Amonkan et al
Greener Journal of Medical Sciences Vol. 3 (4), pp. 129-134, May 2013.
ISSN: 2276-7797 © 2011 Greener Journals
Manuscript Number: 022213488
Comparative Effects of Two Fractions ofPhyllanthus amarus (Euphorbiaceae) on the Blood Pressure in Rabbit
1Augustin K. Amonkan, 2Mamadou Kamagaté, 3Alain N.R. Yao, 4*André B. Konan, 5Mathieu N. Kouamé,6Camille Koffi,
7Séraphin Kati-Coulibaly, 8Henri Die-Kakou
1,4,7Laboratoire de Nutrition et Pharmacologie, UFR Biosciences, Université Félix Houphouët Boigny,
22 BP 582 Abidjan 22, Côte d’Ivoire.
2,3,6,8Service de Pharmacologie, UFR-SMA, Université Félix Houphouët-Boigny, Côte d’Ivoire.
4*Corresponding Author’s Email: akonanb @ yahoo. fr
Phyllanthus amarus is a species used to treat many ailments. The hypotensive effect reported by the pharmacopoeia has been demonstrated for the aqueous extract. This effect is reduced in the presence of atropine. The aim of this work is to evaluate two fractions of P. amarus aqueous extract (EAPA), the aqueous fraction (FAPA) and ethanolic fraction (FEPA), on blood pressure. Phytochemical screening shows that EAPA and FEPA differs by the absence of saponins, while EAPA and FAPA differs by the absence of alkaloids. The aqueous extract and fractions both lower blood pressure in a dose-dependent. The ED50 obtained were 25.77 ± 2.76 mg/kg b.w. (EAPA), 22.26 ± 2.59 mg/kg b.w. (FAPA) and 13.41 ± 1.63 mg/kg b.w. (FEPA). Hypotension induced by FEPA is also reduced in the presence of atropine. FEPA does not alter the hypertension induced by adrenaline. Ethanolic fraction of P. amarus has a hypotensive effect that is relatively large. These effects result mainly from its cholinergic action of chemical compounds present in this fraction.
Keywords: Phyllanthus amarus, Hypotension, Aqueous fraction, Ethanolic fraction.