Greener Journal of Epidemiology and Public Health

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Greener Journal of Epidemiology and Public Health, Vol. 4 (3), pp. 061-070, November 2016.

 ISSN: 2354-2381 © 2016 Greener Journals

Research Article

Manuscript Number: 102116188


(DOI: http://doi.org/10.15580/GJEPH.2016.3.102116188)

 

Toxicity and Safety of Khat (Catha edulis) Consumption during Pregnancy using Olive Baboons (Papio anubis) as Experimental Models: A Prospective Randomised Study

 

Emily Muema1*, Peter Kinyanjui2, James Mbaria3, Joseph Nguta4, Sharon Chepkwony5, Joseph Kamau6, Nyamongo Onkoba7 and Atunga Nyachieo8

 

1,3,4Department of Public Health, Pharmacology and Toxicology, University of Nairobi, P.O. Box 19676-00202 KNH, Nairobi Kenya.

2,6,8Department of Biochemistry, School of Medicine, University of Nairobi, P.O Box 30197-00100 Nairobi-Kenya.

5,6,7,8Institute of Primate Research, P.O. Box 24481-00502 Karen, Nairobi-Kenya.


Abstract


Background: The chewing of khat leaves (Catha edulis Forsk) is widely practiced in East Africa and parts of the Middle East, where it forms a deep-rooted social and cultural function. Consumption of Khat is common among men but recently it has extended to women. Biochemical and toxicological effects of Khat on pregnancy and fetal development in lower animals has been noted. Studies on the effect of khat consumption has focused on men thus no data is available on women and especially during pregnancy. This study was conducted to evaluate the effects of khat on liver and kidney functions during pregnancy using olive baboons as experimental models. 

 

Methods: Six pregnant olive baboons were randomly assigned into khat treatment group (n=3) that received 100mls of crude khat at a dose of 5 g/kg body weight/week for 8 weeks and the control group (n=3) received normal saline during the second trimester. Blood pressure, temperature and weight changes were measured weekly. Blood was collected to assay for liver and kidney function tests and liver and kidney tissues collected during necropsy for histopathological examinations.

 

Results: The levels of aminotransferases, urea and creatinine in the khat treated group were significantly elevated compared to those in the control group. There was a decrease of body weight in the dams, fetal birth weight and levels of albumin and sodium in treatment group compared to those in the control group. Kidney and liver tissues of the baboons and their fetuses treated with khat showed necrosis, periportal fibrosis with focal degenerative changes, glomerular degeneration and infiltration with lymphocytes.  Blood pressure was not significantly different between the two groups.

 

Conclusion: The findings show that crude khat may damage the liver and kidneys and modulate the levels of liver enzymes, urea, creatinine and electrolytes essential for liver and kidney functions. Khat is not safe and its use during pregnancy should be discouraged.

 

Key words: Khat; olive baboons; toxicity; pregnancy; liver and kidney function; safety.

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References


Ageely HM (2009). Prevalence of Khat chewing in college and secondary (high) school students of Jazan region, Saudi Arabia. Harm Reduct. J, 6(11):3.

 

Alam Shabbir M, Ahmad Ali Bin Jerah, Gowhar Nabi and Qayyum Husain (2014). Effect of khat (catha edulis) consumption on the functions of liver, kidney and lipid profile in male population of Jazan Region of Kingdom of Saudi Arabia. IJANS, 3(2):9-14.

 

Al-Habori M, Al-Aghbari A, Al-Mamary M, Baker M (2002): Toxicological evaluation of Catha edulis leaves: a long term feeding. J Ethnopharmacol., 83: 209–217 experiment in animals. . 

 

Al-Hashem FH, Bin-Jaliah I, Dallak MA, Nwoye LO, Al-Khateeb M (2011). Khat  (Catha edulis) Extract Increases Oxidative Stress Parameters and Impairs Renal and Hepatic Functions in Rats. Bahrain Medical Bulletin, 33(1):1-9.

 

Al-Mamary M, Al-Habori M, Al-Aghbari AM, Baker MM (2002). Investigation into the toxicological effects of Catha edulis leaves: a short term study in animals. Phytother. Res, 16:127-132.

 

Al-Mehdar A, El-Denshary E, Abdel-Wahha M (2012). Alpha Lipoic Acid and Alpha-Tocopherol Counteract the Oxidative Stress and Liver Damage in Rats Sub-Chronically Treated with Khat (Catha edulis) Extract. Global J. of Pharmacol. 6(2): 94-105.

 

Al-Motarreb A., Baker K. and Broadley KJ. (2002): Khat: pharmacological and medical aspects and its social use in Yemen. Phytother. Res, 16: 403–413. 

 

Al-Zubairi A., Al-Habori M. and Al-Geiry A (2013). Effect of Catha Edulis (khat) Chewing on Plasma Lipid Peroxidation. J Ethnopharmacol., 87(1): 3-9.

 

D’Hooghe TM, Kyama CM, Chai D, Fassbender A, Vodolazkaia A, Bokor A, Mwenda JM (2009). Nonhuman primate models for translational research in endometriosis. J. Hum. Reprod. Sci. 16(2): 152–161.

 

Dhaifalah I, Santavy J (2004). Khat habit and its health effect. A natural amphetamine. Biomedical Papers of the Medical Faculty of University of Palacky Olomouc Czech Republic , 148(1):11–15.

 

Fahaid H,  Al-Hashem, Ismaeel Bin-Jaliah, Mohammad A, Dallak, Luke O Nwoye, Mahmoud Al-Khateeb, Hussein F Sakr, Refaat A Eid, Khalid S Al-Gelban, Hasan S Al-Amri, Mohamed A Adly (2011). Khat (Catha edulis) Extract Increases Oxidative Stress Parameters and Impairs Renal and Hepatic Functions in Rats. Bahrain Medical Bulletin., 33:1-9.

 

Herny JA, Fallon JK, Kicman AT, Hutt AJ, Cowan DA, Forsling M (1998). Low-dose MDMA (“ecstacy”) induces vasopressin secretion. Lancet, 351(9118):1784.

 

Islam MW, al-Shabanah OA, al-Harbi MM and al-Gharably NM (1994): Evaluation of teratogenic potential of khat (Catha edulis Forsk.) in rats. Drug Chem. Toxicol. 17(1): 51–68.

 

Kassim S, Croucher, R (2009). Factors associated with dental and medical care attendance in UK resident YEMENI Khat chewers; a cross sectional study. BMC Public Health, 12: 486.

 

Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG (2013). Improving bioscience research reporting: The arrive guidelines for reporting animal research. Animals, 4:35–44.

 

Mwenda JM., Owuor RA., Kyama CM., Wango EO., M’Arimi M. and Langat DK. (2006): Khat (Catha edulis) up-regulates testosterone and decreases prolactin and cortisol levels in the baboon. J Ethnopharmacol., 103:379-84.

 

Nencini P, Ahmed AM (1989). Khat consumption: a pharmacological review. Drug Alcohol Depend. 23(1):19.

 

Nyachieo A, Chai DC, Deprest J, Mwenda JM, D’Hooghe TM (2007). The baboon as a research model for the study of endometrial biology, uterine receptivity and embryo implantation. Gynecol Obstet Invest. 64: 149–155.

 

Nyachieo A, Kiraithe MM, Spiessens C, Chai DC, Kiulia NM, D’Hooghe TM, Mwenda JM (2013). Short-Term Effects of High-Dose Khat on Sperm Parameters and Reproductive Hormonal Levels in Olive Baboons (Papio  anubis). Gynecol Obstet Invest. 75: 109-114.

 

Pennings EJM, Opperhuizen A, van Amsterdam JGC (2008). Risk assessment of khat use in the Netherlands: A review based on adverse health effects, prevalence, criminal involvement and public order. ‎Regul. Toxicol. Pharm, 52:199–207.

 

Shewamene Z, Engidawork E (2014). Subacute administration of crude khat (Catha edulis F.) extract induces mild to moderate nephrotoxicities in rats. BMC Complement Altern Med. 14:66.

 

Stevens VC (1997). Some reproductive studies in the baboon. Hum Reprod Update, , 3: 533-40.

 

Toennes SW, Harder S, Schramm M, Niess C, Kauert GF (2003). Pharmacokinetics of cathinone, cathine and norephedrine after the chewing of khat leaves. Br. J. Clin. Pharmacol, 56(1): 125–30.

 

Workineh Getahun, Teferi Gedif, FikruTesfaye (2010). Regular Khat (Catha edulis) chewing is associated with elevated diastolic blood pressure among adults in Butajira, Ethiopia: A comparative study. BMC Public Health, 10: 390.