Rocha Et Al

Rocha et al



Greener
Journal of  Agricultural Sciences


Vol. 4 (4), pp.
110-116,
May 2014.


 ISSN: 2276-7770 



Research

Paper

Manuscript Number: 012314055

DOI: http://dx.doi.org/10.15580/GJAS.2014.4.012314055

 



Introduction of the neMDH gene in Urochloa brizantha
for Aluminium Tolerance in Agronomic Assays

 



Flavio Rocha1,
Keila Maria Roncato Duarte*2,



Luiz Humberto Gomes1, Waldssimiller Teixeira
Mattos3,



Simone Possedente Lira1, Paulo Bardauil
Alcantara3

 


1
Laboratório
de Química Orgânica de Produtos Naturais,


Departamento de Ciências Exatas, Escola Superior de
Agricultura “Luiz de Queiroz”, USP, Piracicaba,
13418-900, 
São Paulo, Brazil.


2
CPD
Genetic and Animal Reproduction, Institute of Animal
Science and Pastures, APTA/SAA Nova Odessa, 13460-000,
São Paulo, Brazil.


3
CPD
Pastures and Nutrition , Institute of Animal Science and
Pastures,


APTA/SAA Nova Odessa, 13460-000, São Paulo, Brazil.

 


*Corresponding Author
’s


Email: keiladuarte @ globo. com

Abstract:


Agricultural production in acid soils constituting about
half of the total arable land on Earth, is limited by
aluminium toxicity, which is manifested by root growth
reduction, decreasing nutrients and water absorption by the
plant. This work evaluated aluminium resistance in genotypes
of Brachiaria brizantha cv. MG4, transformed with the
neMDH gene, by using a nutrient solution for agronomic
evaluation and gene integration confirmation by PCR. Three
from the five confirmed transformed genotypes of B.
brizantha
cv. MG4 with the neMDH gene presented
tolerance to aluminium solutions

(111 µmol L
-1
and 444 µmol L -1).
Two from those three genotypes
 also
showed higher dry weight production in comparison to the
wild-type genotype.

Keywords:
Brachiaria, Tolerance, Aluminum, Soil, Transgenic, neMDH.

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