Greener Journal of Microbiology and Antimicrobials

Greener Journal of Microbiology and Antimicrobials Vol. 2 (1), pp. 007-014, March 2014.

 ISSN: 2354-2284  

Research Paper

Manuscript Number: 010814023

 

The use of pAL5000 Replicon Vectors to Transform Rhodococcus and GordoniaSpecies

 

M.D. Chengalroyen¹* and E.R. Dabbs²

 

¹Department of Genetics, University of Witwatersrand, Johannesburg, Postal address:

P.O. Box 1038, South Africa.

²Department of Genetics, University of Witwatersrand, Johannesburg, South Africa.

 

Email: ²Eric.Dabbs @ wits. ac. za

 

*Corresponding Author’s Email: mchengalroyen @ gmail. com, Tel: 0114899134

Abstract:

pAL5000 based vectors, conventionally used to transform Mycobacterium species were used in this study to transform Rhodococcus and Gordonia species. Adequatetransformation efficiencies were obtained ranging between 4.5x10³ – 2.8x10⁶.Additionally, a pAL5000 based vector with suicide function was generated. This vectorwas transformed into Rhodococcus, Mycobacterium and Gordonia species and was maintained as a stable construct.

Keywords: pAL5000, Rhodococcus, Gordonia, suicide vector.

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