Umar et al
Greener Journal of Medical Sciences Vol. 7 (2), pp. 018-021, March 2017.
Manuscript Number: 021317025
Molecular Epidemiology of HCV Genotype in Relation to Viral Load of Infected Individuals in Northwestern Nigeria
*1Umar Faruk Abubakar, 2Muhammad Yahaya, 3Sumayya Hamza Maishanu, 2Ismail Ibrahim, 3Aisha Rabiu Ishaq, 4Alo Moses Nnaemeka, 1Abdullahi Sani Ahmad, 5Muhammad Yahaya
1*Public Health and Diagnostic Institute, Northwest University Kano, Nigeria
2Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria
3DNA Labs, Molecular Research and Diagnostic Center Kaduna, Nigeria
4Department of Biological Science, Federal University, Ndufu-Alike, Ebonyi State, Nigeria
5Laboratory Department, Rasheed Shekoni Specialist Hospital, Dutse, Jigawa, Nigeria
The global burden of Hepatitis C Virus (HCV) is increasing and its believed to be one of the leading causes of hepatocellular carcinoma and a major cause of chronic liver disease. The present study aimed at evaluating the prevalence of HCV genotype in association with viral load of infected individuals. A total of one hundred and seventy three (173) HCV seropositive patients were included in the study. RNA extraction was performed using Accuprep RNA extraction kit according to the manufacturer’s instructions.After running the HCV cDNA on 1.5% agarose gel, genotyping was completed using Beckman Coulter sequencing machine based on chain termination method.Accupower HCV-1111 (IVD) was used for the preparation of master mix and light cycler real time PCR machine version 5.2 was used for viral load. The result of the current study revealed that 93 (54%) patients were infected with the viral genotype 1b, followed by 1a with 64 (39%), 1c, 2a and 2b with 2 (1%), 9 (5%) and 5 (3%) respectively. Conclusively, genotype 1b has the highest prevalence, while 1c with lowest number of infected individuals.Moreover, in all the ranges of viral loads in the study, 1b was found to have highest prevalence.
Key Words: HCV, Viral load, Genotype.
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