Greener Journal of Microbiology and Antimicrobials

Open Access

Samaila et al

Greener Journal of Microbiology and Antimicrobials Vol. 3 (1), pp. 001-007, March 2017.

 ISSN: 2354-2284 © 2017 Greener Journals

Research Paper

Manuscript Number: 012517009


(DOI: http://doi.org/10.15580/GJMA.2017.1.012517009)

 

Antimicrobial Activity of the Leaf and Root Extracts of Kigelia africana and Albizia chevalieri against Staphylococcus aureus

 

Samaila A.B.1*, Yarma A.A.2, Oshomoh E.O.3 and Gabriel M.1

 

1Department of Biological Sciences, Abubakar Tafawa Balewa University, PMB 0248, Bauchi State, Nigeria.

2Yarma Memorial Hospital Gombe, Gombe State, Nigeria.

3Department of Science Laboratory Technology, Faculty of Life Sciences, University of Benin, Edo State, Nigeria.


Abstract


The investigation was carried out to determine the antimicrobial activity of the Leaf and Root extracts of Kigelia africana (nonon giwa) and Albizia chevalieri (katsari) against Staphylococcus aureus. Antimicrobial activity was evaluated by disc diffusion method and MIC and MBC were calculated by double fold dilution method. The antimicrobial activity result shows 20mm zone of inhibition Kigelia africana leaf at 100% concentration and 16mm zone of inhibition for albezia chevalieri leaf at same concentration while for the roots 14mm and 11mm zone of inhibition at 100% concentration. The maximum inhibition zone is found at 20mm and minimum inhibition zone at 11mm. Also the antimicrobial result shows the MIC of 6.25mg/ml against Staphylococcus aureus and MBC of 12.5mg/ml and 10.5mg/ml for both extracts. The study reveals that the extracts possess antimicrobial activity in a dose dependent manner. This antimicrobial activity may be due to the presence of some active compounds, further studies are highly needed for the drug development.

 

Keywords: Antimicrobial, Leaf, Root extracts, Kigelia Africana, Albizia chevalieri, Staphylococcus aureus.

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References


Ahmed, M., Djebli, N., Meslem, A. and  Aisshat, S (2012). Antibacterial activity of various honey types of Algeria against Pathogenic Gram-Negative Bacilli: Escherichia coli and Pseudomonas aeruginosa. Asian Pac. J. Trop. Dis.; (3): 211-214.

 

Akunyili, D.N., Houghton, P.J. and Raman A (1991) Antimicrobial activities of the stem bark of Kigelia pinnata. J. Ethnopharm; 35: 173–177.

 

Ankit, G., Madhu, N. and Vijay, K (2012): Extraction Methods for the Preparation of Bioactive Plant Extracts. Intl. J. Appl. Nat. Sci.; 1(1):8-26.

 

Azuine, M.A., Ibrahim, K., Enwerem, N.M., Wambebe, C. and Kolodziej, H (1997) Protective role of Kigelia africana fruits against benzo[a]pyrene-induced for stomach tumourigenesis in mice and against albumen-induced inflammation in rats. Pharmaceutical and Pharmacological Letters 7: 67–70.

 

Binutu, O.A., Adesogan, K.E. and Okogun, J.I (1996) Antibacterial and antifungal compounds from Kigelia pinnata. Planta Medica 62: 352–253.

 

Burkill, H.M. (1995). The Useful Plants of West Tropical Africa Vol. 3. Families J-L, Royal Botanical Gardens, Kew. Pp. 207-208.

 

Constantine, D.S (2007). Extraction, Separation and Detection Methods for Phenolic acids and Flavonoids. J. Sep. Sci. 30:3268-3295.

 

Ekeanyanmu, C.R (2011). Traditional Medicine in Nigeria; Current Status and the Future. Res. J.  Pharm.; 5(6):90-91

 

Eloff, J.N (1998). Which extract should be used for the screening and isolation of antimicrobial compounds from plants. J. Ethnopharm. 60:1-8.

 

Gracelin, D.H.S., De-Britto, A.J., Jeya, P.B. and Kumar, R (2013). Qualitative and Quantitative Analysis of Phytochemicals in Five Pteris species. Intl. J. Pharm. and Pharmacl. Sci.; 5(1):105-107

 

Hassan, M.M., Oyewale, A.O., Amupitan, J.O., Abduallahi, M.S. and Okonkwo, E.M. (2004). Preliminary phytochemical and antibacterial investigation of crude extracts of the root bark of Detarium microcarpum. J. Chem. Soc. Nigeria.; 29: 26-29.

 

Hemendra, S.C. and Sushil, K.S (2010). Antibacterial Activity of Seed and Flower Parts of Crotalaria juncea Linn. Am.-Eurasian J. Sci. Res. 5(3):212-215

 

Igidi, O.J. and Edene, C.E (2014). Proximate and phytochemical compositions of Napoleona vogelii hook fruit.  Intl. J. Engr. Sci.; 6:46-51

 

Jeyachandran, R. and  Mahesh, A (2007). “Antimicrobial evaluation of Kigelia africana (Lam).” Res. J. Microbiol., vol. 2, pp. 645-649.

 

Karuppannan, K., Subramanian, D.P. and Venugopa, S (2013): Phytopharmacological Properties of Albizia Species. Intl. J. Pharm. Pharmacal. Sci. 5(3):70-73

 

Khan, M.R (1998) Cytotoxicity assay of some Bignoniaceae. Fitoterapia 69: 538–540

 

Kwo, V.T. and Craker, L.E (1996) Screening Cameroon medicinal plant extracts for antimicrobial activity. Acta Horticulturae 426: 147–155.

 

Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G (2005). Determination of the total phenolic, flavonoid  roline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem.; 91: 571-577.

 

Nascimento, G.G.F., Lacatelli, J., Freitas, P.C. and Silva, G.L (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz. J. Microbiol. 31(4): 886-891.

 

National Committee for Clinical Laboratory Standard (1990). Methods for the antimicrobial susceptibility testing. In: Manual of Clinical Microbiology. Am. Soc. Microbiol. Washington, DC. 5th edition, pp. 1105-1125.

 

Owolabi, .J. Omogbai, E.K.I.and Obasuyi, O (2007). Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigelia africana (Bignoniaceae) stem bark. Afr. J. Biotechnol. 6 (14): 882-85.

 

Prashant, T., Bimlesh, K., Mandeep, K., Gurpreet, K. and Harleen, K (2011): Phytochemical Screening and Extraction; A Review. Internationale Pharmaceutica Sciencia, 1(1):98-106

 

Retief, E. and Herman, P.P. J (1997) Plants of the Northern Provinces of South Africa: Keys and Diagnostic Characters. National Botanical Institute, Pretoria, South Africa. ISBN 874907–30–7

 

Saha, A. and Ahmed, M (2009). The analgesic and anti-inflammatory activities of the extract of Albizia lebbeck in animal model. Pak. .J Pharm. Sci.; 22(1): 74-77.

 

Sikder, M.A.A.A., Hossain, A.K.M.N.,  Kaisar, M.A., and Rashid, M.A (2011).“in vitro anti-microbial screening of four reputed medicinal plants.” Pharmaco. J., vol.3, 2011, pp.72-76.

 

Tempone, A.G., Sartorelli, P., Teixeira, D., Prado, F.O. Calixto, I.A.R.L., Lorenzi, H. and Melhem, M.S.C. (2008). Brazilian flora extracts as source of novel antileishmanial and antifungal compounds, Mem. Inst. Oswaldo Cruz, 103(5): 443-449.

 

Usman, H. and Osuji, J.C. (2007). Phytochemical and in vitro anti-microbial assay of the leaf extract of New bouldia leavis. Afr. J. Trad. Med. 4: 476-480.

 

Yusuf, S., Lawal, S.B., Mansur, L., Simeon, A.I. and Rabiu, A.U. (2007): Hematotoxicity study of the leaf extract of Albizia chevalieri (Harms) (leguminoseae). Biochemia medica, 17(2):203-211.