Greener Journal of Biological Sciences Vol. 3 (9), pp. 325-329, November 2013.
© 2011 Greener Journals
Manuscript Number: 280913872
OprD Expression and Imipenem Resistance in Pseudomonas aeruginosa
Hadeel Kareem Musafer1 and
Harith Jabbar Fahad Al-Mathkhury*2
Majeed Arsheed Sabbah3
1Department of Biology, College of Science, University of Mustansiriyah, Baghdad, Iraq.
2Department of Biology, College of Science, University of Baghdad, Baghdad, 10071 Iraq.
3Biotechnology Research Centre, Al-Nahrain University, Baghdad, Iraq.
*Corresponding Author’s Email: harithfahad @ scbaghdad.edu.iq, Mobile: 00964 7712277025
Pseudomonas aeruginosa is an opportunistic pathogen that produces highly resistant to antibiotic treatment. Here we show a presence of correlation between oprD expression and imipenem resistance. Minimal inhibitory concentration (MIC) for planktonic cells of P. aeruginosa was measured using E test. The results revealed the presence of oprD expression in 10 strains of P. aeruginosa isolates from patients with cystic fibrosis in order to evaluate their impact on imipenem susceptibility profiles. We investigated the relation between oprDgene expression and imipenem susceptibility profile showed isolates. Surprisingly, the imipenem-susceptible (P8) has low oprD expression that appeared in imipenem-susceptibleindicated a correlation of other mechanisms. This work forms a basis for future studies revealing the mechanisms of imipenem resistance in P. aeruginosa.
Keywords: Imipenem, oprD, Pseudomonas aeruginosa.