Greener Journal of Biological Sciences

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Greener Journal of  Biological Sciences Vol. 4 (5), pp. 146-153, June 2014.

 © 2014 Authors

Research Paper

Manuscript Number: 0511014228



PEGylated Gold Nanoparticles as a Biocompatible Contrast Agent in X-ray Imaging of Breast Tumor in Mouse Model


Mojtaba Salouti*1, Zahra Heidari1, Faranak Saghatchi2


1Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

2Department of Radiology, Faculty of Paramedical and Health Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.

*Corresponding Author’s Email: saloutim @yahoo .com, Fax: (+98) 241 4224024


Objective: Though biopsy is ultimately the gold standard for breast cancer detection, medical imaging can be a valuable tool for gathering information in vivo for both screening and diagnosis. In this study, we propose the use of gold nanoparticles (GNPs) modified with polyethylene glycol (PEG) as a biocompatible contrast agent in X- ray imaging of breast cancer in mouse model.

Methods: Gold nanoparticles in spherical shape were coated with PEG and the interaction was investigated by UV/vis spectroscopy. The UV/vis analysis was performed to check the stability of PEG-GNPs complex in human blood serum. The biodistribution and X–ray imaging studies were performed to evaluate the localization of PEGylated GNPs in xenografted breast tumor in mouse model.


Results: The interaction of PEG with GNPs was confirmed by UV–vis spectroscopy. The stability of PEG-GNPs was found high at human blood serum with no aggregation. The biodistribution study showed the accumulation of PEGylated GNPs in the site of tumor. The tumor was properly visualized in X-ray images in mouse model.


Conclusions: The results showed the potential of PEGylated gold nanoparticles as a contrast agent for X-ray imaging of breast cancer in humans that needs further investigations.

Keywords: Breast Cancer; X-ray Imaging; Contrast Agent; Gold Nanoparticles; PEGylation.


Brigger I, Dubernet C and Couvreur P (2002). Nanoparticles in cancer therapy and diagnosis. Advanced Drug Delivery Reviews. 54: 631–651.

Chanda N and Kattumuri V (2010). Bombesin functionalized gold nanoparticles show in vitro and in vivo cancer receptor specificity. PNAS. 107: 8760–8765.

De Jong W and Hagens W (2008). Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials. 29: 1912-1919.

Ding A and Chen Y (2008). HER-2 Antibody Conjugated Gold NanoRod particles for in vivo Photothermal Therapy. IEEE. 882-885.

Eghtedari M and Liopo AV (2009). Engineering of Hetero-Functional Gold Nanorods for the in vivo Molecular Targeting of Breast Cancer Cells. NANO  LET. 9: 287-291.

Fura A, Harper TW, Zhang H, Fung L and Shyu WC (2003). Shift in pH of biological fluids during storage and processing: effect on bioanalysis. Journal of Pharmaceutical and Biomedical Analysis. 32(3):513-522.

Ganeshchandra S and Keishiro T (2008). Biodistribution of colloidal gold nanoparticles after intravenous administration: Effect of particle size. Colloids and Surfaces B: Biointerfaces. 66: 274–280.

Gokce G, Citil M, Gunes V, and Atalan G (2004). Effect of time delay and storage temperature on blood gas and acid-base values of bovine venous blood. Research in Veterinary Science. 76(2):121-127.

Gunasekera UA and Pankhurst QA (2009). Imaging applications of nanotechnology in cancer. Targ Oncol. 4:169–181.

Hainfeld J F and Slatkin D N (2006). Gold nanoparticles: a new X-ray contrast agent, The British Journal of Radiology. (79) 248–253.

Heidari Z and Salouti M (2012). Targeting Molecular Imaging of Breast Cancer by Radioimmunodetection Method in Nuclear Medicine. Current Molecular Imaging. 1: 26-43.

Huang X and El-Sayed I (2006). Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold Nanorods. J. AM. CHEM. SOC. 128: 2115-2120.

Huang X and El-Sayed M (2010). Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy. Journal of Advanced Research. 1: 13–28.

Hutter E and Maysinger D (2010).  Gold Nanoparticles and Quantum Dots for Bioimaging. Microscopy Research and Technique. 1-13.

James X., Jie Z. and Jing Y., 2007. Gold-based nanoparticles for breast cancer diagnosis and treatment. IEEE. 2882-2885.

Kairemo K and Erba P (2008). Nanoparticles in Cancer. Current Radiopharmaceuticals. 1: 30-36.

Kircher MF, Zerda A and Jokerst JV (2012). Brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle. NAT MED. 18: 829–834.

Mironava T, Hadjiargyrou M, Simon M and Rafailovich M (2013). Gold nanoparticles cellular toxicity and recovery: Adipose derived stromal cells. Nanotoxicology. 1: 769128.

Natalie P, Praetorius TK and Mandal (2007). Engineered Nanoparticles in Cancer Therapy. Recent Patents on Drug Delivery & Formulation. 1: 37-51.

Niidome T and Yamagata M (2006). PEG-modified gold nanorods with a stealth character for in vivo applications. Journal of Controlled Release.  114: 343–347.

Popovtzer R and Agrawal A (2008). Targeted Gold Nanoparticles Enable Molecular CT Imaging of Cancer. NANO LET. 8(12): 4593-459.

Rabin O and Perez JM (2007). An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles, nature materials, 6: 118-122.

Salouti M., Babaei M.H., 2010. Comparison of 99mTc-labeled PR81 and its F (ab0)2 fragments as radioimmunoscintigraphy agents for breast cancer imaging. Ann Nucl Med. 1-6

Sungsook A, Sung YJ and Sang JL (2013). Review: Gold Nanoparticle Contrast Agents in Advanced X-ray Imaging Technologies, Molecules.  18: 5858-5890.

Tong L and Wei Q (2009). Gold nanorods as contrast agents for biological imaging: optical properties, surface conjugation, and photothermal effects. Photochem Photobiol. 85(1):1- 21.

Wang J, Liuand D and Wang Z (2011).  Synthesis and cell-surface binding of lectin-gold nanoparticle conjugates, The Royal Society of Chemistry.

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