Greener Journal of Microbiology and Antimicrobials

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Rabi’u et al

Greener Journal of Microbiology and Antimicrobials Vol. 3 (3), pp. 022-026, August 2017.

 ISSN: 2354-2284 

Research Paper

Manuscript Number: 042717055



Isolation and Antibacterial Activity of Antibiotic-Producing Bacteria Recovered from Soil in Kano, Nigeria


*1Rabi’u M.A., 2Kawo A.H., *3Abdullahi S.A., 2Bala I., 3Umar F.A.,

1Chamo A.M., 1Sabo B.B. and 1Karaye A.K.


1Jigawa Research Institute Kazaure, Jigawa State, Nigeria

2Department of Microbiology Bayero University, Kano, Nigeria

3Public Health and Diagnostic Institute, Northwest University Kano, Nigeria


Filamentous soil bacteria belonging to the genus Streptomyces are widely recognized as industrially important micro-organisms for their ability to produce secondary metabolites including antibiotics. The present study aimed at isolation and determination of antibacterial activity of antibiotic producing bacteria recovered from soil in Kano. Forty eight different soil samples were collected from Gwarzo, Katsina, Hadejia and Maiduguri roads. Twelve soil samples were collected from each of the four different sites and transported to Postgraduate Laboratory, Bayero University Kano for physical and microbiological analysis. The mean value of soil temperature, pH, moisture content and organic matter obtained are 26-270C, 6.5-7.5, 43.4-46.9%and 18.9-19.8% respectively whereas the mean Streptomycetal count obtained is 1.46x106-1.53x6cfu/g. The Streptomyces species isolated   include: Actinomadura, Nocardia, Actinomyces and Streptomyces and were tested against clinical bacterial isolates of Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa and Escherichia coli. The metabolites extracted and tested were found to be of broad spectrum that inhibited the growth of both Gram positive and Gram negative bacteria with zones of inhibition up to 31mm. Thin layer chromatography of the extracts ranged from 0.67-0.78 Retention Factor and infrared spectrum revealed how much functional groups were absorbed at each transmittance, the position, shape and intensity of the peaks were analyzed in the spectrum. The investigation demonstrate that strains of antibiotic-producing bacteria are present in the soil of Kano Metropolis, Nigeria and these could be harnessed by the pharmaceutical industries for the production of antibiotics from local sources.


Keywords: Isolation; Antibacterial activity; Streptomyces; Soil.

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Alanis A.J (2005) Resistant to antibiotics, Archives of Medical Research 36; 697 – 705


Bahing (2008) Isolation, characterization and application of bacterial population from Agricultural soil at Sohang Province, Egypt. Malaysian Journal of Microbiology 4(2): 42-50.                


Borodina Irina, Preben Krabben and Jens Nielsen. (2005). “Genome-scale Analysis of streptomyces coelicolorA3[2]; Pp. 820-828.


Cheesbrough M. (2010) District Laboratory Practice in Tropical Countries 2nd edition (2) 64 – 70 Cambridge University Press United Kingdom.


Gliessman (1998). Field and Laboratory Investigations in Agroecology University of California, Santacruz Lames Publishers, USA  


Liu, P.L., Lau, Y.J., Hu, B.S., Shyr, J.M., Shi, Z.Y., Tsai, W.S., Lin, Y.H. and Tseng, C.Y. (1995); comparison of susceptibility to extended spectrum B-lactam antibiotics and Ciprofloxacin among gram-negative bacilli isolated from intensive care units. Diagn Microbiol Infect. 22(3): 285 – 291.


Makut M.D and Owolewa O.A. (2011) Antibiotic-producing Fungi Present in the Soil Environment of Keffi Metropolis, Nasarawa State, Nigeria Trakia Journal of Sciences, . 9, (2) 33-39


Miyadoh, S. (1993). Research on antibiotic Screening in Japan over the Last Decade. A Producing Micro- Organisms approach. Actinomycetologica (9)100-106.


Paula, Peter Atkins, Julio De, (2009) Elementary Physical Chemistry (5th edition) Pp. 459.


Rana S., Salam MD (2014). Antimicrobial potential of Actinomycetes isolated from soil samples of Punjab. India J Microbiol Exp 1(2)00010


Satvinder Kaur, Jatinder Kaur, *Pranay P Pankaj(2014)Isolation and Characterization of Antibiotic Producing Microorganisms from Soil Samples of Certain Area of Punjab Region of India International Journal of Pharmaceutical and Clinical Research 2014; 6(4): 312-315


Sihem BM, Rafik E, Florence M. Muhammad C, Ahmed L (2001) identification and partial characterization of antifungal and antibacterial activities of two Bacillus specie strains isolated from salt soil in Tunisia Afr. Microbial Res (5)13:  1599 – 1608.


Stackebrandt, E.D.,Witt,C.,Kemmerling, M. and Liesac, W. (1991);. Designation of Streptomycete165 and 235 rRNA based target regions of ohgomocleotide probes. Applied Environ Microbiol 57: 1468-1477.

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