Umar et al
Greener Journal of Medical Sciences Vol. 7 (2), pp. 018-021, March 2017.
ISSN: 2276-7797 © 2017 Greener Journals
Manuscript Number: 021317025
Molecular Epidemiology of HCV Genotype in Relation to Viral Load of Infected Individuals in Northwestern Nigeria
*1Umar Faruk Abubakar, 2Muhammad Yahaya, 3Sumayya Hamza Maishanu, 2Ismail Ibrahim, 3Aisha Rabiu Ishaq, 4Alo Moses Nnaemeka, 1Abdullahi Sani Ahmad, 5Muhammad Yahaya
1*Public Health and Diagnostic Institute, Northwest University Kano, Nigeria
2Faculty of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria
3DNA Labs, Molecular Research and Diagnostic Center Kaduna, Nigeria
4Department of Biological Science, Federal University, Ndufu-Alike, Ebonyi State, Nigeria
5Laboratory Department, Rasheed Shekoni Specialist Hospital, Dutse, Jigawa, Nigeria
The global burden of Hepatitis C Virus (HCV) is increasing and its believed to be one of the leading causes of hepatocellular carcinoma and a major cause of chronic liver disease. The present study aimed at evaluating the prevalence of HCV genotype in association with viral load of infected individuals. A total of one hundred and seventy three (173) HCV seropositive patients were included in the study. RNA extraction was performed using Accuprep RNA extraction kit according to the manufacturer’s instructions.After running the HCV cDNA on 1.5% agarose gel, genotyping was completed using Beckman Coulter sequencing machine based on chain termination method.Accupower HCV-1111 (IVD) was used for the preparation of master mix and light cycler real time PCR machine version 5.2 was used for viral load. The result of the current study revealed that 93 (54%) patients were infected with the viral genotype 1b, followed by 1a with 64 (39%), 1c, 2a and 2b with 2 (1%), 9 (5%) and 5 (3%) respectively. Conclusively, genotype 1b has the highest prevalence, while 1c with lowest number of infected individuals.Moreover, in all the ranges of viral loads in the study, 1b was found to have highest prevalence.
Key Words: HCV, Viral load, Genotype.
Alter MJ (1999). Hepatitis C virus infection in the United States Journal of Hepatology, 31:88-91.
Anita Chakravarti, GauravDogra, VikasVerma and AmitParkashSrivastava (2011).Distribution pattern of HCV genotypes & its association with viral load.Indian J Med Res 133, 326-331.
Choo QL., Kuo G., Weiner AJ., Overby LR, Bradley DW, Houghton M (1989): Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science, 244(4902):359-362.
Dusheiko G, Schmilovitz-Weiss H, Brown D, McOmish F, 15. Yap PL, Sherlock S ( 2005) Hepatitis C virus genotypes: An investigation of type-specific differences in geographic originand disease. Hepatology; 19: 13-8.
Ederth J, Jern C, Norder H, Magnius L, Alm E, Rognsvag BK, Sundin CG, Brytting M, Esbjornsson J(2016), Mild M infect ecolepidemiol.; 6:306-70.
Esfahani SHZ, Kardi MT, Edalati M (2010).Hepatitis C virus 17. Genotype frequency in Isfahan province of Iran: a descriptive cross-sectional study. Virol J; 7: 69-75.
Houghton M. Hepatitis C viruses. In: Fields BN, Knipe DM, Howley PM (1996), eds. Fields Virology, 3rd ed. Philadelphia, Lippincott - Raven:1035-1058.
Hsu HH, Greenberg HB. Hepatitis C. In: Hoeprich PD, Jordan MC, Ronald AR(1994), eds. Infectious Diseases. A treatise of infectious processes.5th ed. JB Lippincott Co, Philadelphia,:820-825.
Jimenez-Mendez R, Uribe-Salas F, Lopez-Guillen P, Cisneros-Garza L, Castaneda-Hernandez G (2010): Distribution of HCV genotypes and HCV RNA viral load in different regions of Mexico. Ann Hepatol, 9:33–39.
Jing P, Yanju L, Weuyong L, Yaowu Z, Xialing Y, Jingxin X, Xiong W, Yue W, Wie L, ZiyongS, (2015) pLoS One; 10(9): e0137059.
Joseph CF, Jennifer EL, Richard OP, Nallely M, Guo-liang X, David SC, Michael AP, Zoya ED, Dorcas OO, Lili TP, Pavel S, Shirley O, Fred SS, Gilberto V, Hajung R, Ohene KO, Richard SC, Yury EK (2015)pLoS one; 10(12).
Mackay IM. (2004) Real-time PCR in the microbiology laboratory. Clin.Microbiol. Infect. 10:190-212.
Pawlotsky JM (2003): Hepatitis C virus genetic variability: pathogenic and clinical implication. Clin Liver Dis, 7:45–66.
Purcell RH.: Webster RG, Granoff A, (1994)Hepatitis C virusEncyclopedia of Virology. London, Academic Press Ltd, 569-574.
Seeff LB, Hollinger FB, Alter HJ, Wright EC, Cain CM, Buskell ZJ. Long-term mortality and morbidity of transfusion-
associated non-A, non-B, and type C hepatitis: A National Heart, Lung, and Blood Institute collaborative study. Hepatology 2001; 33: 455-63.
Simmonds P, Bukh J, Combet C, Deleage G, Enomoto N, Feinstone S, Halfon P, Inchauspe G, Kuiken C, MaertensG (2005): Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology42(4):962-973.
SulimanQadirAfridi, Muhammad Muddassir Ali., FurqanAwan., MuhammadNaumanZahid., Sara QadirAfridiand TahirYaqubIrfanQadirAfridi (2014). Molecular epidemiology and viral load of HCV indifferent regions of Punjab, Pakistan.Virology journal11-14.
Tsukiyama-Kohara K, Iisuka N, Kohara M, Nomoto A (1992): Internal ribosome entry site within hepatitis C virus RNA. J Virol, 66:1476–1483.
Xie Y, Garza G, Dong J (2016)Hepatitis C virus genotype and subtype distribution in patient specimens tested at the university of Texas medical branch, Galveston, between January 2011 and November 2014 Lab med. 47(2):112-118.