Ughachukwu et al
Greener Journal of Medical Sciences Vol. 7 (2), pp. 022-031, March 2017.
ISSN: 2276-7797 © 2017 Greener Journals
Manuscript Number: 022717028
Effect of Aqueous Leaf Extract of Ocimum Gratissimum on some Haematological Indices in Rats Treated with Zidovudine-containing Antiretroviral Drugs
Ughachukwu PO*1, Okonkwo PO1, Nwozor CM2, Okafor IJ3, Nwafia IN4, Ochiogu BC5
1Department of Pharmacology and Therapeutics,College of Medicine, Chukwuemeka Odumegwu Ojukwu University.
2Department of Human Physiology, College of Medicine, Chukwuemeka Odumegwu Ojukwu University.
3Department of Anatomy,College of Medicine, Chukwuemeka Odumegwu Ojukwu University.
4Department of Medical Microbiology,College of Medicine, Chukwuemeka Odumegwu Ojukwu University.
5Department of Ophthalmology,College of Medicine, Chukwuemeka Odumegwu Ojukwu University.
Background: Anaemia, one of the manifestations of HIV/AIDS, could be worsened by zidovudine-containing antiretroviral therapy. This may result in discontinuation of therapy, drug resistance, and increased mortality and morbidity. The medicinal plant, Ocimum gratissimum, is credited with haematopoetic properties. The study is aimed at finding out if the leaf extract of this plant will protect against anaemia in zidovudine-containing antiretroviral therapy. Methodology: 25 adult rats of both sexes were divided into 5 groups (A, B C, D, E) of 5 animals per group and given oral antiretroviral drugs and extract as follows: A (normal feeds only), B (efavirenz 8.6 mg/kg, abacavir 8.6 mg/kg, zidovudine 4.3 mg/kg), C (extract 80 mg/kg), D (efavirenz 8.6 mg/kg, abacavir 8.6 mg/kg, zidovudine 4.3 mg/kg, plus extract 40 mg/kg), E (efavirenz 8.6 mg/kg, abacavir 8.6 mg/kg, zidovudine 4.3 mg/kg, plus extract 80 mg/kg ) all for 28 days. Thereafter, blood samples were collected and analyzed for haemoglobin and packed cell volume values using autoanalyzer. Data were tested for normality and further analyzed for statistical significance using Kruskal-Wallis test plus Dunn’s multiple comparison using Graph Pad Prism 7.0. Results: Mean values (± S.E.M.) of haemoglobin were 12.7± 0.7, 13.7± 0.8, 11.6 ± 1.4, 12.2 ±1.7, and 13.9 ± 1.0 for groups A, B, C, D, and E respectively. Mean values (± S.E.M.) of Packed Cell Volume (PCV) are 38.2± 2.2, 41.4 ± 2.2, 35.0 ± 4.1, 36.8 ± 4.9, and 41.3 ± 3.0 for groups A, B, C, D, and E respectively. Dunn’s multiple comparison test did not yield any statistically significant differences (P values 0.7368 and 0.7325 for haemoglobin and PCV values respectively). Conclusion: The results show that neither antiretroviral drugs nor extract affected the haemoglobin level or the PCV in the rats during the course of this experiment.
Key words: Antiretroviral drugs, haemoglobin, ocimum gratissimum, rats, zidovudine.
Agarwal D, Chakravarty J, Chaube L, Rai M, Agarwal NR, Sundar S (2010). High incidence of zidovudine induced anaemia in HIV infected patients in eastern India. Indian J Med Res 132: 386-389.
Anonymous. Zidovudine-induced anaemia in HIV/AIDS. Commentary (2010).Indian J Med Res 132: 359-361. Available at: http://medind.nic.in/iby/t10/i10/ibyt10i10p359.pdf (Accessed 1 August 2016).
Curkendall S, Richardson J, Emons M, Fisher A, Everhard F (2007). Incidence of anaemia among HIV-infected patients treated with highly active antiretroviral therapy. HIV Medicine 8: 483–490.
Daka D, Lelissa D, Amsalu A (2013). Prevalence of anaemia before and after the initiation of antiretroviral therapy at ART Centre of Hawassa University Referral Hospital, Hawassa, South Ethiopia. Scholarly Journal of Medicine 3(1): 1-6.
Dierickx D, Kentos A, Delannoy A (2015). The role of rituximab in adults with warm antibody autoimmune hemolytic anemia. Blood 125 (21): 3223-9.
Evans WC (2002). Trease and Evans Pharmacognosy. 15th ed. London: W.B. Saunders; 61.
Kerkhoff AD, Wood R, Cobelens FG, Gupta-Wright A, Bekker L-G, Lawn SD (2014). Resolution of anaemia in a cohort of HIV-infected patients with a high prevalence and incidence of tuberculosis receiving antiretroviral therapy in South Africa. BMC Infect Dis 14: 3860.
Hoff J (2000). Methods of blood collection in the mouse. Lab Animal 29(10): 47-53.
Ikeh C (2013). Protective role of aqueous leaf extract of Vernonia amygdalina del. (asteraceae) and O.cimum gratissimumlinn (lamiaceae) in cyclophosphamide induced urotoxicity and myelosuppression .MSc. Thesis. University of Nigeria, Nsukka. pp.82-85.
Kayode AAA, Kayode OT, Aroyeun OA, Stephen MC 92011). Haematologic and hepatic enzyme alterations associated with acute administration of antiretroviral drugs. J Pharmacol Toxicol 6: 293-302.
Maria GVS, Francisco JAM, Paulo ROL, Fábio OS, Márcio TH (2004). Composition of essential oils from three Ocimum species obtained by steam and microwave distillation and supercritical CO extraction. ARKIVOC 6: 66-71.
Matsumoto T, Endoh K, Kamisango K, Akamatsu K, Koigumi K, Higuchi M, Imai N, Mitsui H, Kawaguchi T (1990). Effect of recombinant human erythropoietin on anticancer drug-induced anaemia. BJH 75(4): 463-468.
National Research Council, NRC (2003). Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research. Washington (DC): Academies Press (US); A Sample Size Determination. Available:http://www.ncbi.nlm.nih.gov/books/NBK43321/. (Accessed 21 August 2015).
Nunez M (2006). Hepatotoxicity of antiretrovirals: Incidence, mechanisms, and management. Journal of Hepatology 44(1): S132-S139.
Ofem O, Ani E, Eno A (2012). Effect of aqueous leaves extract of Ocimum gratissimum on hematological parameters in rats. Int J Appl Basic Med Res 2(1): 38-42.
Pirie NW (1975). Leaf protein; a beneficiary of tribulation. Nature 253: 239-243.
Prabhu KS, Lobo R, Shirwaikar AA, Shirwaikar A (2009). Ocimum gratissimum: A review of its chemical, pharmacological and ethnomedicinal properties. The Open Complementary Medicine 1: 1-15.
Rang HP, Dale MM, Ritter JM, Moore PK (2003). Pharmacology. 5th ed. Philadelphia: Churchill Livingstone.
Rao KV (2014). Drug-induced haematologic disorders. Pharmacotherapy. Available at: http://www.mhpharmacotherapy.com/0071800530/online_pdfs/24_Dipi_Web_Ch24_359-374.pdf (Accessed1 August 1 2016).
Research Animal Resources, RAR (2009). Guidelines for the use of anaesthetics, nalgesics and tranquilizers in laboratory animals. Available at: http://www.ahc.umn.edu/rar/anes thesia.html. (Accessed 20 September 2015).
Renner LA, Dicko F, Koueta F, Malateste K, Gueye RD, Aka E, Eboua T, Azondekon A, Okomo U, Toure P, Ekouevi D, Leroy V(2013). Anaemia and zidovudine-containing antiretroviral therapy in paediatric antiretroviral programmes in the leDEA paediatric West African Database to evaluate AIDS. JIAS 16: 18024.
Shehu A, Umana UE, Timbuak JA, Hamman QW, Musa SA, Ikyembe DT (2012). Effect of Aqueous and Ethanolic Extracts of Ocimum gratissimum on the Histology of the Spleen, Haematological Indicies of Wistar Rats and its Antimicrobial Properties. Asian Journal of Medical Sciences 4(4): 130-133.
Somogyi A, Rosta A, Lang I, Werling K (1996). Treatment of drug-induced bone marrow suppression with recombinant human granulocyte/monocyte colony stimulating factor. Adverse Drug React Toxicol Rev 15(2): 119-124.
Sule OJ, Godwin J, Nnopu IA (2012). Biochemical investigation of hepatotoxic effects of antiretroviral drugs on Wistar albino rats. J Phys Pharm Adv 2(4): 171-175.
Tadele A (2014). Prevalence of Zidovudine induced megaloblastic anemia among HIV/AIDS patients attending University of Gondar Hospital, Northwest Ethiopia. J Blood Disorders Transf 5(8): 143-148.
Ughachukwu PO, Okonkwo PO, Nwozor CM, Okafor IJ, Nwafia I (2016) .Effect of aqueous leaf extract of ocimum gratissimum on antiretroviral drug-induced hepatotoxicity in rats. EJMP 13(1): 1-11.
Uhegbu FO, Elekwa F, Akubugwo EI, Chinyere GC, Iweala EE (2012). Analgesic and hepatoprotective activity of methanolic leaf extract of Ocimum gratissimum. Research Journal of Medicinal Plants 6(1): 108-115.
Ukoha UU, Umeasalugo KE, Dimkpa U, Ndukwe G, Anyabolu AE, Emefo LE (2015). Effects of zidovudine on hemostatic and hematologic parameters in adult rats. J Exp Integr Med 5(2): 85-88.
Youm JW, Wom YS, Jeon JH, Moon KB, Kim HC, Shin KS, Young H, Kim HS (2010). Antibody responses in mice stimulated by various doses of the potato-derived major surface antigen of hepatitis B virus. Clin Vaccine Immunol 17(12): 2029-2032.
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