Greener Journal of Cell and Animal Biology

Open Access

Cantinha et al

Greener Journal of Cell and Animal Biology Vol. 1 (1), pp. 001-007, September 2013.

  ISSN: 2354 - 2322 © 2013 Greener Journals

Research Paper

Manuscript Number: EB08291301


Thermotolerance and induction of HSP70 in Biomphalaria glabrata (Say, 1818)


Rebeca Da Silva Cantinha1,2*, Sueli Ivone Borrely1,

Nancy Oguiura3, Adriano Polpo4,

Carlos Alberto De Bragança Pereira5, Eliana Nakano2


1Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Avenida Lineu Prestes, 2242,

Cidade Universitária, CEP 05508-000, São Paulo, SP, Brasil.

2Laboratório de Parasitologia, Instituto Butantan, Avenida Vital Brasil, 1500, Butantan, CEP 05503-900, São Paulo, SP, Brasil.

3Laboratório de Ecologia e Evolução, Instituto Butantan, Avenida Vital Brasil, 1500, Butantan, CEP 05503-900, São Paulo, SP, Brasil.

4Centro de Ciências Exatas e de Tecnologia, Departamento de Estatística, Universidade Federal de São Carlos, Via Washington Luís, km 235, CEP 13565-905, São Carlos, SP, Brasil.

5Departamento de Estatística, Instituto de Matemática e Estatística,Universidade de São Paulo, Rua do Matão, 1010, Cidade Universitária, CEP 05008-090, São Paulo, SP, Brasil.

 *Corresponding Author’s Email: rebecanuclear @


Thermotolerance is a temporary resistance against lethal temperatures. Induction of heat shock proteins (HSP), mainly HSP70, has been correlated to this response. Our research demonstrated the pre-exposure to sublethal temperatures of 30, 33 and 36 °C increased the survival of Biomphalaria glabrata to lethal temperature of 42 °C. This animal is a freshwater snail recognized as a suitable organism for experimental research. Additionally, it was observed an induction of HSP70 expression in digestive gland in all temperatures tested but not in ovotestis and head/foot tissues. Despite the higher expression of HSP70 had been detected at 30 °C, the animals exposed to 33 °C presented the highest survival. Our results indicate the increase of HSP70 is involved in the resistance to lethal temperature in B. glabrata as well other proteins. 

Keywords: Thermotolerance; Biomphalaria glabrata; HSP70.

HSP: Heat Shock Protein; HSP70: Heat Shock Protein of 70 kDa; B. glabrata: Biomphalaria glabrata; S. mansoni: Schistosoma mansoni.

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