Greener Journal of Biological Sciences

Excellence and Timeliness

  
  

Choose Language

Social


Greener Journal of  Biological Sciences Vol. 3 (6), pp. 213-219, August 2013.

 © 2011 Greener Journals

Research Paper

Manuscript Number: 041613579

 

Qualitative Assessment of Livestock Faeces and Sewage for Antibiotic Resistant Strains of E. coli in Roma, Lesotho

 

Mekbib S.B.*, Marole T.A. and Asita A.O.

 

Department of Biology, National University of Lesotho.

 

*Corresponding Author’s Email: sbmekbib @ nul.ls/sbmekbib7 @ gmail. com,

Tel: (+266) 63242329; (+266) 52340000


Abstract:

Sewage water (SW) and fresh livestock faeces: Chicken (ChNF), Sheep (SF), and Cattle (CF) were taken randomly from the farm of the National University of Lesotho aseptically during the 2012 academic year. Twenty ml of sewage water or 20 grams of fresh animal faeces was dissolved in 80 ml of sterilized Ringer’s solution and serially diluted. A 0.1 ml of sample suspension at 10-4 dilution was spread plated on Nutrient Agar (NA) and Eosine Methyl Blue (EMB) and plates were incubated for 24-48h at 24 and 37°C, respectively. A disc diffusion method was used to test the reaction of selected strains of Enterobacteriaceaeto nine antibiotics. In total, 24 strains under Enterobacteriaceae were isolated from the respective sources and the number of each that were Escherichia coli, as confirmed by the biochemical tests were: SW (7 and 3); ChNF (5 and 3); SF (8 and 6) and CF (4 and 2). Thirteen E. coli strains fermented lactose and were Catalase positive, while only one E. colistrain from SW was non-lactose fermenter and Catalase negative. The lethal potencies, as number of isolates sensitive to the 9 antibiotics, ranked as: Colistin = Amikacin (24/24) > Kanamycin (23/24) > Chloramphenicol (22/24) > Cefotaxime (21/24) > Sulphafurazole (7/24) > Methicillin (1/24) > Penicillin G = Rifampicin (0/24). Sensitivity of the E. coli strains ranked as follows, Colistin = Amikacin = Kanamycin (14/14) > Chloramphenicol (13/14) > Cefotaxime (12/14) > Sulphafurazole (6/14) > Methicillin (1/14) > Penicillin G = Rifampicin (0/14). This result showed that the development of antibiotic resistant E. coli strains, which can pass through food chain to humans and other organisms if stringent control measures are not taken. Strict quarantine procedures have to be applied to control such potential health risks.

Keywords: Escherichia Coli; Antibiotics; Sewage water; Entrobacteriaceae; Resistance.

Return to Content         View Reprint (PDF) (924KB)


Call for Papers/Books/Thesis

Call for Scholarly Articles


Authors from around the world are invited to send scholary articles that suits the scope of this journal. The journal is currently open to submissions and will process and publish articles monthly in two yearly issues.


The journal is centered on quality and goes about its processes in a very timely fashion. Seasoned editors/reviewers will be consulted to review each article(s), profer quality evaluations and polish the articles with expertise before publication.


Simply send your article(s) as an e-mail attachment to manuscripts@acad.gjournals.org or manuscripts.igj@gmail.com.


Call for Books


You are also invited to submit your books for online or print publication. We publish books related to all academic subject areas.    Submit as an e-mail attachment to books@journals.acadpub.online.


Call for Thesis

             



Search

Login Form

Other Journals


Newsletter


Sponsored