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Greener Journal of  Biological Sciences Vol. 7 (3), pp. 025-033, May 2017.

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Research Articles

Manuscript Number: 032017040



Qualitative and Quantitative Changes of Haemocytes from Honeybee, Apis mellifera (L.) (Hymenoptera:

Apidae) After Intrahaemocoelic Injection with Bacillus thuringiensis


Emad M.S. Barakat*1, Walaa A. Moselhy2, Zeinab A. Shouaib3, Mostafa A. Taha4


1Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.

2, 3, 4Department of zoology, Faculty of Science, Al-Azhar University, Naser city, Cairo, Egypt.


The present work was carried out aiming to characterize the haemocytes from different developmental stages of honeybee, Apis mellifera (L.) after experimental injection of Bacillus thuringiensis (B.t.) into the haemocoel. Six haemocyte types were recognized in pupae: prohaemocytes (PRs), granulocytes (GRs), oenocytoides (OEs), spindle-shaped cells (SPLs), plasmatocytes (PLs), and adipohaemocytes (ADs). Eight types were found in larvae; in addition to the previous mentioned haemocyte types, spherulocytes (SPs) and eosinophil cells (EOs), while ten types were recognized in the adult stage; the previous mentioned eight types plus micronucleocytes (MIs) and macronucleocytes (MAs). Due to bacterial injection, certain pathological consequences were observed in the defined haemocytes such as vacuolization in the cytoplasm, distortion of the cell membrane and pycnosis in the nuclei. Moreover, the differential and total haemocyte counts were found to increased or decreased depending on the developmental stage and the time of haemolymph collection after bacterial injection. Other observed cellular responses against bacterial injection were phagocytosis and nodule formation.


Key words: Apis mellifera, Bacillus thuringiensis, haemolymph, haemocytes, THC, DHC, cellular immunity, phagocytosis, nodule formation.

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