Greener Journal of Science, Engineering and Technology Research

Open Access


Subscribe to 

our monthly News letters

Impact Calculation

Edem et al

Greener Journal of Science, Engineering and Technological Research Vol. 5 (1), pp. 011-019, May 2015.

ISSN: 2276-7835 © 2015 Greener Journals

Research Paper

Manuscript Number: 061715081



Effects of Novel Substrates in electricity generation in a mediator-less microbial fuel cell


Edem, D.E., Opara, C.C., Evbuomwan, B.O. and *Oforkansi, B.C. 

Department of Chemical Engineering, University of Port Harcourt, Nigeria.

*Corresponding Author’s E-mail:  benjaminoforkansi @


The use of Microbial Fuel Cells to produce electricity is an area that has attracted attention recently owing to clean and renewable energy produced by the use of bacteria to catalyze the conversion of organic matter into electricity. The experiment was done in a mediator-less Microbial Fuel Cell with cassava mother liquid, human urine, human urine with faeces, swine waste and brewery waste as substrates using potassium ferricyanide as electron acceptor. The result of the experiment indicates that cassava mother liquid, human urine and swine waste generated a maximum potential difference of 1.32V each and also maximum current of 2.28A,2.19A and 2.02A respectively. However, human urine with faeces and brewery waste was observed to be lower with values 1.13V and 1.15V respectively and corresponding lower currents of 1.45A and 1.40A respectively. It was observed that human urine is most stable with potential difference and current of 1.32V and 1.58A respectively. This may be attributable to the rich organic compounds such as carbohydrate, nitrogen, ammonium, potassium and phosphorous in urine which are good sources of fuel.


Keywords: Microbial fuel cell, Electricity generation, mediatorless.


View/get involved, click [Post Review]


Aelterman P,Rabaey K, Hai The pham,Boon N and Verstraete W (2006).Continous electricity generation at high voltages and currents using stacked microbial fuel cells. Environ. Sci. Tech. 40: 3388-3394.


Azunna  O, Opara CC and Oji A (2014).Generation of Bio-Electricity from Ammonia Fertilizer plant liquid effluent, Greener J. of Bioch. and Bio tech. 1(1):001-017.


Habermann W and  Pommer E (1991).Biological fuel cells with sulphide storage capacity. Appl. Microbiol. Biotechnology. 35: 128-133.


Kassongo J and Togo CA (2011). Evaluation of fuel strength paper mill effluent for electricity generation in mediator-less microbial fuel cells. African J. of Biotech. 10 (69):15564-15570.


Logan BE and Regan JM (2006). Electricity producing bacterial communities in microbial fuel cells. Trends in Microbiology 14 (12): 512-518.


Mathuriya AS and Sharma VN (2009). Bioelectricity production from various wastewaters through microbial fuel cell technology. J. Biochem. Tech. 2:133-137.


Mokhtarian N,Rahimnejad M,Najafpour GD,Wan RWD and Ghoreyshi AA (2012).Effects of different substrate on performance of microbial fuel cell, African J. of Biotech. 11(14):3363-3369.


Momoh OLY and Neayor B (2010). Generation of electricity from Abattoir waste water with the aid of a relatively cheap source of catholyte. J.Appl. Sc. Environ Manage. 14 (2): 21-27.


Nwahia CR and Opara CC (2014). Study of Microbes Immobilized Monolithic Electrodes in Microbial Fuel Cell, Greener J. of Biochem.  and Biotech. 1(2) : 035-051.


Rabaey K and  Verstraete W (2005).Microbial fuel cells: novel biotechnology for energy generation. Trends Biotech. 23:291-298.


Rabaey K, Boon N, Siciliano S, Verhaege M and Verstraete W (2004).Biofuel cells select for microbial consortia that self-mediate electron transfer. Appl. Environ. Microbiology 70:5373-5382.


Zhen H, Shelley DM and Largus TA (2005). Electricity Generation from Artificial wastewater using an upflow Microbial Fuel Cell. Environ. Sc. Techno. 39 (14) :5262-5267.