Oguwike et al
Greener Journal of Medical Sciences Vol. 7 (1), pp. 005-011, February 2017.
ISSN: 2276-7797 © 2017 Greener Journals
Manuscript Number: 020617015
Effect of Pawpaw (Carica papaya) Latex on the Haemostatic Mechanism and Biochemical Profile of Male Albino Wistar Rats
Oguwike FN*, Nwafia WC, Nwozor CM, and Okeke JC
Department of Physiology, Chukwuemeka Odumegwu Ojukwu University (Former Ansu) Uli Campus, Anambra State
Extracts of roots, stem, bark, leaves and sap of some medicinal plants have been known to have effect against the most dreaded pathogenic organism like the bacteria, Fungi, viruses (Russel et al, 1997). Soldiers need training, men and women do engage in exercises, children play in the field, most times people are not allowed to partake in their trainings, exercises or play due to bleeding from minor injuries which could be arrested using simple remedies from plants that could be found around the bush. Hence the effect of pawpaw (Carica papaya) latex on the haemostatic mechanism and biochemical profile of male albino wistar rats was studied to show the ability of Carica papaya latex to enhance haemostatic mechanism and stop bleeding.
Forty (40) male albino rats weighing 200-250g were selected for the study. Group 1 (10 rats) is the control group. Group 2 (10 rats) received topical application of the latex after wound laceration, Group 3 (10 rats) was fed orally (Enteral) with the latex for 5 days, while Group 4 (10 rats) was injected (Parenteral) with the latex (0.5ml) for 5 days. Thereafter, blood samples were collected from Groups 1, 3 and 4 for analysis such as Full blood count, Platelet count, Prothrombin time test, Partial thromboplastin time test, bleeding time, clotting time, biochemical test such as serum total bilirubin, conjugated bilirubin, alkaline phosphatise, aspartate transaminase and alanin transaminase.
Calcium and alkaloids (protein precipitates) was discovered present in the latex of Carica papaya. They are known to be involved in the precipitation of coagulation factors (Tafeeq, et al 2008) which promotes clotting in rats. The latex of Carica papaya reduced haemoglobin concentration, packed cell volume and white blood cell count significantly (p<0.05) but the platelet count was very high (p<0.05). It also increased the total bilirubin, conjugated bilirubin and the liver enzymes but shortened bleeding and clotting times in vitro. It could be deduced that the presence of calcium and papain helped to reduce the bleeding and clotting times.
Key words: haemostasis, Carica papaya, clotting time, cuts, haemoglobin, platelets, bilirubin, aspartate transaminase.
Aravid G, Debjit Bhowmik, Duraivel S, Harish G (2013). Traditional and Medicinal uses of Carica papaya. Journal of Medical Plants Studies Year 2013. Vol. 1 Issue 1. www.plantsjournals.com.
Arthur C.G (2014). Haemostasis and blood coagulation. Textbook of Medical Physiology pp 79-80. Pub W>B Saunders company. Philadephia, London Toronto.
Baker F.J (1985). Investigation for haemostatic abnormalities. Investigation to medical Laboratory Technology Butterworths Publication, Worburn London p 348.
Beckstrom S, Stephen M, James A, Wain K.K, (1994). The ethno-botany Database http//:11probe.Nalusada.Gov8300kg:bin//browse/ethnobotdb(ACEDB Version.3dataversion).
Brecher L and Cronkite M (1950). Platelet count. In Dacie J.V ed. Basic Haematological Techniques. Practical haematology 6th Edition. Churchill Livingstone Melbourne p43.
Bush B.M (1991). Interpretation of Laboratory results for small animal clinicians. Oxford, Blackell Scientific publication. P25-34.
Clark R.A.F (1996) ed. The molecular and cellular biology of wound repair 2nd edition, New York Plenum press.
Dejana E, VILLA S, De GACIANO G (1982). Bleeding time in rats. A comparison of different experimental conditions. Thromb. Haemostat 48: 108-111.
Furie B and Furie B.C (1988). The molecular base of blood coagulation. Cell, 53:505-518
Iwu M (1982). African ethno-medicine seminars delivered at institute of Medical Research Yaba Lagos Nigeria P1.
Jean. S. Macalood, Helen J. Vincente, Ranao D, Boniano, Jessie G. Gorospe, Einor C. Roa (2013). Chemical analysis of Carica papaya L, crude latex. American Journal of plant sciences pp 1941-1948.
Kroll M.H (1998). Biochemical basis of platelet activation. Blood 74:1181-1195.
Lee L and White W (1985). Investigation for haemostatic abnormalities. Introduction to Med. Lab Technology, 6th edition Butterworth Publication Woburn p 346.
Lorke D, (1985). A new approval to Practical Acute Toxicity Testing. Arch Toxicol 54: 275-278.
National Research Council, WRC (US) Committee On Guidelines For The Use Of Animals In Neuroscience And Behavioral Research (2003). Guideline for the care and use of Mammals in Neuroscine and Behavioral Research Washington D.C: National Academic Press (US): A sample size Determination Available from: http://www.nchi.nlm.nihi.gov/book/NBK43321//.
Okoli C.O, Akah P.A, Okoli A.S (2007). Potentials of leaves of Aspillia Africa (compositae) in wound care. An experimental evaluation B.M.C complement. Allern Med. 2007:7:24.
Spaet T.H (1964). Mechanism of platelet plug formation and role of adenosine diphosphate. AM. J. Physiol. 206:1267.
Spaet T.H (1966). Studies on the mechanism hereby platelets are clumped by adenosine diphosphate. Thromb. Diath. Haemorrh. 15:36.
Taofeeg O, Oyeniyi M.A, Tesleem A.O (2005). Mechanism of actions of Jatrophagossypifolia stem latex as a hamostatic agent. European journal o General Medicine 2(4): 14-145.
Ureme S.O, Ezimah A.C.U, Akwu C.I (2002). The relationship of clot retraction platelet count and serum calcium concentrations in apparently healthy adolescents in Enugu metropolis. Journal of life an Environmental Science. Vol.4. No.2 p251-254.
Quicks W (1985). Basic hematological techniques; In: Practical haematology, 6th Edition Churchill Livingstone Edinburgh London Melbounre p 43.
Russel B.A, Hardin J.N, Grand L, Traser A (1997). Poissonous plants of North Carolina, Caesalphinasp (Pride of Barbados). Department of Horticulture Science North Caroline state University. http//www.ces.nessy.edu/dept.
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