Waithaka et al
Greener Journal of Microbiology and Antimicrobials Vol. 3 (2), pp. 008-017, May 2017.
Manuscript Number: 041817051
Isolation of Actinomycetes from the Soils of Menengai Crater in Kenya
Paul Njenga Waithaka¹*, Francis B. Mwaura¹, John M. Wagacha¹ and Eliud M. Gathuru²
1University of Nairobi, School of Biological Sciences, P. O. Box, 30197-00100 Nairobi, Kenya.
2Egerton University, Department of Biological Sciences, P. O. Box, 536 Njoro, Kenya.
This study was carried out to isolate actinomycetes from the soils of Menengai crater. The study area was divided into regions A, B, C and D. Soil samples were collected from 8 sampling points from each region. The samples were separately mixed to form composite samples. Starch casein, Luria Bertani and starch nitrate agar were used in isolating actinomycetes using spread plate technique. Prior to isolation, the soil samples were heat for 1h at 121oC followed by serial dilution upto 10-6. The isolates were characterized using cultural, morphological and biochemical means. Region B and C produced many actinomycetes than A and D. Luria Bertani agar was better in recovering actinomycetes than starch casein and starch nitrate agar. There was significant difference in the number of actinomycetes isolated using the three media (F=3.315 P=0.04218). The number of actinomycetes isolated from region A, B C, and D also varied significantly (F= 27.50 P=0.000). A total of 152 actinomycetes were isolated. There is need to test the actinomycetes isolates for production of antimicrobials.
Keywords: actinomycetes, isolation, Kenya, Luria Bertani, Menengai, Starch casein.
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