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Greener Journal of  Biological Sciences Vol. 5 (1), pp. 010-019, March 2015.

 © 2015 Greener Journals

Research Paper

Manuscript Number: 012115007


DOI: http://doi.org/10.15580/GJBS.2015.1.012115007)

 

Detection of aminoglycoside modifying enzymes and β-lactamases in Acinetobacter baumannii isolated from hospitalized Iraqi patients

 

Israa Mohamed Safi Al-kadmy1; Marwah Hasan Al-Kaabi2; Hadeel Kareem Musafer1

 

1Department of Biology, College of Science, University of Mustansiriya, Baghdad 14022, Iraq.

2National center of Hematology, University of Mustansiriya, Baghdad, Iraq.


Corresponding Author’s Email: israaalkadmy @gmail. com



Abstract


Background/aim: Acinetobacter baumannii is one of the most commonly encountered as multiresistance microorganism. This study was designated for investigating of β-lactamases resistance genes and aminoglycosides modifying enzymes (AMEs) in Acinetobacter baumannii isolated from Iraqi patients.

 

Materials and methods: A total of 36 A. baumannii isolates were recovered from hospitalized Iraqi patients during May to September 2012. Antibiotic susceptibility test was performed against aminoglycosides antibiotics and phenotypic detection of ß-lactamases was done using Extended Spectrum Beta Lacatmases (ESβL) strip. Polymerase Chain Reaction (PCR) amplification was achieved for investigating β-lactamases genes (blaSHV, blaTEM, and blaCTX) and aminoglycosides modifying enzymes (aac(6)-Ib, ant(4')-IIb, aa(c) 3′ and  aph(3′)-VI).

 

Results: Out of 36 A. baumannii isolates, 15 (41.66%) were resistant to aminoglycosides antibiotics according to disk diffusion method. 17 (47.22%)  isolates gave positive results in the preliminary screening of β-lactamases, the ESβLs type and AmpC enzyme, while 13 (36.11% ) of the isolates gave a  positive result in Metallo-β- lactamases production test. PCR analysis demonstrated that  blaTEM was detected in 3 (20%) of the resistant isolates , blaSHV was detected in 9 (60%) , blaCTX-M was detected in 11 (73.33%), aac (6)-Ib was detected in 7 (46.66%), ant(4')-IIb was detected in 5(33.33%) , aph(3′)-VI in 2 (13.33%), and aa(c) 3′ was found in 10 (66.66%) of the resistant isolates.

 

Conclusion: Local A. baumannii isolates with AMEs and β-lactamases genes represent a powerful nosocomial pathogen that threat the antibiotic era and life of immunocompromied and hospitalizes patients and this should be taken into account to find a new ways for the restriction of these powerful pathogens.

 

Key words: Aminoglycoside modifying enzymes, Acinetobacter baumannii, Iraq.



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