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Enyiukwu et al

Greener Journal of Biochemistry and Biotechnology Vol. 1 (2), pp. 052-065, July 2014.

  2384-6321© 2013 Greener Journals

Review Paper

Manuscript Number: 070414288


Biology and Management Strategies of Cowpea Anthracnose Disease Caused by Colletrotrichum Species


 D.N. Enyiukwu*1, A.N. Awurum1, C.C. Ononuju1, J.A. Nwaneri2


1Department of Plant Health Management, Michael Okpara University of Agriculture, Umudike, PMB 7269 Umuahia Abia State.

2Minor Root Crop Programme, National Root Crop Research Institute (NRCRI) Umudike, P.M.B 7006 Umuahia, Abia State, Nigeria.


*Corresponding Author’s Email: enyidave2003 @gmail .com


Anthracnose remains an important biotic factor constraining the efficient production of cowpeas in tropical and sub-tropical regions of the world especially in Nigeria. Correct and accurate identification of its causal organism is essential for tailoring appropriate control or management techniques for this impediment. So far, many Colletotrichum organisms have been reported as responsible for causing this disease in the crop. It appears that majority of these reports were predicated on morphological and colony characteristics of the pathogens from culture studies and/or in some cases, on uncritical assumption of host-specificity of the pathogens. This makes for the identity of the causal organism of anthracnose in the crop to be generally unclear, confusing and a subject of much scientific debate. In the Colletotrichum patho-system, it is known that different species could cause anthracnose of the same host. Since isolates, show overlapping ranges of morphological, colony and phenotypic characteristics, molecular diagnostic approaches such as sequence analysis of the internal transcribed spacer (ITS) region (1.8S and 5.8S; 5.8S and 28S genes) and sequence analysis of β tubulin genes which offer comparative variability for resolving phylogenetic relationships of Colletotrichum species; as well as sequence analysis of introns from two genes (glutamine synthase and glyceraldehydes-3-phosphate) and MAT1-2 mating sequences which have allowed differentiation of isolates from species complexes could play vital roles in discriminating the causal organism(s). Though cultural strategies, tolerlant and resisitant varieties against the disease and chemical interventions are used in managing the disease; resistance have been reported to be only temporary due to variability of Colletotrichum pathogens. Chemical interventions are disadvantaged in being human and eco-health disruptive; in addition to the fact that Colletotrichum spp. have shown resistance to carbendazim, thiophenate-methyl and benomyl. This review peers into the economic importance of anthracnose of cowpeas, its causal agent(s), management strategies for the disease and elaborated the response of the anthracnose organisms to phyto-chemicals from tropical plants for Integrated Disease Management (IDM) programs.

Keywords: Anthracnose, Colletotrichum spp. Cowpea, Molecular identification, Characterization, IDM.


Abate T, Ajeigbe H, Boukar O, Fatokun C, Kamara A, Ndjeunga J, Ntare B (2011). Grain Legumes of Nigeria. Bulletin of Tropical Legumes—A Monthly Publication of the Tropical Legumes II Project Vol. 12, Dec. 2011

Adesegun EA,  Adebayo OS,  Fajinma O (2011). Evaluation of spices extracts for antifungal properties. Arch. Appl. Sci. Res. 3(5): 486-490.

Adegbite AA, Amusa AN (2008). The major economic field diseases of cowpea in the humid agro-ecologies of South-western Nigeria. Afr. J. Biotech. 7(25): 4705-4712.

Afanador-Kafuri L , Minz D,  Maymon D, Freeman S (2008). Characteriztin of isolates from tamarillo, pissiflora and mango in Columbia and identification of unique species from the genus. Phytopathology 93(5): 579-589.

Ajibade SR, Amusa AN (2001). Effects of some fungal diseases on some cowpea lines in the humid environment of South Western Nigeria. J. Sust, Agric. Environ. 2(3): 246-256.

Akem N (1994). Management of soybean diseases Series 17, IITA, Ibadan-Nigeria, 36pp.

Akinbode, AO,  Ikotun T (2008).  Evaluation of some bioagents and botanicals in in vitro control of Colletotrichum destructivum. Afr J. Biotech 7(7): 868-872.

Allen DJ, Thottappilly G, Emechebe AM, Singh BB (1998). Diseases of cowpea. In: the pathology of food and pasture legumes. Allen DJ  Lenne JM (eds). CABI/ICRISAT, 1998, pp 750.

Amadioha AC (2003). Evaluation of some plant leaf extracts against Colletotrichum lindemuthianum in cowpea. Acta Phytopathologica et Entomologica Hungarica 38: 259 – 265.

Amadioha AC, Obi VI (1998). Fungitoxic activity of extracts from Azadirachta indica on Colletotrichum lindemuthianum in cowpea.  J. Herbs, Spices, Medicinal Plants 6(): 33-40.

Amadioha AC, Obi VI (1999). Control of anthranose disease of cowpea by Cymbopogon citratus and Ocimum gratissimum. Acta Phytopathologica et Entomologica Hungarica 34 (1-2): 85-89. 

Amusa NA, Ikotun T, Osikanlu YOK (1994). Screening cowpea and soybean for resistance to anthracnoce and brown blotch diseases using phytotoxic metabolites. Afr. Crop Sci J. 2(2): 221-224.

Awurum AN (2014). Varietal screenimg and response of cowpea to some foliar diseases in Southeast Nigeria. J. Sust. Agric. Environ. In Press

Awurum AN, Ucheagwu PO (2013). Effects of duration of storage of Piper guineense, Monodora myristica and Xylopia aethiopica on the germination and incidence of seed-borne fungi of stored cowpea (Vigna unguiculata L.Walp) seed. Continental J. Biol. Sci. 6(1): 37-42.

Awurum AN, Enyiukwu DN (2013). Evaluation of the seed-dressing potentials of phytochemicals from \Carica papaya and Piper guineense on the germination of cowpea (Vigna unguiculata L. Walp) seeds and incidence of the seed-borne fungi. Continental Journal of Agricultural Science 7(1): 29-35.

Awurum AN, Okwu DE, Onyekoro PO (2005). In vitro and in vivo control of Colletotrichum destructivum in cowpea using some plant extracts. J. Sci., Tech and Envt. 5(1-2): 52 – 57

Aveling T (2007). Cowpea Pathology Research. Pretoria South, Africa. Pp 1-7.

Ayodele M, Kumar R (2011). Bean anthracnose. In: Fungi and cowpea. Retrieved June 10, 2011. 

Cano J, Guarro J, Gene J (2004). Molecular and morphological identification of Colletotrichum species of clinical interest. J. Clin. Microbiol. 42: 2450-2454.

Cannon PF, Bridge PD, Monte E (2000). Linking the past, present and future of Colletotrichum systematic. In: Colletotrichum host specifity, pathology and host-pathogen interaction. Prusky D  Freeman S  Dickman  MD (Eds). APS Press, Minesota USA pp 1-20.

Cannon PF, Damm U, Johnson PR, Weir BS (2012). Colleotrichum - current status and future direction. Sudies in Mycology CBS-KNAW Fungal Biodiversity Centre pp 22-33.

COPR (Centre for Overseas Pest Research) (1981). Pest Control in Tropical Grain Legumes. London, UK, COPR 63-105.

Cropgenebank Knowledge Base (2014). Fungi – Cowpea.

Davies DW, Oelke EA, Oplinger ES, Doll JD, Hanson CV, Putnam DH (2012). Cowpea Alternative field crops manual, pp 1-9. Retrieved July 13, 2013

Du M, Schardi CL, Nuckies EM, Vailancourt LJ (2005). Using mating-type sequences for improved phylogenetic resolution of species complexes. Mycologia 97(3): 641-658.

Edema R, Adipala E (1994). Cowpea diseases (1 and 2). Control of false smut and brown rust in Uganda using benomyl and mancozeb. East Afr. Agric. J. 60(1): 11-17.

Emechebe AM (1981). Brown blotch of cowpea in Northern Nigeria. Samaru J. Agric. Research. 1(1): 20-25.

Emechebe AM, Florini DA (1997). Fungal and bacterial diseases of cowpea. In: Singh, R. S., Morgan-Raj,Daswell, K. E. and Jackai, L.E.N (Eds). Advances in Cowpea Research. IITA Pp 176-183.

Emechebe AM, Lagoke STO (2002). Recent advances in research on cowpea diseases. In: Challenges and Opportunities for enhancing sustainable cowpea production. Fatokun, C. A., Tarawali, S. A., Singh, B. B., Kormawa, P. M. and Tamo, M. IITA, Ibadan-Nigeria, pp 94-123.

Enyiukwu DN (2011). Evaluation of the fungitoxic activity of some phytochemicals on the causal agent of anthracnose of cowpea Colletotrichum destructivum O’Gara. MSc Thesis, Department of Plant Health Management, MOUA Umudike, June 2011.

Enyiukwu DN, Awurum AN (2013a). Fungitoxic principles and antifungal activity of extracts from Carica papaya and Piper guineense on Colletotrichum destructivum. Continental J. Biol. Sci. 6(1): 29-36.

Enyiukwu DN, Awurum AN (2013b). Fungitoxic effects of Carica papaya and Piper guineense extracts against Colletotrichum destructivum in the glashouse. Continental J. Agric. Sci. 7(1): 23-28

Enyiukwu DN, Awurum AN, Nwaneri JA (2014). Efficacy of plant-derived pesticides in the control of myco-induced postharvest and storage rots of tubers and agricultural products: A review. Net J. Agric. Sci. 2(2): 30-46.

Enyiukwu DN, Awurum AN, Ononuju CC, Nwaneri JA (2014b). Significance of characterization of secondary metabolites from higher plants in phyto-disease management: A review. Int. J. Adv. Agric. Res. 2:  8-28.

Eno IP (2011). Field trial on the potential of Anacardium occidentalis for the control of cowpea anthracnose disease in Nigeria. MSc Thesis presented to the Department PHM, MOUAU, 2011.

Freeman S, Katan T, Shabi E (1998). Characterization of Colletotrichum species responsible for anthracnose disease of various fruits. Plant Disease (D-1998-0320-01F) 82(6): 596-605.

Freeman S, Minz D, Jurkevitch E, Maymon M, Shabi E (2000). Molecular analyses of Colletotrichum species from Almond and other fruits. Phytopathology (P-2000-0418-01R) 608-614.

Gourounti K, Lykeridou K, Protopapa E, Lazaris A (2008). Mechanisms of actions of organochlorine substances. Health science Journal 2(2): 89-98.   

Grahovac M, Indic D, Vukovic S, Hrustc J, Gvozdenac S, Mihajlovic M, Tanovic B, (2012). Morphological and ecological features as differentiation criteria for Colletotrichum species. Zemdirbyste Agriculture 99(2): 189-196.

Guerber JC, Liu B, Cornell JC, Johnson PR, (2003). Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns mtDNA and intron RFLPs and mating compatibility. Mycologia 95(5): 872-895. .

Hyde KD, Cai L, McKenzie EHC, Yang YL, Zhang JZ, Prishastuti H, (2009). Colletotrichum: a catalogue of confusion. Fungal Diversity Online 1-17

Infonet-Biovision (2014). Cowpea. www.infonet-biovision,org/default/.../crop...

Jagtap GP, Gavate DS, Dey U (2011). Control of Colletotrichum truncatum causing anthracnose and pod blight of Soybesn by aqueous leaf extracts, biocontrol agent and fungicides. 1(2):

Latunde-Dada AO, O’Cornell RJ, Nash C, Pring RJ, Lucas JA, Bailey JA (1996). Infectin process and the identity of the hemibiotrophic anthracnose fungus (Colletotrichum destructivum O’Gara) from cowpea (Vigna unguiculata L Walp.) Mycological Research 100: 1133-1141.

Latunde-Dada AO, Bailey JA, Lucas JA, (1997). Infection process of Colletotrichum destructivum O’Gara from lucerne (Medicago sativa L.). European J. Plant Pathol. 103(1); 35-41.

Liu D, (2012). Molecular detection of human fungal pathogens. Liu D (ed) CRC Press, P 408.

Lubbe CM, Dennman S, Cannon PF, Groenewald JZE,  Lamprecht SC,  Crous PW, (2004). Characterization of Colletotrichum species associated with diseases of Proteaceae. Mycologia 96(6): 1268-1279.

Manire CA  Rhinehart HL  Sutton DA  Thompson EH  Rinaldi MG  Buck JD  Jacobson E (2002). Disseminated mycotic infection caused by Colletotrichum acutatum in a Kemp’s Ridley sea turtle. J. Clin. Microbiol. 40(11): 4273-4280.

Masangwa JIG, Aveling TAS, Kritziger Q (2013). Screening of plant extracts for antifungal activities against Colletotrichum species of common bean (Phaseolus vulgaris) and cowpea (Vigna unguiculata L.)

Melotto M, Baladin RS, Tally JD (2000). Host-pathogen interaction and variability of C. lindemuthianum. In; Colletotrichum host specifity, pathology and host-pathogen interaction. Prusky D  Freeman S  Dickman  MD (Eds). APS Press, Minesota USA pp 346-361.

Mendiratta DK, Thanke D, Shukla AK (2005). Keratitis due to Colletotrichum dematium – a case report. Indian J. Med. Microbiol. 23(1): 56-58.

Mohammed A (2013). An overview of distribution, biology and management of Common bean anthracnose. J. Plant Pathol Microb. 4(8): 193-

Mogle, UP (2013). Efficacy of leaf extracts against the postharvest fungal pathogens of cowpea. Bioscience Discovery 4(1): 39-42.

Mogle UP, Maske SR (2012). Effects of some plant extracts against seedborne infection of Colletotrichum destrucivum on Vigna unguiculata L. Bioscience Discovery 3(2): 266-269.

Moses RT (2006). Biological and chemical control of fungal seedling diseases of cowpea.

Mukherjee SK,  Islam MR, Shahid SB (2011). Efficacy of some plant extracts on the mycelia growth of Colletotrichum gloeosporioides. J. Bangladesh Agric. Univ. 9(1): 43-47.

Obi, VI, Bariuso-Vargas JJ (2013). Effects of some botanical on Colletotrichum destructivum O’Gara of cowpea. Afr. J. Microbiol. 7(37): 4576-4581.

O’connell R, Herbert C, Screenivasaprasad S, Khatib M, Esquerre-Tugaye MT, Dumas B, (2004). A novel Arabidosis-Colletotrichum pathosystem for the molecular dissection of plant-fungal interactions. Mol.Plant Microbe Intreact. 17(3): 272-282

Onyeani C, Osunlaja S (2012). Comparative effects of Nigerian indigenous plants in the control of anthracnose disease of mango. Int. J. Sci Tech. Res. 1(5): 80-87.

Ogu GE, Owoeye AE (2013). In vitro control of anthracnose disease of cowpea (Vigna unguiculata L. Walp) caused by Colletotrichum destructivum O’Gara with Cyathula prostrate L. and Diodia scandens SW leaf extracts. J. Int. EthnoPharm. 2(1): 29-36.

Ononuju CC, Kpadobi UC (2008). Effect of plant leaf ash on the control of Meloidogyne incognita in soybean (Glycine max (L.) Merrill.). Indian Journal of nematology 38(1): 9-14.

Onwusiri YC (2000). Evaluation of the leaf and ash extract of Chromolaena odoratum and Carica papaya as systemic nematicides of plantain. A Thesis submitted to the department of Plant Health Management, MOUAU, July, 2000.

Oparaeke AM (2007). Toxicity and spraying schedules of biopesticdes prepared from Piper guineense against two cowpea pests. Plant Proct. Sci. 43(3): 103-108

Owolade BF, Oshikanlu YOK (1999). Evaluation of some plant extracts for the control of brown blotch disease of cowpea in Southwest Nigeria. J. Sust. Agric. Environ. 1(2): 198 - 202.

Pakeia YP (2006). Interaction between Colletotrichum dematium and cowpea.

Perfect S, Hughes HB, O’Cornell RJ, Green JR (1999). Colletotrichum: A model genus for studies on pathology and fungal-plant interactions. Fungal Gen. Biol. 27(2): 186-198.

Podilla GP, Rogers LM, Kolattukudy PE (1993). Chemical signals from avcado surface wax trigger germination and appressorium formation in Colletotrichum gloeosporium. Plant Pathol. 103: 267-272.

Prassana, KPR (1985). Seed health testing of cowpea with special reference to Colletotrichum lindemuthianum. Seed Science and Technology 13: 821-827.

Qureshi, SH, Bashir, M, Alam SS (1985). Anthracnose of cowpea--a new disease record in Pakistan. Tropical Grain Legume Bulletin 30: 26.

SADAFF (South Africa Department of Agriclture, Forestry and Fisheries) (2009).  Production guild- lines for cowpeas.DAIS, Pretoria, South Africa.Pp 1-15.

Sato T, Moriwaki J, Uzuhashi S, Degawa Y, Ono T, Nishimura K (2012). Molecular phylogenetic analyses and morphological re-examination of strains belonging to three rare Colletotrichum species in Japan. Microbiol. Cult. Coll. 28(2): 121-134.

Shen S, Goodwin P, Hsiang T (2001). Hemibiotrophic infection and identity of the fungus Colletotrichum destructivum, causing anthracnose of tobacco. Mycol. Res. 105(11): 1340-1347.

Shivaprakash MR, Appannavavar SB, Dhaliwal M, Gupta A, Gupta S, Gupta A, Chakrabarti A (2011). Colletorichum truncatum: an ususual pathogen causing mycotic endophthalmitis. J. Clin. Microbiol 49(8): 2894-2898.

Shovan LR, Bhuniyan MKA, Begun JA, Pervez Z (2008). In vitro control of Colletotichum dematium causing anthracnose of soybean by fungicides, plant extracts and Trichoderma haziarum. Int. J. Sustain. Crop Prod. 3(3): 10-17.

Singh BB, Ehlers JD, Sharma JD and Freire-Filho, FR (2002). .Recent progress in cowpea breeding. In: Changesand Opportunities for Enhancing Sustainable Cowpea Production. Fatokun, C. A.Tarawali, S. A., Singh, B. B. kormawa, P. M and Tamo, M. IITA  Press, Ibadan Pp 21-31.                        

Suprapta, DN (2012). Potentials of microbial antagonist as biocontrol agents against plant fungal pathogens. J. ISSAAS 18(2): 1-8.

Sutton BC (1992). The genus Glomerella and its anamorph Colletotrichum. In: Colletotrichum: biology, pathology and control (Eds Bailey JA and Jerger MJ) CABI, Wallinford, 1-26.

Swetlana Z, Stojanovic S, Ivanivic Z, Trkulja N, Dolovac N, Aleksic G, Balaz J (2010). Mophological amd molecular identification of Colletotrichum acutatum from tomato fruit. Pestic. Phytomed Belgrade 25(3): 231-239.

Taiga A (2009). Efficacy of selected plant extracts in the control of fungal dry rot of white yam (Dioscorea rotundata) tubers in Kogi State. American-Eurasian J. Sust. Agric. 3: 310 – 313.

Taiga A (2011). Comparative studies on the efficacy of some selected fungicidal aqueous plant extracts on yam tuber dry rot disease. Annals Biol. Res. 2(2): 332-336.

Thankur RP, Girish AG, Rao VP (2011). Fungi diseases of pigeon pea. In: Handbook of pigeon pea ICRISAT, Patencheru India. .

Timko, MP, Ehlers JD and Robert DA (2007). Cowpea genome mapping and molecular breeding in plants Vol. 3, Pulses, Sugar and Tuber Crops. Kole, C. (Ed). Springer-Verlag  Berlin. Pp 49-67.

Than PP, Prihastuti H, Phoulivong S, Taylor PWJ, Hyde K (2008). Review: Chilli anthracnose disease caused by Colletotrichum species. J. Zhengjiang Univ. Sci. B 9(10): 764-778.

Than PP, Jeewon R, Hyde KD, Pongsupasamit S, Mongkolporn O, Taylor PWJ (2008). Characterization and pathogenecity of Colletotrichum species associated with anthracnose disease of chilli (Capsicum spp) in Thailand. Plant Pathology 57(53): 562-572.

Tshovhote NJ, Nesavuni  AE,  Raphulu T,  Gous RM (2003). The chemical composition, energy and amino acid digestibility of cowpeas used in poultry nutrition. SA J. Anim. Sci. 33(1): 65-69.  

Viljoen A (2004). Status and prevalence of mycotoxins. Department of Plant Pathology, Stellenbosch University. Retrieved November 10, 2013

Waller JM, Bridge PD (2000). Recent advances in understanding Colletotrichum diseases of some tropical perennial crops. In: Colletotrichum host specifity, pathology and host-pathogen interaction. Prusky D  Freeman S  Dickman  MD (Eds). APS Press, Minesota USA pp 337-345.

Xie L, Zhang JZ, Wan Y and Hu DW (2010). Identification of Colletotrichum spp isolated strawberry in Zhejiang Province and Shangai City, China. J. Zhejiang Uni. Sci. (Biomed and Biotech) 11(1): 61-70.

Yegneswaran PP, Pai P, Bairy I, Bhandary S (2010). Colletotrichum graminicola keratitis: First case in India. Indian J. Ophthalmol. 58(5): 415-415

Zakaria L, Shahak S, Zakaria M, Salleh B (2009). Characterization of Colletotrichum species associated with anthracnose of banana. Tropical life Sci. Res. 20(2): 119-125.