Greener Journal of Plant Breeding and Crop Science Vol. 3 (1), pp. 015-019, May 2015.
ISSN: 2354-2292 © 2015 Greener Journals
Manuscript Number: 050415065
Mycoflora associated with seeds of five rice (Oryza sativa) varieties in Ghana
Atta Kwesi Aidoo, Zipporah Appiah-Kubi and Ralph K. Bam
CSIR- Crops Research Institute, P.O. Box 3785, Kumasi
Corresponding Author’s E-mail: inceaidoo07@ yahoo. co.uk
An in vitro test was carried out at the Seed Pathology Unit of CSIR-Crops Research Institute to assess the incidence of seed-borne mycoflora associated with seeds of five rice varieties. Two of the varieties namely Jasmine 85 and Toxx 3377 are lowland varieties and the other three, namely Obolo, Nerica 4 and IDSA-85 are upland varieties. The Blotter method was used to identify fungal species. About 31% of the seeds tested were infected by different species of fungi at storage and none of the varieties tested was free from fungal species. Two rice varieties, IDSA 85 and Tox 3377 recorded the highest total fungal infection of 26.7% and 26% respectively while Obolo recorded the least with 9.5%. Sixteen fungal species from 11 genera were identified to be associated with the five rice varieties. The major disease-causing fungi found to be associated with the seeds were: Bipolaris oryzae, Curvularia lunata, Aspergillus flavus, Fusarium moniliforme, F. oxysporum and Rhizopus sp. A. flavus, on Jasmine 85, recorded the highest frequency of occurrence with 21%. It was followed by B. oryzae with frequency of 13.5% on IDSA 85 and C. lunata, 11.5% on Nerica 4. Some of the fungal species showed least frequency of occurrence between 0.5-1%. The most predominant fungus on all the five varieties was C. lunata (25.7%) and was closely followed in order of predominance by A. flavus (23.8%), and B. oryzae (21.2%). Seeds need to be treated before planting to reduce field and postharvest contamination of rice seeds. Development of appropriate and cost-effective technologies to reduce mycoflora infection should be one of the focal points in rice industry in Ghana.
Key words: Rice, mycoflora, seed-borne, fungal infection, predominance.
Butt AR, Yaseen SI and Javaid A (2011). Seed-borne mycoflora of stored rice grains and its chemical control. The Jour. Of Animal & Plant Sciences, 21(2): 2011, pp 193-196.
ISTA (Int. Seed Testing Assoc.) (2001). International Rules for Seed Testing. Rules Amendments. Seed Sci. Technol. 29:1-127.
Javaid MS, Wahid A, Idrees M, Gill MA and Saleem A (2002). Seed mycoflora studies in rice. PakistanJ. Phytopathol. 14: 132-134.
Khan TZ, Gill MA and Khan MG (2000). Seed borne fungi of rice from central Punjab and their control. PakistanJ. Phytopathol. 12:12-14.
Marthur SB and Kongsdal O (2003). Common Laboratory Seed Health Testing Methods for Detecting Fungi, 2nd Edition. International Seed Testing Association. Switzerland.
Moses E, Bam R, Nyarko S and Appiah-Kubi Z (2009). Studies on Rice seed pathogens. CSIR-Crops Research Institute Annual Report for 2009, Ghana. 60pp.
Neergaard P (1986). Seed Pathology. S. Chand & Company Ltd. New Delhi.
Nguefack J, Nguikwie SK and Fotio D (2007). Fungicidal potential of essential oils and fractions from Cymbopogon citratus, Ocimum gratissimmum and Thymus vulgaris to control Alternaria padwickii and Bipolaris oryzae, two seed-borne fungi of rice (Oryza sativa L.). J. Essential Oil Res.17: 581-587.
Nutsugah SK (1997). Survey of rice diseases/pests in northern Ghana. Proc. of the 6th Annual WARDA Integrated Pest Mgt.-Task force meeting, March2-5, 1997, Bouake, Cote d’Ivoire.
Wikipedia (2015). List of rice diseases. en.wikipedia.org (Accessed on 26/03/2015).
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