Rabi’u et al
Greener Journal of Microbiology and Antimicrobials Vol. 3 (3), pp. 022-026, August 2017.
Manuscript Number: 042717055
Isolation and Antibacterial Activity of Antibiotic-Producing Bacteria Recovered from Soil in Kano, Nigeria
*1Rabi’u M.A., 2Kawo A.H., *3Abdullahi S.A., 2Bala I., 3Umar F.A.,
1Chamo A.M., 1Sabo B.B. and 1Karaye A.K.
1Jigawa Research Institute Kazaure, Jigawa State, Nigeria
2Department of Microbiology Bayero University, Kano, Nigeria
3Public Health and Diagnostic Institute, Northwest University Kano, Nigeria
Filamentous soil bacteria belonging to the genus Streptomyces are widely recognized as industrially important micro-organisms for their ability to produce secondary metabolites including antibiotics. The present study aimed at isolation and determination of antibacterial activity of antibiotic producing bacteria recovered from soil in Kano. Forty eight different soil samples were collected from Gwarzo, Katsina, Hadejia and Maiduguri roads. Twelve soil samples were collected from each of the four different sites and transported to Postgraduate Laboratory, Bayero University Kano for physical and microbiological analysis. The mean value of soil temperature, pH, moisture content and organic matter obtained are 26-270C, 6.5-7.5, 43.4-46.9%and 18.9-19.8% respectively whereas the mean Streptomycetal count obtained is 1.46x106-1.53x6cfu/g. The Streptomyces species isolated include: Actinomadura, Nocardia, Actinomyces and Streptomyces and were tested against clinical bacterial isolates of Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Proteus mirabilis, Pseudomonas aeruginosa and Escherichia coli. The metabolites extracted and tested were found to be of broad spectrum that inhibited the growth of both Gram positive and Gram negative bacteria with zones of inhibition up to 31mm. Thin layer chromatography of the extracts ranged from 0.67-0.78 Retention Factor and infrared spectrum revealed how much functional groups were absorbed at each transmittance, the position, shape and intensity of the peaks were analyzed in the spectrum. The investigation demonstrate that strains of antibiotic-producing bacteria are present in the soil of Kano Metropolis, Nigeria and these could be harnessed by the pharmaceutical industries for the production of antibiotics from local sources.
Keywords: Isolation; Antibacterial activity; Streptomyces; Soil.
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