Shaba et al.
Greener Journal of Agricultural Sciences Vol. 1 (3), December 2011.
Trypanocidal potential of Camellia sinensis (Green Tea)
Shaba, P.1*, Pandey, N.N. 1, Sharma, O.P. 2, Rao, J.R. 3, Singh, R.K. 4
1Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
2Indian Veterinary Research Institute, Regional Station, Palampur, Himachal Pradesh, (176061) India.
3Division of Parasitology, Indian Veterinary Research Institute, Izatnagar - 243 122, India.
4Indian Veterinary Research Institute, Regional Station, Mukteswar, Uttranchal, (263138) India.
Present corresponding Author's address: College of Agriculture, P. M. B. 109, Mokwa Niger State, Nigeria/ Africa.
*Corresponding Author: Email: firstname.lastname@example.org
In search for new trypanocidal compounds from natural source, methanolic plant extract (MPE) of C. sinensis leaves at different concentrations (250-1000 µg/ml) was evaluated against Trypansoma evansi on Vero cell line grown in Dulbecco’s Modified Eagle Medium (DMEM) at appropriate conditions. In vivo infectivity assessment of incubated extracts and medium with trypanosomes was carried out in mice. In vitro cytotoxicity of test extract at concentrations (100-1.56 µg/ml) was carried out on Vero cells. For in vivo test, six mice in a group were inoculated with trypanosomes (1x104 /ml). Mice were treated with extract at concentrations (12.5-200 mg/kg body weight) of C. sinensis leaves intraperitoneally 48 h post on set of parasitaemia. MPE of C. sinensis leaves exhibited antitrypanosomal activity both in forms of immobilization and killing of trypanosomes. At 250 µg/ml of the test extract, trypanosomes could not be detected at 4 h of incubation, which was comparable to the standard drug, diminazine aceturate (50 µg/ml,) at 4 h. Trypanosomes counts decreased in concentration and time –dependent manner with significant difference (P<0.05). In infectivity assessment, group of mice inoculated with contents of wells with apparently killed trypanosomes survived while, the other group of mice with reduced trypanosomes died of parasitaemia. Both MPE of C. sinensis leaves and diminazine aceturate (Berenil) were cytotoxic to Vero cell line in all concentrations except at 1.56-12.5 and 6.25-1.56 µg/ml. Mice treated with extract had maximum 6 days of survival compared to 3 days of untreated control.
Keywords: Medicinal plant: Camellia sinensis leaves, Trypanosoma evansi, In vitro and In vivo Trypanocidal activity, ln vitro cytotoxicity test.
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