Greener Journal of Biological Sciences

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Greener Journal of  Biological Sciences Vol. 6 (5), pp. 089-094, November 2016.

 © 2016 Authors

Research Paper

Manuscript Number: 101916181



Impact of Water Soluble Fractions of Crude Oil on Growth Performance of Ptychadena mascariensis


Magdalene Okeh Nafagha-Lawal1, Francis David Sikoki2 and         Onwunari Abraham Georgewill3


1Research Associate, Center for Marine Pollution Monitoring and Seafood Safety, University of Port Harcourt, Rivers state.

2Professor, Department of Animal and Environmental Biology, University of Port Harcourt, Rivers state.

3 Professor, Department of Pharmacology, University of Port Harcourt, Rivers state.


Study on the impact of sub-lethal concentrations (0mg/L, 0.3gmg/L, 0.75mg/L, 1.5mg/L, 2.25mg/L and 3.0mg/L) of water-soluble fractions (WSFs) of crude oil on the growth performance of the frog Ptychadena mascariensis tadpoles was carried out under laboratory conditions in the University of Port Harcourt, during a 12-week period of exposure in a renewal static bioassay system. Growth of P. mascariensis was observed to reduce significantly with increasing time of exposure and concentrations of WSFs of crude oil when compared with the controls. Weight gain and SGR were all significantly reduced in the test group compared to the control group (p<0.05). However, the length gain was not significantly reduced (P>0.05).  The regression co-efficient obtained for the length-weight relationship for the frogs in the control was 3.005, while for frogs were exposed to crude oil of various concentrations ranged from 2.0952 2.4369.  This suggested an isometric growth form in all specimens in the control and a negative allometric growth pattern for frogs exposed to crude oil. This study indicated that crude oil had a negative impact on the growth rates of and growth pattern P. mascariensis.


Keywords: Amphibians, bio-indicator, growth rates, length-weight relationship.

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