Umar et al
Greener Journal of Biochemistry and Biotechnology Vol. 3 (1), pp. 001-011, February 2016.
2384-6321© 2016 Greener Journals
Manuscript Number: 050415064
Decolourization of Textile effluent using Mutant strains of Aspergillus niger and Penicillium spp
1Umar, U.Z., *1Anil, K. Sharma and 2Tijjani M.B.
1Department of Biotechnology, Maharishi Markandeshwar University, Mullana-Ambala, Haryana (India)-133207.
2 Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.
The research was aimed at screening the fungal species from textile effluent that can degrade/decolourize the effluent and modify the fungal isolates for better decolourization process, to acceptable standards, prior to discharge of effluent into receiving water body. Textile effluents were collected from a Textile Company, located at Challawa Industrial Estate, Kano, Nigeria. Physico-chemical parameters such as Temp, pH, Total dissolved solids, Total suspended solid and Heavy metals analyses e.g. Cd, Pb, Mn, Cu and Fe were determined. Eight (8) different fungal species were isolated. Aspergillus niger and Penicillium spp were selected; Aspergillus niger (AN) and Penicillium spp (PN) were modified with ultraviolet rays at 254nm using 20 min. The mutant organisms of A. niger (ANµ2) and Penicillium spp (PNµ2) had higher decolourization potential of 82.81% and 59.64% respectively. Highest potential were observed in consortium of these isolates, when urea fertilizer was added to the effluent, in which maximum decolourization of 98.64% was observed in the consortium of the two fungal isolates with rapid production of biomass of 7.14 mg/ml. The overwhelming pollution of Challawa River with textile effluent can be controlled by employing the combination of two mutant strains of Aspergillus niger and Penicillium spp.
Key words: Decolourization, Effluent, Heavy metals, Aspergillus niger, Penicillium spp.
Ado SA (2004). Studies on genetic improvement of Aspergillus niger –CA3 for amylase production. PhD thesis, Department of Microbiology, Ahmadu Bello University Zaria, Nigeria, 84-87.
Aleem A, Isar J and Malik A (2003). Impact of long term application of industrial Water on emergency of resistance trails in Azotobacter chococcum isolated from rhizospheric soil. Biores. Technol, 86: 65-68.
Atiken MD and Irvine RL (1989). Stability Testing of ligninase and Mn-peroxidase from Phanerochaete chysosporium. Biotechnol. Bioeng, 34:1251-1260.
Atlas RM and Unterman R (1996). Bioremediation. In Manual of Industrial Microbiology and biotechnology. Ameican Society of Microbiology Press, Washington D.C. 238-315.
Burnet JH (1976). Fundamentals of Mycology. Second edition, William Clowes and Sons, Limited, United States, 221-281.
Chaudhry TM, Hill L and Khan AG (1996). Bioremediation of Iron and Zinc contaminated dumped filter cake by microbes, plants and associated mycorrhizae. In: Bioremediation of Heavy Metals. Wong M and Baker AJ, CRC Press, Boca Raton, Florida, 275-285.
Clesceri LS, Greenberg EA and Trussel R (1989). Standard methods for examination of water, wastewater, 17th edition, APHA, AWWA, Washington, U.S.A,65-74.
Contreras-Romos SM, Alvarez-Berna D. Trujillo Tapia N and Dendooven L (2003). Composition of tannery effluent with cow manure and wheat straw. Biores Technol, 94: 223-228.
Devise H L (2002). A Guide to Identification of Fungi. 4thedition. ASM Press, Washington D. C. U.S.A, 5-33.
Diaz E (2008). Microbial Biodegration: Genomics and molecular Biology, 1st edition,Caister Academic Press, New York, 350.
Dong J L, Zhang YW and Zhang RH (2005). Influence of culture conditionson laccase production and isozyme pattern in the white-rot fungus. J Bas. Microbiol, 45: 190 – 198.
Ezeronye OU and Okerentugba PO (1999). Performance and efficiency of a yeast biofilter for the treatment of Nigerian fertilizer plant effluent. J Microbiol and Biotechnol, 15: 515- 516.
Fahr K, Wetzstein G, Grey R and Schosser D (1999). Degradation of 2, 4-dichlorophenol and pentachlorophenol by two brown-rot fungi. FEMS Microbiol, 175: 127-132.
Gabriel BG (2005). Taxonomical Atlas for Identification of Fungi. 3rd edition, John Wiley publication, Florida, U.S.A, 11-43.
Gillman JK (1957). A Manual of Soil Fungi. Iowa State University Press, Ames, Iowa. U.S.A, 16-28.
Glazer AN (1997). Microbial biotechnology concept and technologies for bioremediation in confined disposal facilities. Dredging operation and environmental research (DOER): USA Army Corps of Engineers, U.S.A, 46-68.
Glenn JK. and Gold MH (1989). Decolourization of several polymeric dyes by lignin-degrading basidiomycetes Phanerochaete chysosporium. Appl. Environ, Microbiol, 45: 1741-1747.
Hardman DJ, McEldowney S and Waite S (1993). Pollution Ecology and Biotreatment. Long Scientific and Technical Publishers, Singapore, 1050-1059.
Harley JP and Prescott LM (1993). Basic Laboratory and Culture Techniques, 2nd editin, WMC Brown Publisher. New York, U.S.A, 14-46.
Howell G (1990). Acid rain and acid waters. Ellis Harwood series in Environmental Science. Ellis Harwood Ltd. New York, 134-136.
Idise OE and Ameh J B (2010). Studies on petroleum- degrading potentials of wild and mutant strains of Bacillus spp, Isolated from an oil-contaminated soil in Abraka, Delta state, Nigeria. Bayero J Pure and Appl. Sci, 3: 176 – 179.
John W (1986). Introduction to Fungi. Secound edition, Cambridge University Press, London, 269-282.
Klinch MA (2002). Identification of Common Aspergillus species. Utrecht, Netherlands: Central Bereauvoor. Netherland, pp. 48-54.
Lowerence, HJ (2002). Basic knowledge and perspective of bioelimination of xenobiotics Compounds. J Biotechnol, 51: 285-295.
Manu B and Chandhari S (2002). Anaerobic decolourization of simulated textile waste water containing ago dyes. Biores. Technol, 82: 225 – 231.
Nelson MJ, Kinsella JV and Montoya T (1990). In situ biodegradation of TCE contaminated ground water. Environmental Programme. 9: 190-196.
Okerentugba PO and Ezeronye OU (2003). Petroleum degrading potentials of single and mixed microbial culture isolated from rivers and effluents in Nigeria. Afri. J Biotechnol, 9: 288-292.
Palleroni N J (1984). Bergey’s manual of systematic bacteriology. Kreig NR and Holt J G 2nd eds, Willam and Wlkins press, Baltimore, U.S .A, 24-36.
Pepper IL and Gerba C P (2004). Environmental microbiology. A laboratory manual, 2nd Plant nutrients. May 24-26th, 1992. Islamabad, Pakistan, 607-610.
Raper KB and Fannel DI (1965). The genus Aspergillus. William and Wilklin. Baltore, U.S.A, 32-41.
Rojek K, Roddick FA and Parkinson A (2004) Decolorization of natural and lignin-degrading basidiomycetes Phanerochaete chysosporium. Appl. Environ, 6: 32-45.
Rowlands RT (1984). Industrial strain improvement: Mutagenesis random screening procedures. Enzy and Microb. Technol, 6: 3-10.
Sabour AM, Rabie F H, Mostafa T and Hassan SA (2001) Impact of industrial waste water bodies in Mostorod area north greater Cairo. J Environ. Sci, 13: 485 – 490.
Sani RK, Azmi W and Banerjee UC (1998). Comparison of static and shake culture in decolourization of textile dyes and dye effluent by Phanerochaete chysosporium. Environ. Microbiol, 43: 85-89.
Sani RK, Peyton BM and Brown LT (2001). Copper-induced inhibition of growth Desulfovibrio desifuricans G20: Assessment of its toxicity and correlation with those of Zinc and Lead. Appl. Environ. Microbiol, 67: 4768-47.
Wagner S (1993). Improvement in product and processing to diminish environmental impact. COTTECH Conference, Raleigh, NC. November 11-12.
Wataru S, Miyashita T, Yokoyamata J and Arai M (1999). Isolation of azo dye degrading microorganisms and their application to white discharge printing fibric. J Biol. Sci. and Bioeng, 88: 577 – 581.