholary articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles daily, immediately they are ready.
The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.
Use our quick submit button to submit or simply send your article(s) as an e-mail attachment to firstname.lastname@example.org or email@example.com.
Call for Books
You are also invited to submit your books for online or print publication. We publish books related to all academic subject areas. Submit as an e-mail attachment to firstname.lastname@example.org.