Chakir et al
Greener Journal of Medical Sciences Vol. 3 (4), pp. 111-120, May 2013.
ISSN: 2276-7797 © 2011 Greener Journals
Manuscript Number: 032713548
Effects of Vitamin E and Vitamin C on Hydrogen
Peroxide-Induced Hemolysis in Moroccan Dromedary Camels (Camelus Dromedarius)
Chakir Youssef1, EL Khasmi Mohammed1*, Farh Mohamed1, Bargaâ Rita1, Riad Fouad1, Safwate Abdallah1, Tahri El Hassan1, El Abbadi Najia2, Abouhafs Rachid3, Faye Bernard4
1Laboratory of Physiopathology and Molecular Genetics, Faculty of Sciences Ben M’Sik,
University Hassan II-Mohammedia, Casablanca, Morocco.
2Unit of Applied Radioimmunology, National Center for Energy Sciences and Techniques and Nuclear Techniques, Maamoura, Morocco.
3Prefectural Veterinary Service of Casablanca, Morocco.
4International Center for Agronomic Research for Development-Environments and Societies (CIRAD-ES). International Campus of Baillarguet, 34398, Montpellier, Cedex 5, France.
*Corresponding Author’s Email: elkhasmi_mohammed @ hotmail. com
Antioxidant vitamins may affect an organism’s capacity for defense against damage caused by reactive oxygen species (ROS), and biological markers of the dietary exposure to these compounds is of importance. The present study was designed to evaluate the protective effect of vitamin E and vitamin C against the hemolysis induced by hydrogen peroxide (H2O2) in camel’s red blood cells in vitro, and forassessing the vulnerability of camel erythrocytes to oxidative stress (OS). Vitamin E and vitamin C were able to protect the red blood cells against the H2O2-induced hemolysis and thiobarbituric acid reactive species (TBARS) formation. The antioxidative property of these vitamins in reducing oxidative injury showed in this work, may suggest that these molecules may be very helpful to fight the ROS during OS induced by transportation, heat, deshydratation, aging, and parasite, infectious and metabolic diseases in dromedary camels.
Keywords: Ascorbic acid; Camel’s red blood cells; Hemolysis; Hydrogen peroxide; Oxidative stress; Vitamin E.