Chengalroyen and Dabbs
Greener Journal of Microbiology and Antimicrobials Vol. 2 (1), pp. 007-014, March 2014.
ISSN: 2354-2284 © 2013 Greener Journals
Manuscript Number: 010814023
The use of pAL5000 Replicon Vectors to Transform Rhodococcus and GordoniaSpecies
M.D. Chengalroyen1* and E.R. Dabbs2
1Department of Genetics, University of Witwatersrand, Johannesburg,
P.O. Box 1038, South Africa.
2Department of Genetics, University of Witwatersrand, Johannesburg, South Africa.
Email: 2Eric.Dabbs @ wits. ac. za
*Corresponding Author’s Email: mchengalroyen @ gmail. com
pAL5000 based vectors, conventionally used to transform Mycobacterium species were used in this study to transform Rhodococcus and Gordonia species. Adequatetransformation efficiencies were obtained ranging between 4.5x103 – 2.8x106.Additionally, a pAL5000 based vector with suicide function was generated. This vectorwas transformed into Rhodococcus, Mycobacterium and Gordonia species and was maintained as a stable construct.
Keywords: pAL5000, Rhodococcus, Gordonia, suicide vector.