Greener Journal of Biological Sciences Vol. 3 (1), pp. 048-057, January 2013
© 2011 Greener Journals
Manuscript Number: 021013442
Increase on ethanol production by blocking the ADH2 gene expression in GFP3-transformed Saccharomyces cerevisiae
Gomes L.H. 1, Duarte K.M.R.2*, Lira S.P.1
1,3Universidade de São Paulo – ESALQ – Depto. Exact Sciences – Chemistry- Lab of Natural Products . Av. Pádua Dias 11, 13418-900 Piracicaba, SP, Brazil
2*Institute of Animal Science and Pastures – CPDGRA R. Heitor Penteado, 56, 13460-000, Nova Odessa, SP, Brazil.
*Corresponding Author´s Email: keila @iz.sp. gov.br
Saccharomyces cerevisiae was transformed with plasmid pYGFP3, spelling the GFP3 gene fromAequorea victoria whose expression is modulated by the enzyme promoter ADH2 yeast gene.The plasmid pYGFP3 (GFP3, TRP1+, AMP) was re-constructed properly and used to transform the strain X2904-3C (met-,ura- trp1-). The transformed yeast strain X2904-3C-GFP3 produced 10.49 % (w/v) ethanol and ADH activity of 5.94 EUA.ml-1, compared to the non-transformed yeast X2904-3C, with 9.49 % (w/v) of ethanol and ADH activity of 9.41 EUA.ml-1, after 16 hours of fermentation These differences can be explained by the ADH2 activity blocked in the transformed yeast, converting less ethanol to ATP at the end of fermentation process.
Keywords: Alcohol deshidrogenase- 2; fermentation; green fluorescent protein; Saccharomyces cerevisiae.