Greener Journal of Medical Sciences

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Oguwike et al

Greener Journal of  Medical Sciences Vol. 6 (3), pp. 046-051, June 2016.

 ISSN: 2276-7797 © 2015 Greener Journals

Research Paper

Manuscript Number: 052016094


(DOI: http://doi.org/10.15580/GJMS.2016.3.052016094)

 

Activities of Coffee in the Fertility Hormones, Haemostatic and Biochemical Profile of Female Albino Wistar Rats

 

*Oguwike FN, Chukwu CL, Oraegbunam SC, Eze RI

 

Dept of Physiology, Faculty of Basic Medical Sciences Chukwuemeka Odumegwu Ojukwu University, Anambra State Nigeria.

Dept of Pharmacology Chukwuemeka Odumegwu Ojukwu University Teaching Hospital Amaku Awka Anambra State Nigeria.

Dept of Medical Lab Science Madonna University Elele Rivers State Nigeria.


Abstract


The activities of Coffee on the fertility hormones, haemostatic and biochemical profile of female Albino wistar rats have been investigated. The rats (180 -200g) were housed in the animal house of the Dept. of physiology of the University. The rats were divided into two groups of 12 rats (control) and 24 rats [Test].Group 1[control]was fed with normal rat feed and tap water ad libitum while group 2(Test rats) were given rat feed Coffee extract and tap water ad libitum. The study lasted for 28days.At the end of the experiment, cardiac puncture was used to collect blood samples for hormonal assay, and blood analysis. Results showed that fertility hormones (FSH, LH, Prolactin, Progesterone, Testosterone) and haematological indices in the test animals were significantly reduced (p<0.05) compared to the control rats. The extract also prolonged the bleeding time, clotting time, Prothrombin time and Partial thromboplastin kaolin test (p<0.05), but most of the liver function profile were not significantly affected except for alkaline phosphatise which was reduced. Therefore it is concluded that Coffee extract affects fertility hormones in rats but does not affect the liver function profiles and protein level, hence suggesting a continuum of favourable effect on liver function and protein concentration of the body.

 

Keywords: Caffeine, fertility hormones, bleeding time, Prothrombin time, Liver enzymes, haemostasis, Haemoglobin.


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References


Aloy-AmadiOluchiChinwe, NnodimJohnkennedy, Okorie Hope, Ogbuokiri Constance and Uduji Helen, (2013). The effects of coffee consumption on liver enzymes and bilirubin in healthy subjects. Journal of current research in science Vol. 1 No 2 Pp. 104.

 

Arthur G.C, (2007). Haemostatis and blood coagulation. Textbook of Medical Physiology p 79-80. Published by W$B Saunders Company, Philadelphia, London, Toronto.

 

Degera E, Villa S, De Gactano G.(1982). Bleeding time in rats. A comparison of different experimental conditions. Thrombi-Haemostat 48:108-111

 

Harbourne J.B.C (1973). Phytochemical method. Chapman and Hall, London p269.

 

Leeeland White W, (1985). Investigation for haemostatic abnormalities. Introduction to Med. Lab. Technology 6th Edition. Bulterworth Publication Woburn p. 346 

 

LorkeD, (1983). A new approach to practical Acute Toxicity Testing Arch. Toxicol 54: 275-278.

 

Oguwike F.N, Ebede S, Offor C.C, Nwafia W.C, Akpuaka F.C (2014). Efficacy of aqueous leaf extract of mangiferaindica(mango) haematological and biochemical profile of induced male diabetic wistar rats. Tropical Journal of Applied Natural Sciences Vol. 1 No. 1 Pg. 173-182.

 

Olatunde, Sarah Haruna, Sambo Ahmed and Luka Carol Domkat (2014). Anti-diabetic activity of aqueous extract of Anacardiumoccidentale (Linn) stem bark in normal and alloxan- induced diabetic albino rats. Journal of Biological and Biocenser Vol.6 No. 2 Pg. 41-57.

 

Quicks W. (1985). Basic haematological technique in practical haematology 6thedition. Churchill Living Stone Edinburg London Melbourne p43.

 

Taofeeq O, Oyeniyi M.A, Tesleem A.O (2005). Mechanisms of actions of Jathrophagossipifolia stem latex as a haemostic agent. European Journal of General Medicine 2(4): 14-145.

 

Trease G.E and Evans W.C (1996). Textbook of pharmacognosy, 14th Edition. W.B. SSaunders London p11.

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