Waithaka et al
Greener Journal of Microbiology and Antimicrobials Vol. 3 (2), pp. 008-017, May 2017.
ISSN: 2354-2284 © 2017 Greener Journals
Manuscript Number: 041817051
Isolation of Actinomycetes from the Soils of Menengai Crater in Kenya
Paul Njenga Waithaka¹*, Francis B. Mwaura¹, John M. Wagacha¹ and Eliud M. Gathuru²
1University of Nairobi, School of Biological Sciences, P. O. Box, 30197-00100 Nairobi, Kenya.
2Egerton University, Department of Biological Sciences, P. O. Box, 536 Njoro, Kenya.
This study was carried out to isolate actinomycetes from the soils of Menengai crater. The study area was divided into regions A, B, C and D. Soil samples were collected from 8 sampling points from each region. The samples were separately mixed to form composite samples. Starch casein, Luria Bertani and starch nitrate agar were used in isolating actinomycetes using spread plate technique. Prior to isolation, the soil samples were heat for 1h at 121oC followed by serial dilution upto 10-6. The isolates were characterized using cultural, morphological and biochemical means. Region B and C produced many actinomycetes than A and D. Luria Bertani agar was better in recovering actinomycetes than starch casein and starch nitrate agar. There was significant difference in the number of actinomycetes isolated using the three media (F=3.315 P=0.04218). The number of actinomycetes isolated from region A, B C, and D also varied significantly (F= 27.50 P=0.000). A total of 152 actinomycetes were isolated. There is need to test the actinomycetes isolates for production of antimicrobials.
Keywords: actinomycetes, isolation, Kenya, Luria Bertani, Menengai, Starch casein.
Ajunwa, O. M., Audu, J. O., Adedeji, B. A., and Ja’afaru, M. I. (2016). Assessment of in vitro effects of chlorpyrifos on biomass and antimicrobial production functionality of Actinomycetes isolated from soils in Yola, Nigeria. New York Science Journal; 9(6:52-57.
Arya, G. and Singh, G. (2016). Statistical analysis of actinomycetes isolated from the Haridwar region of Uttarakhand (India). Journal of drug delivery and therapeutics; 6(4):31-36.
Ashokvardhan, T. and Satyaprasad , K. (2016). Antibacterial activity of actinomycetes isolated from Capsicum annuum L. International journal of pharma and bio sciences; 7(1): 397 – 402.
Atsuko, M. and Takahashi, T. 2017.Endophytic actinomycetes: promising source of novelbioactive compounds. The journal of antibiotics;3:1–6.
Atta, H. M. (2015).Biochemical studies on antibiotic production fromStreptomyces sp.: Taxonomy, fermentation,isolation and biological properties.Journal of Saudi Chemical Society; 19:12–22.
Basha, C. S. and Sambasiva, K. R. S. (2017). An intrinsic assessment of bioactive potentiality of mangroves actinomycetes. Journal of entomology and zoology studies; 5(1): 20-26.
Cassir, N., Rolain, J. M. and Brouqui, P. (2014). "A new strategy; to fight antimicrobial resistance: the revival of old antibiotics.". Frontiers in microbiology;5: 551-560.
Chavan, V., Mulaje S. S. and Mohalkar R. Y. (2013). A review on actinomycetes and their biotechnological application.International journal of pharmaceutical sciences and research; 4(5): 1730-1742.
Cholarajan, A. and Vijayakumar, R. (2016). screening of antibiotic (Staurosporine) producing actinobacteria (Streptomyces sp.) from terrestrial environment soil of Thanjavur District, Tamilnadu, India.European journal of biomedical and pharmaceutical sciences; 3(3):480-493.
Chavan, V., Mulaje S. S., Mohalkar R.Y. (2013). A review on actinomycetes and their biotechnological application.International Journal of pharmaceutical sciences and research, 4(5): 1730-1742.
Duddu, M. K. and Guntuku, G. (2016). Isolation, screening and characterization of antibiotic producing actinomycetes from Kapuluppada plastic waste dumping yard, Visakhapatnam.International journal of pharmacy and pharmaceutical sciences; 8 (11): 1-9.
Humera, M., Tumane, P. M. and Durgesh, D. W. (2016). Isolation and Characterization of Anti-Mrsa Producing Actinomycetes from Soil. International journal of recent science research;7(10): 13564-13568.
Ibrahim, S. Y. and Abd El-Salam, M. M (2016). Isolation of anti-fungal agent from a soil inhabitant Streptomyces albaduncus-M51 and its efficacy against osmophilic food spoilage by Saccharomyces cerevisiae. Journal of environmental and occupational science; 5(2): 38-46.
Janaki, T., Nayak, B. K. and Ganesan, T. (2016).Antibacterial activity of soil actinomycetes from themangrove Avicennia marina. Journal of pharmacognosy and phytochemistry; 5(1): 267-271.
José, V. L., Suzana, C. S., Katia, A. S., Marcos, A. S. and Claudia, M. M. (2017). Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia. African journal of biotechnology; 16(11):499-507.
Khasabuli, O. Y. and Kibera, A. N. (2014). Isolation, characterization and primary screening of soil actinomycetes from Kenyatta University arboretum grounds for antibacterial activities. Journal of applied biosciences; 74:6072– 6079.
Kumari, M., Menghani, E.and Mithal, R.(2016).Actinomycetes as Antibiotic Agents: Future generation Bio-factory. International journal of scientific and engineering research; 7(3): 1286- 1296
Manojkumar, S., and Subbaiya, R. (2016). Isolation and Identification of Soil derived Actinomycetes Nocardiopsis alba. Research journal of pharmaceutical, biological and chemical sciences; 7(3): 485-493.
Memon, H., Tumane, P. and Wasnik, D. (2016). Isolation and characterization of anti-MRA producing actinomycetes from soil. International journal of recent scientific research; 7(10): 13564-13568.
Modi, S., Chauhan, B., Gahlout, M and Prajapati, H. (2016). Extraction and isolation of melanin from actinomycetes. International journal of drug research and technology; 6(2): 79-86.
Pooja, P., Lekshmi, M. and Jayadev, A. (2016).Isolation and Characterization of Actinomycetes from Mangrove Samples. International journal of science research; 2(9): 1-10.
Prashith, M., Pondet, K., and Kwantep, K. (2015).In vitro antimicrobial and antioxidant activities of bioactivecompounds (secondary metabolites) extracted from Streptomyceslydicus A2. Science, Technology and arts research journal;3(4): 116-121.
Qais, M. A., Maher, A., Ola, A. A. and Abdullah, Y. A. (2017).New Records of Streptomyces and Non Streptomyces Actinomycetes Isolated from Soils Surrounding Sana'a High Mountain. International journal of research in pharmacy and biosciences; 3(3): 19-31.
Rajan, B. M. and Kannabiran, K. (2014). Extraction and identification of antibacterial secondary metabolites from marine Streptomyces sp. VITBRK2. International journal of molecular cell medicine; 3(3): 130-137.
Rotich, M., Magiri, E., Bii, C. and Naomi, M. (2017). Bio-Prospecting for Broad Spectrum Antibiotic Producing Actinomycetes Isolated from Virgin Soils in Kericho County, Kenya. Advances in microbiology; 7:56-70.
Saravana, K., Duraipandiyan, V. and Ignacimuthu, S. (2014). Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7. Kaohsiung journal of medical sciences; 30, 435-446.,
Siddique, S., Syed, Q., Ahmad, A. and Qureshi, F. A. (2014). Isolation, Characterization and Selection of Avermectin-Producing Streptomyces avermitilis Strains from soil samples. Jundishapur journal microbiology. 7(6): e10366.
Slam, M. S., Aktar, M. B., Rahman, M. M. and Main, K. M. (2014). Isolation and characterization of Streptomyces spp collected from Bangladeshi soils on the basis of morphological and biochemical studies.International Journal of Current Microbiology and Applied Sciences; 3(11): 734-742.
Surabhi, T. and Srividya, S. (2016).Lytic actinomycete based extraction of Polyhydroxyalkanoates (PHAs) from Bacillus megaterium Ti3. International journal of advanced life sciences; 9(2):227-233.
Tebo, B. M., Davis, R. E., Anitori, R. P., Connell, L. B., Schiffman, P. and H. Staudigel, H. (2015). Microbial communities in dark oligotrophic volcanic ice cave ecosystems of Mt. Erebus, Antarctica. Frontiers of Microbiology; 6:1–14.
Varalakshmi, T., Sekhar, K and Charyulu, P. B. (2014). Taxonomic studies and phylogenetic characterization of potential and pigmented antibiotic producing actinomycetes isolated from rhizosphere soils. International journal of pharmacy and pharmaceutical sciences; 6 (6): 511-519.
Wadetwar, R. N. and Patil, A. T. (2013). Isolation and characterization of Bioactive Actinomycetes from soil in and around Nagpur. International journal of pharmaceutical sciences and research;4(4): 1428-1433.
Wang, X., X. Wang, W. Zhang, et al. 2016. Invariant community structure of soil bacteria in subtropical coniferous and broadleaved forests. Scientific reports; 6:19071.
Yabe, S., Sakai, Y., Abe1, K. and Yokota, A. (2017). Diversity of Ktedonobacteria with actinomycetes-like morphology in terrestrialenvironments. Microbes environments; 32:(1) 61-70.
Zakalyukina, Y., Biryukov, M. V., Golichenkov,M.V. and Netrusov, A. I. (2017). Phenotypic and Phylogenetic Characterization of actinomycetesisolated from Lasius niger and Formica cunicularia Ants. Moscow University biological sciences bulletin, 72(1) 13–19.
Zhang, X., Chongxi, L., Yuejing, Z., Haiya, W., Shurui, W. Xiangjing, W. Wensheng, X. (2014). Erratum to: Streptosporangium shengliensis sp. nov., a novel actinomycete isolated from a lake sediment. Antonie van Leeuwenhoek; 105:265