Greener Journal of Biological Sciences

Open Access

Greener Journal of  Biological Sciences Vol. 7 (7), pp. 063-068, December 2017.

 © 2017 Greener Journals

Research Articles

Manuscript Number: 120517176



Biological Control of Water Pollution at Challawa Industrial Area Kumbotso Local Government, Kano using Moringa oleifera Seed Extract


Asiya A1 and *Ali M2


1Department of Basic Studies, College of Basic and Remedial Studies Hassan Usman Katsina Polytechnic Katsina State.

2Microbiology Department, Kano University of Science and Technology Wudil.


Water purification and reduction of water borne diseases is a major public health goal. The study was aimed to utilize Moringa oleifera seeds extract for purification of ground water at Challawa industrial area Kumbotso Local Government Kano State, Nigeria.  Ground water was collected from four different locations in the area. All water samples were subjected to bacteriological and physiological analysis using standard laboratory methods. Extract from the seeds of Moringa oleifera was used in the process of purifying the water as antibacterial and anticoagulant agent. The result showed that 5 bacterial isolates (Escherichia coli, Salmonella typhi, Proteus mirabilis, Enterobacter spp and Klebsiella pneumoneae) were recovered and all were susceptible to Moringa oleifera seeds extract. The most susceptible isolate is E. coli with aveage zone of inhibition of 17.82 mm while Klebsiella pneumoneae is the least susceptible with average zone of inhibition of 13.00 mm. The turbidity assessment showed that at a concentration of 1000mg/L the Moringa seed suspension can reduce the turbidity of ground water from 4NTU to 1NTU after exposing for 90 minutes. Moringa, therefore, could be regarded as one of the remedy to reduce the incidence of water borne disease leading to high incidence of death especially in the developing world.


Keywords: Moringa oleifera; bacteria, physicochemical; Extract

Post-review Rundown

View/get involved, click [Post-Review Page]


Ahmed, I. and Beg, A.Z. (2001). Antimicrobial and phytochemical studies on 45 Indian Medicinal plants against multi-drug resistance human pathogens. J Ethnopharmacol74, 113-123


Ali, M., Aminu, F., and Ibrahim, I. S. (2017). In-vitro Assessment of Antibacterial Activity and Phytochemical Screening of Vitex doniana on Clinical Isolate of Salmonella typhi International Journal of Advanced Academic Research | Science, Technology & Engineering V 3(1) PP 9-16


Castillo, G., Duarte, R., Ruiz, Z. Marucic, M. T., Honorato, B. Mercado, R., Coloma, V., Lorca, V. Martins, T. M. and Dutka, B. J. (1999).“Evaluation of Disinfected and Untreated Drinking Water Supplies in Chile by the H2S Paper Strip Test,” Water Research, Vol. 11, No. 28, pp. 1765- 1770. DsOI:10.1016/0043-1354(94)90249-6


Food and Agriculture Organisation (FAO) (1997).Chemical analysis manual for food and water, 5thEd,FAO rome, 1: 20-26


Genthe, B. and Franck, B. (1999). “A Tool for Assessing Microbial Quality in Small Community Water Supplies,” WRC Report Pages to Water Research Commission by Division of Water, Environment and Forestry Technology, CSIR, Stellenbosch, Vol. 33, No. 961/1/99


Ihekoronye, A.I. and Ngoddy, P.O. (1985).Integrated food science and technology for tropics.Oxford: Macmillan Press, pp. 95-195.


Jolt, J.G., Krieg, N.R., Sneath, P.H., Stanley, J.T. and Williams, S.T. (1994).Bergey’s manual of systematic bacteriology, 9th edn. Williams & Wilkins Co. Baltimore, Maryland, p786.


Kaspar, I. Guillen, D. Revelli, T. Meza, G. Velazquez, L. Mino de Kasper, C. Pozolli, C. Nunez and G. Zoulek, (1999). “Evaluation of a Simple Screening Test for the Quality of Drinking Water Systems,” Annals of Tropical Medicine and Parasitology, Vol. 3, No. 43, pp. 124-127.


Lamikanra, A. (1999). Essential microbiological for students and practitioners of pharmacy, medicine and microbiology, 2nd Edition, Amkra books, 406pp.


Mark D. S. and Frederic, K. P. (2003). “Evaluation of the H2S Method for Detection of Faecal Contamination of Drinking Water,” WHO/SDE/WSH/02.08 Geneva


Marque, S., Jacqmin-Gadda, H., Dartigues, J.F. and Commenges, D.(2003).Cardiovascular mortality and calcium and magnesium in drinking water: an ecological study in elderly people. European Journal of Epidemiology 18:305–309


Martins, M., Castillo and G. Dutka, B. J (1997). “Evaluation of Drinking Water Treatment Plant Efficiency in Microor- ganism Removal by the Coliphage, Total Coliform and H2S Paper Strip Test,” Water Science and Technology, Vol. 3, No. 35, pp. 403-407. doi:10.1016/S0273-1223(97)00293-X


Okonko,I.O., Adejoje, O.D., Ogunnusi, T.A., Fajobi, E. and Shittu, O.B. (2008). Microbiological and physicochemical analysis of different water samples used for domestic purposes in Abeokuta and Ojota, Lagos Nigeria. African Jounal of Biolechnology7(5) 617-6721.


Onuh, J.O. and Isaac, V.U. (2009).Physicochemical and microbiological quality of water sources from some major towns in Igala land. Nigeria food Journal27(22): 66.


Ranjini K; Roland P; John C. and Vincent R. (2010). Microbiological and physicochemical analysis of drinking water in George town. Nature and Science, 8 (8)261-265.


Rijal, G. K., Fujioka and R. S. and Ziel, C. A (2000). “Evaluation of the Hydrogen Sulfide Bacteria Test, a Simple Test to De- termine the Hygienic Quality of Drinking Water, Abstracts of the General meeting of the American Society for Microbiology,” Poster Presentation (Unpublished), American Society of Microbiology, Washington DC


Shittu, O. B, Olaitan, J.O. and Amusa, T.S. (2008).Physico-chemical and bacteriological analyses of water used for drinking and swimming purposes in Abeokuta, Nigeria. African Journal of Biomedical Research 11: 285-290.


Sobsey, M. D. (2001). “Microbial Detection: Implications for Exposure, Health Effects, and Control,” In: G. F. Craun, Ed, Safety of Water Disinfection: Balancing Chemical and Microbial Risks, Intl Life Sciences Inst Pres, Washington DC, pp. 11-51.


Tebutt T.H.Y. (1983). Principles of Quality Control. England: Pergamon, p.232. US Environmental protection Agency (USEPA) (1995).Guidelines for water suppl.y7th edition. New York: WHO press, pp.144 -162


Venkobachar, C., Kumar, D., Taireja, K., Kumar, A. and Iyengar, I. (1994). “Assessment of Bacteriological Quality Using a Modified H2S Strip test,” Aqua (Ottoro), Vol. 6, No. 43 pp. 311-314.


World Health Organization (2004). Guidelines for drinking water quality.3rd edition, Switzerland: WHO press pp.16,89