Greener Journal of Epidemiology and Public Health

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Greener Journal of Epidemiology and Public Health, Vol. 5 (5), pp. 050-055, September 2017.

 ISSN: 2354-2381 © 2017 Authors

Research Article

Manuscript Number: 09201731



Consumption of Uncooked Fermented pentaclethramacrophylla Bent (Ugba) and its Bacteriological effects in the Gastrointestinal Tract System of Male Albino Wistar Rats


Oguwike F.N*1, Emenuga V.N2, Ebede S.O3 

Nwobodo H4, Offor C.C, Eluke B.C2.


1Department of Physiology, Faculty of Basic Medical Sciences, Chukwuemeka Odumegwu Ojukwu University Amaku-Awka Anambra State.

2Department of Medical Lab. Sciences, Faculty of Health Sciences and Technology, University of Nigeria Enugu Campus, Enugu State.

3Department of microbiology, University of Nigeria Teaching Hospital Ituku Ozalla Enugu, Enugu State.

4Department of Medical Microbiology Enugu State University of Science and Technology Enugu, Enugu State.


Various reasons have been suggested by concerned researchers why P. macrophyllabenth should be consumed by subjects. This research is geared towards knowing the bacteriological effect of consuming fermented Pentaclethra macrophyllabenth in  the gastrointestinal region of male albino wistar rats.Thirty male albino rats were randomly selected weighing (200-250g) for the study.They were divided into three groups, Group A, B and C each containing 10 rats. Group A served as control group, Group B received uncooked fermented P. macrophyllabenth, rat pellet and water ad libitum, Group C received cooked P.macrophyllabenth,rat pellet and water ad libitum.

Swab samples were collected from the oral cavity and intestinal region of the rats and the percentage of bacteria loads in these sites of gastrointestinal tracts were evaluated. It was observed from the result of the study that of all the microbes identified in fermenting P. macrophyllabenth, such as micrococcus specie, staphylococcus species, bacillus specie, proteus specie, pseudomonas specie and lactobacillus specie, Staphylococcus aureus occurred more in percentages (70%) in the intestinal regions of rats fed with uncooked fermented P.macrophyllabenth and also in the intestinal region  of those fed with cooked fermented P. macrophyllabenth (40%). Bacillus species occurred more in the oral cavity of rats fed with uncooked. P. macrophyllabenth (80%), followed by Staphylococcus aureus (60%). If virulent bacteria eventually escape digestive enzymes and heat in the gut system can get to the urinary system during defecation causing infection of the urinary system.


Key Words: Bacteria, gastrointestinal tract, P. macrophyllabenth ,wistar rats.

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