Omodara et al
Greener Journal of Physical Sciences Vol. 3 (4), pp. 159-164, May 2013.
ISSN: 2276-7851 © 2011 Greener Journals
Manuscript Number: 011713391
Infrared and Ultraviolet Spectroscopic Analysis of Methanol Extract of Phyllanthus Muellerianus Root
Omodara N.B., Amoko J.S, Obijole O.A and Ojo B.M
Department of Chemistry Adeyemi College of Education Ondo
*Correspondence Author’s Email: basiniyi @ yahoo. com
The roots sample of phyllanthus muellerianus was extracted with Hexane and Methanol respectively, given the percentage yield of 2.37% and 10.18% respectively. Column chromatography of the Methanol extract afforded 3 crystals which were labeled as M, N, Q. IR and The UV analysis shows that M gave absorption peaks at 238nm and 326nm, Q gave absorption peaks at 240nm and 326nm while N gave peaks at 220nm and 240nm. The IR analysis shows absorption for M at 1378-1 and Q at 1378.5cm-1, also, there are other absorption band for M, N and Q at 1465.9cm-1, 1475.6cm-1 and 1470.9cm-1 respectively which suggest C-H bending of saturation. It was also shown that there are absorption of M,N and Q at 1704.3cm-1, 1699.4cm-1 and 1699.4cm-1 respectively. Other absorption for M and Q at 1704.-1 and 1699.4cm-1 which suggest C-H sretching and absorption at 2939.8-1 and 2934.8cm-1 which indicate the aldehyde functional group. Furthermore, absorptions were shown for M, N and Q at 3446.5cm-1, 3440.7c-1 and 3438.2cm-1 respectively which suggest O-H stretching.
Keywords: column chromatography, extract, crystal, absorption peaks