Greener Journal of Epidemiology and Public Health Vol. 2 (1), pp. 016-022, July 2014.
ISSN: 2354-2381 © 2013 Greener Journals
Manuscript Number: 041814192
Comparative Molluscicidal Activities of Methanolic and Crude Extracts of Jatropha curcas Leaves against Biomphalaria pfeifferi
Tariwari C.N. Angaye*1, Douye V. Zige1, Baraikio Didi2,
Nengimonyo Biobelemoye2 and Endurance A. Gbodo3
1Toxicology Research Unit, Department of Biological Sciences, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
2Medical Microbiology/Parasitology Unit, Department of Medical Laboratory Science, University of Calabar, Cross River State, Nigeria.
3Department of Biological Sciences, Faculty of Science, Federal University Otuoke, Bayelsa State Nigeria.
*Corresponding Author’s Email: maktarry @yahoo .com
The bioassay of the molluscicidal Activities of J. curcas leaves against Biomphalaria pfeifferi was investigated in vitro, using Crude and 70% methanolic extracts in a two phased rapid and final screening test. The rapid screening of the crude extracts, for the minimal molluscicidal concentration (LC100), of the snails was less active (>500ppm), compared to the methanolic extracts (<500ppm). The final screening, revealed an LC50 and LC100 values of 6 and 30ppm respectively. Further investigation based on exposure, revealed a mortality within 12 hours, which meets WHO standard of less than 24 hours for a potential molluscicide. These results confirmed that J. curcas leaves can be regarded as a potential molluscicide for the control of schistosomiasis and therefore encourage the cultivation of this plant in endemic regions.
Keywords: Biomphalaria sp., Jathropha curcas, molluscicidal activities, schistosomiasis, rapid screening, final screening.
Agboola IO, Ajayi GO, Adesegun S.A., Adesanya S.A., (2011). Comparative Molluscicidal activity of fruit pericarp, leaves, seed and stem Bark of Blighia Unijugata Baker. Pharmacology Journal, 3, 63-66.
Ahirrao RA, Patel MR, Pokal DM, Patil JK, Suryawanshi HP, (2011). Phytochemical Screening of Jatropha curcas Plant. 2(4), 1314-1327.
Al-Zanbagi NA, Banaja AEA, Barrett J, (2000). Molluscicidal activity of some Saudi Arabian Euphorbiales against the snail Biomphalaria pfeifferi. Journal of Ethno-pharmacology, 70 119-125.
Ames BN, Profet M, Gold LS, (1990). Nature’s chemicals and synthetic chemicals: Comparative toxicology. Proceedinds of the National. Academy of Science USA, 87, 7782-7786.
Angaye, TC, (2013). In-vitro Comparative Molluscicidal Activities of Aqueous and Methanolic Extracts of Jatropha curcas Leaves Against Bulinus globosus and Bulinus rholfsi; vectors of urinary schistosomiasis. M.Sc. thesis, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
Bassey SE, Ohimain EI, Angaye TC, (2013). The Molluscicidal Activities of Methanolic and Aqueous Extracts of Jatropha curcas Leaves against Bulinus globosus and Bulinus rholfsi, Vectors of Urinary Schistosomiasis. Journal of Parasitology. Photon 103, 115-122.
Bassey SE, (2011). A Concise Dictionary of Parasitology. Zetu Concept, Port-Harcourt, Nigeria. 5, 89-96.
Bershadsky AD, Ivanova OY, Lyass LA, Pletyushkina OY, Vasilieiv JM, Gifand IM, (1990). Cytoskeletal reorganizations responsible for the phorbol ester-induced formation of cytoplasmic processes: possible involvement of intermediate filaments. Proceedings of the National Acadamy of Sciences USA 87, 1884-1888.
Buckingham J, (1994). Dictionary of natural products. London: Chapmann and Hall.
Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, Nishizuka Y, (1982). Direct activation of calcium-activated, phospholipiddependent protein kinase by tumor-promoting phorbol esters. Journal of Biochemistry, 257, 7847-7851.
Cheeke P, (1998). Natural toxicants in feeds, forages and poisonous plants. Danville, IL: Interstate. curcin, a ribosome-inactivating protein rom the seeds of Jatropha curcas. Acta Botanica Sinica, 45, 858-863.
Clerk TE, Appleton CC, Drewers SE, (1997). A semi-quantitative approach to the selection of appropriate candidates plant molluscicides-A South African application. Journal of Ethnopharmacology, 56, 1-13.
Devappa RK, Makkar, Harinder PS, Klaus B, (2010). Jatropha Toxicity-A Review. Journal of Toxicology and Environmental Health, Part B. 13(6), 476-507.
El Kheir YM, El Tohami MS, (1979). Investigation of molluscicidal activity of certain Sudanese plants used in folk-medicine. American Journal of Tropical Medicine and Hygiene 82, 237-241.
Guo D, Chen J, Du X, Han B, (2010). Screening of molluscicidal strain against Oncomelania hupensis from the rhizosphere of medicinal plant Phytolacca acinosa Roxb. Pharmacognosy Mag, 6(23), 159-165.
Heller J, (1996). Promoting the conservation and use of underutilized and neglected crops (Jatropha curcas L.). Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Hirota M, Suttajit M, Suguri H, (1988). A new tumor promoter from the seed oil of Jatropha curcas L., an intramolecular diester of 12-deoxy-16- hydroxyphorbol. Cancer Research 48, 5800-5804.
Igbinosa OO, Igbinosa EO, Aiyegoro OA, (2009). Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). African Journal of Pharmacy & Pharmacology 3(2), 58-62.
Makkar HPS, Becker K, (2009). Jatropha curcas, a promising crop for the generation of biodiesel and value-added coproducts. European Journal of Lipid Science Technology 111,773-787.
Makkar HPS, Becker K, Sporer F, Wink M, (1997). Studies on nutritive potential and toxic constituents of different provenances of Jatropha curcas. Journal of Agriculture & Food Chemistry 45, 3152–3157.
Nath LK, Dutta SK, (1997). Acute toxicity studies and wound healing response of curcain, a proteolytic enzyme extract from the latex of Jatropha curcas L. In Biofuel and industrial products from Jatropha curcas, eds. G. M. Gubitz, M. Mittelbach, and Trabi. 82-86. Graz: DBV.
Rug M, Ruppel A, (2000). Toxic activities of the plant Jatropha curcas against intermediate snail hosts and larvae of schistosoomes. Tropical Medical International Health 5, 423-430.
Sherchan DP, Thapa YB, Khadka JT, Tiwari TP, (1989). Effect of green manure on rice production. Pakistan journal of Agriculture 2, 12-15.
Thomas R, Debnath M, Devappa RK, He W, King AJ, Ebuehi OA, (2008). Therapeutic biology of Jatropha curcas: a mini review. Trends in Parasitology 25, 151-156.
Ugbomoiko US, Ofoezie IE, Okoye IC, Heukelabach J, (2010). Factors associated with urinary Schistosomiasis in two peri-urban communities in south-western Nigeria. Annals of Tropical Medicine & Parasitology. 104, 409-419.
WHO, (1965). Molluscicide screening and evaluation. Bulletin of the World Health Organization 33, 567- 581.
WHO, (2002). Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. WHO Tech Rep Ser 912, 1-4.
WHO, (2010). Fact sheet February, 2010 Schistosomiasis. Retrieved on October, 28, 2011 from http://www.google.com.ng.