Greener Journal of Biological Sciences Vol. 4 (1), pp. 001-008, February 2014.
© 2011 Greener Journals
Manuscript Number: 071113720
Investigation of the Genetic Diversity of Selected Wild and Cultivated SorghumGermplasm Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis
Atoyebi O. J.1*, Faluyi J. O.2, and Oyedapo O. O.2
1National Centre for Genetic Resources and Biotechnology, Moor-Plantation, Ibadan, Nigeria.
2Department of Botany, Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
*Corresponding Author’s Email: johnatoyebi @ ymail. com
Thirty-five (35), wild (5) and cultivated (30) Sorghum bicolor germplasms were collected from 5 different agro-ecological zones in Nigeria. Proteins were extracted from six weeks old fresh leaves (1.0 g) with 10ml of 0.85% (w/v) sodium chloride (NaCl) solution, filtered and followed by centrifugation at room temperature at 3,000 rpm for 10 min. The supernatants were employed for protein analyses on polyacrylamide gel electrophoresis in the presence of SDS. The protein concentrations of the cultivars from North East, North West, South and wild were not statistically different ( P < 0.05) but in the same range with those of cultivars from North Central agro-ecological zone. Analyses of proteins revealed a total of 141 protein bands produced by the cultivars, 21 % has relative molecular mass of 190kD, 24% (22kD), 21% (11kD) and 24% (10kD). However, proteins with 21kD and 26kD were specific to only cultivars from North Central and wild collections. The proportion of differentiated protein (Pp) ranged between 0.06 and 0.24 while the dendrogram constructs revealed little variation between the cultivated and wild sorghum accessions.
Keywords: Sorghum bicolor, diversity, dendrogram, SDS- PAGE.