Greener Journal of Biochemisty and Biotechnology

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Rexford and Opara

Greener Journal of Biochemistry and Biotechnology Vol. 1 (2), pp. 035-051, July 2014.

  2384-6321© 2013  

Research Paper

Manuscript Number: 0513014230


Study of Microbes Immobilized Monolithic Electrodes in Microbial Fuel Cell


 Nwahia Chiedozie Rexford and Prof. Charles C. Opara


Department of Chemical Engineering, University of Port Harcourt, Nigeria.


*Corresponding Author’s Email: nwahiachiedozie @yahoo .com


Microbes Immobilized Microbial Fuel Cell Anode (MIMFCA) was produced using baker’s yeast, sodium alginate, activated carbon and cement, and tested in a Microbial Fuel Cell (MFC) setup to determine its workability and to determine the most suitable ratio of carbon and alginate in the anode to enhance the MFC cell performance. Five different electrodes were prepared with varying carbon –alginate contents of 15/2, 8/4, 6/6, 4/8 and 1/10 respectively. Six dual-chambers MFC set-ups of five liters each by volumes were used for the study. Five were operated with each of the immobilized yeast electrodes as anode while the sixth which served as control was operated with the cement-carbon monolithic electrode (where the microbes are introduced in the MFC system and made to freely attach themselves to the electrode as the cell was run). Glucose solution was used as the substrate and potassium ferricyanide as the catholyte. The voltage developed in each cell as well as the corresponding current were monitored for 10 days under ambient temperature and a neutral pH of (6.6-7.0) using a digital multi-meter connected to the circuit. The cell with electrode of 6/6 composition of carbon-sodium alginate was observed to have the highest electrical output of 1.68volts and 0.75 mA with power density and current density of 332.2785 mW/m2 and 197.7848 mA/m2 respectively while the control cell gave the lowest electrical output of 0.38volts and 0.10mA with power density of 26.3713 mW/m2 and current density of 10.0211 mA/m2.

Keywords: Microbes Immobilized MFC anode, bakers yeast Immobilization, sodium alginate, cement-carbon electrode.


Brodelius P, Deus B and Mosbach K (1979). Immobilized plant cells for the production and transformation of natural products. FEBS Letters. 103: 93 - 97.

Bruce EL (2008). Introduction, in Microbial Fuel Cells. John Wiley &Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470258590.ch1.

Logan BE and Regan JM (2006). Microbial fuel cells-challenges and applications. Environ. Sci. Technol. 40: 5172–5180.

Mathuriya AS and Sharma VN (2009). Bioelectricity Production from Paper Industry Waste Using a Microbial Fuel Cell by Clostridium Specie. J. Biochem. Tech. 1(2):49-52.

Monod J (1949). The growth of bacterial cultures. Ann. Rev. Microbiol.3: 371-394.

Potter MC (1911). Electrical effects accompanying the decomposition of organic compounds. Pr OC. R SOC. Lond. B Bio. Sci. 84: 44-48.

Rabaey K, Lissens G, Siciliano SD and Verstraete W (2003).  A microbial fuel cell capable of converting glucose to electricity at high rate and efficiency. Biotechnology Letters, 25: 1531-1535.

Rao JR, Richter GJ, Vonsturm F and Weidlich E (1976). Performance of glucose electrodes and characteristics of different biofuel cell constructions. Bioelectrochem. Bioenerg.  3(1): 139-150.

Wang X, Feng Y J and Lee H (2008). Electricity Production from   Beer Brewery Wastewater Using Single Chamber Microbial Fuel Cell. WA Publishing, Water Science and Technology- WST. 57:1-7.

Williams PD and Mavituna F (1992). Immobilized Plant Cells, in Plant Biotechnology. Comprehensive Biotechnology, Second Supplement, Fowler.

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