Greener Journal of Soil Science and Plant Nutrition Vol. 2 (1), pp. 001-009, April 2015.
ISSN: 2354-2292 © 2015 Authors
Manuscript Number: 021615032
Effect of Moringa Oleifera Extracts on the Growth, Yield and Nutrient Uptake of Spinach (Spinacia Oleracea L.)
Abdel–Rahaman Mohamed Amin Merwad
Soil Science Department, Faculty of Agriculture, Zagazig University, 44511 Zagazig, Egypt
Corresponding Author’s Email: abdo.soil @ yahoo.com, Tel: +2 01212144240
A field experiment was carried out during 2014 growing season to study the effect of foliar sprayed with the aqueous and ethanolic Moringa oleifera leaf extracts at rates of 0, 1, 2, 3 and 4 % on growth, yield and nutrients uptake ( N, P, K, Ca, Mg and Fe) of spinach plants (Spinacia Oleracea L.) Results showed that spinach plants treated with four concentrations of aqueous and ethanolic Moringa extracts (1, 2, 3 and 4%) showed significant increase in plant height, leaf area, fresh and dry weight, chlorophyll a, chlorophyll b, carotenoids, ascorbic acid, phenols and nutrients uptake (N, P, K, Ca, Mg and Fe) compared to those untreated control plants. Spraying the ethanolic moringa extract at different rates show favorable effects on growth parameters, yield, photosynthetic pigments and nutrients uptake as compared to the aqueous extract. The application of 1% to 4% of aqueous and ethanolic Moringa extracts gave increase of fresh weight which ranged from 126 - 457 % and 237 – 620%, respectively. The study recommends the application of both extracts at 4%. Thus, it is concluded that Moringa oleifera leaf extract can be recommended to be used effectively by famers as a bio-organic fertilizer for various crops due to its high productivity, high nutritive value, antioxidant effect, easy preparation, low cost and environmentally friendly nature.
Key words: Moringa oleifera leaf extract, Photosynthetic pigments, nutrients uptake, spinach plants.
Abdalla M M and El-Khosiban N(2012). The palliative effect of bio-organic fertilizer on lead pollution in Lycopersicum esculentum plants. J. Basic App.Sci. 8:1-12
Abdalla M A (2013). The potential of Moringa oleifera extract as a biostimulant in enhancing the growth, biochemical and hormonal contents in rocket (Eruca versicaria subsp. Sativa) plants. Int. J . Plant Physiol. Biochem. 5(3): pp. 42-49.
Anwar F , Latif S, Ashraf M and Gilani A H (2007). Moringa oleifera: A food plant with multiple medicinal uses. Phytother. Res. 21:17- 25.
AOAC (1984). Official Method of Analysis
Balakumbahan R and Rajamani K (2010). Effects of biostimulants on growth and yield of Senna ( Cassia angustifolia var KKM1). J. Hort. Sci. Ornam Plants 2(1):16-18.
Basra SMA, Zahoor R., Rehman H , Afzal I and Farooq M (2009 b). Response of root applied brassica and moringa leaf water extracts on seeding growth in sunflower. In : Proceedings of the International Conference on Sustainable Food Grain Production: Challenges and Opportunities. University of Agriculture, Faisalabad, Pakistan, pp.23.
Basra SMA, Zahar M , Rehman H , Yassmin A and Munir H. (2009a). Evaluating the response of sorghum and moringa leaf water extracts on seeding growth in hybrid maize. In: Proceedings of the International Conference on Sustainable Food Grain Production: Challenges and Opportunities. University of Agriculture, Faisalabad, Pakistan, pp.22.
Black C A (1968). "Soil plant relationships". 2nd Ed. John Wiley and Sons, of the Association of Official Analytical Chemists, 14th ed, Published by the Association of Official Analytical Chemists, PO Box, 540, Benjamin Franklin Station, WashingMg, dc.20044.
Chapman DH and Pratt PF (1961). "Methods of analysis for soil, plant and waters" California. Univ.; Division of Agric. Sci.
Culver M , Tagwira F and. Chiteka AZ (2012). Effect of moringa extract on growth and yield of tomato. Greener J. Agric. Sci. 2(5):207-211.
Culver M, Tagwira F and Chiteka AZ (2013). Effect of moringa extract on growth and yield of maize and common Beans. Greener J. Agric. Sci.3(1):55-62.
El Awady A (2003). Moringa Tree: Nature’s Pharmacy. http://www.islamonline.net/english/Science/2003/02article06.shtml(download 20/10/2007).
Epstein E and Bloom A J (2005). Mineral Nutrition of Plants: Principles and Perspectives. 2 nd ed. Sinauer Associates , Sunderland, MA,USA.
Foidl N , Makkar HPS and Becker K (2001). The potential of Moringa oleifera for agricultural and industrial uses. In: Proceedings of International Workshop"What development potential for Moringa product" Dares –Salaam, Tanzania. pp.47 – 67.
Fuglie LJ (2000). The Miracle Tree : Moringa oleifera: Natural Nutrition for the Tropics. The multiple Attributes of Moringa, pp.172.
Hasegawa PM , Bressan RA , Zhu JK and Bohnert HJ (2000). Plant cellular and molecular responses to high salinity. Annu.Rev. Plant Physiol. 51: 463- 499.
Jackson M L(1958). "Soil Chemical Analysis''.Prentice Hall, Ic., Englewood califfs, New Jersy.
Jacob SJP and Shenbagaraman S (2011). Evaluation OF antioxidant and antimicrobial activities of the selected green leafy vegetables. Int. J. Pharm. Tech.Res. 3(1):148 – 152.
Jyostsna V and Srivastava AK (1998). Physiological basis of salt stress resistance in pigeonpea (Cajanus cajan L.). II. Pre-sowing seed soaking treatment in regulating early seeding metabolism during seed germination. Plant Physiol. Biochem. 25 : 89- 94.
Makkar HPS and Becker K (1996). Nutritional value and antinutritional components of whole and ethanol extracted of Moringa oleifera leaves. Anim. Feed Sci. Technol. 63:211-228.
Metzner H , Rau H and Senger H (1965).Unter suchungen zur synchronisier barkeit ein zeiner pigment. Mangol Mutanten von Chlorella plants. 65: 186-191.
Moyo B, Masika PJ, Hugo A and Muchenje V(2011). Nutritional characterization of Moringa (Moringa oleifera Lam) leaves. Afr. J. Biotechnol.10(60):12925 – 12933.
Mvumi C, Tagwria F and Chiteka AZ (2012). Effect of Moringa Extract on growth and yield of Tomato .Greener J. Agric.Sci. 2(5): pp.207-211.
Mvumi C , Tagwria F and Chiteka AZ (2013). Effect of Moringa Extract on growth and yield of Maize and common Beans .Greener J. Agric.Sci. 3(1): pp.55-62.
Nicholas L (1978). The function and metabolism of ascorbic acid in plants. Ann. Bot.1996:661 – 669.
Noori JM , Ardalan M M, Nezami MT, Cherati A and Alizadeh G (2010). Effect of organic fertilizers (compost Amol, vermicompost, animal manure) and manganese on chlorophyll concentrations and some of nutrient concentrations of soybean (Glycine max L). OFIS Conferences, Islamic Azad Univ of Bonjord Karaj Branch.pp.18-27.
Nouman W, Siddiqui, MT and Basra SMA(2011). Moringa oleifera leaf extract : An innovative priming tool for rangeland grass. Turk. J. Agric. For. TUBITAK 35: 1- 11.
Owusu D (2008). Phytochemical composition of Ipomea batatus and Moringa oleifera leaves and crackers from Underutilized Flours. MSc. Thesis. Dept of Biochem and Biotech, Faculty of BioScience, College of Science , Kwame Nkrumah University of Science Technology.
Phiri C (2010). Influence of Moringa oleifera leaf extracts on germination and early seedling development of major cereals. Agric. Bio. J.N Amer.1(5):774-777.
Piper CS (1950). "Soil and Plant Anaylsis". Interscince Publishers Inc. New York.
Prabhu M, Kumar AR and Rajamani K (2010). Influence of different organic substances on growth and herb yield of sacred basil ( Ocimum sanctum L) Ind. J. Agric. Res.44(1): 1409 – 1414.
Rajanandh, M.G. and Kavitha, J. (2010). Quantitative estimation of β-sitosterol, total phenolic and flavonoid compounds in the leaves of Moringa oleifera. Int. J. Pharm. Tech. Res.2(2):1409-1414.
Roe JH and Keuther CA (1953). The determination of ascorbic acid in whole blood urine through 2,4-nitrophenyl hydrazine derivative of dehydro –ascrobic acid. J. Biol. Chem.147: 399- 407.
Russell DF (1991). MSTAT C, Director of crop and soil science dept. Michigan State Univ. USA Version 2.10.
Schuphan W (2005). Nutritional value crops as influenced by organic and inorganic fertilizer treatments. Plant Foods Hum. Nuttr.23(4):333-358.
Siddhuraju P and Becker K (2003). Antioxidant properties of various solvent extracts of total phenolic constituents from three different agro-climatic origins of drumstick tree (Moringa oleifera lam).J. Agric.Food Chem.15:2144-2155.
Simons, T.S. and Ross, A.F. (1971). Changes to phenol metabolism associated with system resistance to tobacco mosaic virus in Samsum NN tobacco. Phytopath. 61:1261- 1268.
Sivakumar V and Ponnuami V (2011). Influence of spacing and organic on plant nutrient uptake of Solanum nigrum. Plant Arch. 11(1):431- 434.
Talreja T (2011). Biochemical estimation of tree primary metabolites from medicinally important plant Moringa oleifera Int. J. Pharma. Sci. Rev. Res.7(2):186-188.
Watanabe FS and Olsen SR(1965). Test of ascorbic acid method for determing phosphorus in water and NaHCO3 extracts from soil. Soil Sci. Soc. Am. Proc., 29 : 677 – 678.
Yameogo CW, Bengaly MD, Savadogo A, Nikiema, PA and Traore SA(2011). Determination of chemical composition and nutritional values of Moringa oleifera leaves. Pak. J. Nutr. 10(3):264-268.
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