Noman and Kadi
Greener Journal of Physical Sciences Vol. 2 (1), pp. 020-026, January, 2012.
ISSN: 2276-7851 © 2012 Greener Journals
Manuscript Number: GJPS1205
High Performance Liquid Chromatography Assay with UV Detection for the Determination of Ciprofloxacin in Human Plasma
*Maged Alwan Noman1and Hussein O.kadi 2
1Faculty of Pharmacy, Department of Pharmaceutics, Sana'a University, Yemen,
2Department of pharmacology, Faculty of Medicine, Sana'a University, Yemen,
*Corresponding Author Email: magedalwan65 @ gmail.com
sensitive and specific isocratic High Performance Liquid
Chromatography method with UV detection was developed,
validated and applied for the determination of Ciprofloxacin
in human plasma. Sample preparation was carried out with the
addition of an internal standard Gatifluoxacin to plasma
samples followed by a liquid-liquid extraction using
perchloric acid. Separation was achieved with Waters
symmetry C18, 5µm, 150 x 3.9 mm column using a mixture of
potassium phosphate buffer (pH =3.1) : acetonitrile :
methanol (72:12:16, v:v:v) in addition to
tetrabeutyleammonium hydroxide as an ion pair reagent. The
calibration curve for Ciprofloxacin in plasma was linear in
the range 0.5-10 µg/mL with r2 = 0.9998. The lower limit of
quantitation (LLOQ) and limit of detection (LOD) for
Ciprofloxacin in plasma were respectively 0.032 µg/mL and
Inter and intra-day precessions in the measurements of three different levels QC samples in human plasma were in the range 0.85-2.47%, 0.14-0.24% and 0.10-0.35% respectively.
Method recovery, short-and long term stabilities were within the acceptable values. The developed method of analysis provided a sensitive and specific assay for Ciprofloxacin and was found to be suitable for the analysis of the drug in human plasma.
Keywords: Ciprofloxacin , HPLC, human plasma.