Greener Journal of Agricultural Sciences Vol. 7 (7), pp. 160-166, September 2017.

ISSN: 2276-7770 

Research Article

Manuscript Number: 090117117



Haematological and Serum Biochemical
Indices of Growing Cockerels Fed Varied Levels of Azolla-Turmeric Mixture


Alagbe J. O.


Farms Limited, Ibadan


This study was conducted to examine the haematological
and some serum biochemical indices of growing Isa cockerels fed diets
supplemented with Azolla- turmeric mixture. One hundred and twenty (120) four
weeks old Isa white cockerels were divided into four groups with three
replicates, each of ten birds in a completely randomized design. Treatment 1
was fed basal diet without Azolla – turmeric mixture (ATMP), treatment 2, 3 and
4 were fed basal diets supplemented with ATMP at levels 5, 10 and 15% respectively.
Proximate analysis shows that Azolla powder contained 22.51% crude protein,
3.53% ether extracts, 14.13% ash, 18.15% crude fibre and 37.88% nitrogen free
extract while turmeric powder contained 9.14% crude protein, 2.40% ether
extracts, 8.69% ash, 4.10% crude fibre and 69.10% nitrogen free extracts. The
basal diet was formulated to meet the nutritional requirements of birds
according to NRC (1994).

All the hematological parameters evaluated : Pack cell
volume (PCV), Haemoglobin (Hb), White blood cell (WBC), Red blood cell (RBC),
Mean corpuscular haemoglobin concentration (MCHC), Mean corpuscular volume
(MCV), Mean corpuscular haemoglobin (MCH) were not significantly(P>0.05)
affected by the inclusion of ATMP. However, there were no significant (P>0.05)
differences among all treatment groups in the values of total protein, albumin,
globulin, alkaline phosphatase, uric acid, creatinine, SGPT and SGOT throughout
the experiment which lasted for 90days. It was concluded that Azolla –turmeric
mixture could be added up to 15% in the diet growing cockerels without any
adverse effects on their blood profile.


Keywords: growing cockerels, haematological parameters,
biochemical indices, Azolla-turmeric mixture.

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Agarwal M, Walia S, Dhingra S,
Khambay BPS (2001) Insect growth inhibition, antifeedant and antifungal
activity of compounds isolated /derived from Zingiber officinale Roscoe (ginger) rhizomes. Pest Management
Science 57:289-300.


Adeyemo, G. O., Ologhobo, A.D and
Adebiyi, O. A (2010) The effect of graded levels of dietary methionine on the
heamatology and serum biochemistry of broilers. Int. Journal of Poult. Sci
9(2): 158-161.


Azzez, O. I., Oyagbemi, A. A and
Oyewale, J. O (2009) Diurnal fluctuation in heamatological parameters of
domestic fowl in the hot humid tropics. Int. Journal of Poult. Sci 8(3):


Alagbe, J.O (2017). Growth
performance and blood parameters of weaner pigs fed diets supplemented with
turmeric powder (TUP). Scholarly Journal of Agricultural Science Vol 7(2),
Pages 57-61. March, 2017. Available online at ISSN 2276-7118 © 2017 Scholarly-Journals


Ajao, B. H., Ola, S. I., Adameji, O.
V., Kolawole, R. F (2013) The relationship of ambient temperature and relative
humidity to the thermorespiratory function of greater grass cutter. Proc. of
the 18th Annual Conf. Animal Sci. Assoc. of Nig 92.


Adenkola, A. Y and Durotoye, L.A
(2004) Haematological study during pre-partum periods in brown savannah does in
Zaria, Nig. Proc. of 38th Annual Conf. of the Agric Soc. Of Nig. 538-540.


Ameen, S. A., Adedeji, O. S.,
Akingbade, A. A., Olayeni, T. B., Ojedapo, L. O and Aderinola, A (2007) The
effects of different regimes on haematology parameters and immune status of
commercial broilers in derived savannah zone of Nigeria. Proc. of 32nd
Annual Conf. Nig. Soc. Of Ani. Prod. 146-148.


Alagbe, J.O (2016). Effect of
feeding varying levels of tiger nuts (Cyperus esculentus) seed meal on the
performance and blood profile of Weaner grass cutters. Scholarly Journal of
Agricultural Science Vol 7(1), Pages 15-19. Feb, 2017. Available online at


Alalade, O.A and Iyayi, E. A (2006).
Chemical composition and the feeding value of Azolla meal for egg type chicks.
Int’l Jou. Of Poult. Sci. 5(2): 137-141.


Abdi-Hachesoo, B., Talebi, A and S.
Asri-Rezaei (2011) Comparative study on blood profiles of Indigenous and
Ross-308 Broiler breeders. Global Vet., 7:238-241


Campbell,  J. R and Lasley , J. F (1975) The science of
animal that serve man. 2nd edn McGraw Hill Book Company New York,
USA 200-202.


Church, J. P., Judd, J. T., Young,
C.W., Kebay,  J. L and Kim, W. W (1984).
Relationship among diet constituents and specific serum clinical components of
subjects eating self selected diets. Afr. Jour. Clin. Nutr. 40:1338-1344.


Chattopadhyay, I., Biswas, K.,
Bandyopadhyay, U and Banerjee, R.K (2004) Turmeric and Curcumin: Biological
actions and medicinal applications. Curr Sci. 87:44-53.


Dein, F. J (1984) Laboratory manual
of avian heamatology. Association of Avian Veterinarian , East North Port, USA.


Daramola, J. O., Adeloye, A. A.,
Fatoba, T. A and Soladoye, A. O (2005) Haematological and biochemical
parameters of WAD goats. Livestock Res. For Rural Dev. 17(8) Available at: http://www.irrd.org17/8/clara/17095.htm.


Etim, N. N., Enyenihi, G. E.,
Akpabio, U and Offiong, E. E (2014a) Effects of nutrition on the heamatology of
rabbits: A review. European Scientific Journal, 10(3):413-424


Ekenyem (2001) Response of broiler
chicks to diets supplemented with garlic essential oil as natural growth
promoter. Int. Journal of Science and Research Vol3 Issue 5 May 2001.


Swenson, M. J (1970) Physiologic
properties, cellular and chemical constituents of blood. In: Dukes’ Physiology
of Domestic Animals, 8th Edition Comstock publishing Associates,
Cornell University Press, Ithaca and London, UK. Pp 21-26


Olorede, B. R and Longe, O. G (2002)
Effect of replacing palm kernel cake with sheabutter cake on egg quality and serum
chemistry of laying hens. Nig. Journal. Prod. 27: 19 – 23.


Olabanji, R. O., Farinu, G. O.,
Akinlade, J. A and Ojebiyi, O. O (2007) Growth performance and heamatological
characteristics of weaner rabbits fed cyanide in processed cassava peel meals
on heamatological and biochemical indices of growing rabbits. Proc. of the 35th
Annual Conf. Nigeria Society of Anim. Prod. 212.


Iyayi, E. A (1994). Supplemental
effect of low and high cyanide cassava on the performance, nutrient
digestibility and serum metabolites of growing pigs. J. Agric. Trop. Sub-trop


Ikepeama, N., Tayyem, R.F and Rock,
C.L (2014). Curcumin content of turmeric and curry powders. Nutr.Cancer.55(2)


NRC, National Research Council
(1994) Nutrients Requirements of Poultry. (9th rev. Ed). National
Academy Press. Washington D.C USA.


Huda-faujan., Noriham, A.,
Norrakiah, A and Babji, A (2009). Antioxidants activity in plants methanoic
extracts containing phenolic compounds. African Journal of Biotechnology 8(3):


Isaac, O.S (2013). Haematological
parameters and factors affecting their values, Published by Science and

Education Centre of North America.
Agricultural science Volume 2, Issue 1 (2014), 37-47. ISSN 2291-4471.


Kececi, T and R. Col (2011)
Haematological and biochemical values of the blood of pheasants of different
ages. Turkish Journal of Veterinary and Animal Science. 35:149-156.


Kamal, M.E Youssef., Amira M. El
Newihi., Soad M. Omar and Zeinab S. Ahmed (2014) Assessment of proximate
chemical composition, nutritional status and antioxidants of curcumin and
mustard seed powder. Food and Public Health 2014, 4(6): 286-292.


Duncan, D.B. (1955). Multiple Range
and Multiple F-Test Biometrics 11:1-42                              


Ibrahim Albokhadaim (2012).
Haematological and some biochemical values of indigenous chickens in Al-Ahsa,
Saudi Arabia during summer season. Asian Journal of Poultry Sciences.
6(4):138-145, 2012.


Daramola, J. O., Adeloye, A. A.,
Fatoba, T. A and Soladoye, A. O (2005) Heamatological and serum biochemical
parameters of WAD goats. Livestock. Res. For Rural Dvp. 17(8)


Etim NseAbasi N., Glory E Enyenihi.,
Uduak, Akpabio and Edem, E. A Effiong (2007). Heamatological parameters
indicators of the physiological state of farm animals. British Journal of Science
10(1): 33-45.


Bolu, S.A.O., Sola-Ojo, F.E .,
Olorunsanya, O.A and Idris, K (2009) Effect of graded levels of dried pawpaw
seed on the performance, heamatology , serum biochemistry and carcass
evaluation of broiler chicken. Int. Journal of Poultry Sci. 8(9):905-909,


Talebi, A., Asri-Rezaei, S.,
Rozeh-Chai, R and Sahraei, R (2005). Comparative studies on haematological
values of broiler strains ( Ross, Cobb, Arbo-acres and Arian). Int’l Journal of
Poultry Sci., 4:573-579.


Togun, V. A., Oseni, B.S. A.,
Ogundipe, J.A., Arewa, T. R., Hammed, A. A., Ajonijebu, D.C., Oyeniran, A.,
Nwosisi, I and Mustapha, F (2007) Effects of Chronic Lead Administration on the
Haematological Parameters of Rabbits- A Preliminary Study. Proc of the 41st
Conf. of the Agric. Soc. Of Nig., 341


Ritchie, B.W., J. G. Harrison and
R.L. Harrison (1994) Avian Medicine, Wingers Publishing Inc. Florida.


Reitman, S and Frankel, S (1957)
Method for serum analysis using the colorimetric SGOT/SGPT assay. Animal
Journal of Clinical Pathology.


SAS Institute (1994) SAS / STAT
users guide. Version 6.1, 3rd Ed. SAS Inst., Cary, N.C


Scott, H.M (1965) Measurement of the
amino acid content of fish meal protein by chick growth assay. Poult. Sci Pp:


Saleh, J. L., Njidda, A. A.,
Adeniji, A.A and Lawan, G. B (2014) Haematological and Biochemical Indices of
Rabbits fed Graded Levels of Browse Forage in Semi Arid Environment. Global
Journals Inc (2014).


Wikivet. Haematology ; 2013.
Available at: www.enwikipedia/wiki/haematology


Tyras Rini Saraswati., Wasmen
Manalu., Damiana Rita Ekastuti and Nastiti Kusumorini (2013) Increased egg
production of Japanese quail by improving liver function through turmeric
powder supplementation. International Journal of Poultry Science 12(10):
601-614 (2013).


Kurtoglu, F., Kurtoglu, V., Celik,
I., Kececi, I and Nizamlioglu, M (2005) Effect of dietary boron supplementation
on some biochemical parameters, peripheral blood lymphocytes, splenic plasma
cells and bone characteristics of broiler chicks given diets with adequate or
inadequate cholecalciferol content. Br. Poultry Sci., 46:87-96.


Onyeanusi, L.  (2007). Hematological and Serological
Characteristics of Cockerels Fed Different Levels of Tiger nut seed meal.
International Journal of Multidisciplinary Research and Review .Vol. 1 (1):


Mangala, H.S and Mathew, G.R (1986)
Direct feed microbials and their direct impact on the intestinal micro flora
and immune system of chickens. Journal of Poultry Science 47:106-114.


Yanagita, K.., Shiraishi, J.,
Kawakami, S and Bungo, T (2011) Time course changes in the blood parameters and
expression of diencephalic CRH and AVT mRNA due to acute isolation stress in
chicks.  J.  Poult. Sci 48(2): 125-129.

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