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Greener Journal of Plant Breeding and Crop Science Vol. 6 (2), pp. 007-014, May 2018.
ISSN: 2354-2292 © 2018 Greener Journals
Research Articles
Manuscript Number: 042918065
(DOI: http://doi.org/10.15580/GJPBCS.2018.2.042918065)
An Optimized Inoculation Method of Fusarium Wilt (Fusarium oxysporum f. Sp. cubense) Causal Agent of Banana Wilt Disease in Ethiopia
Endriyas Gabrekiristos, Mohammed Yesuf and
Getachew Ayana
Ethiopian Institute of Agricultural Research, Melkassa Agricultural Research Center P.O. BOX 436, Adama, Ethiopia.
Abstract
Banana is among the most important fruit crops in
Ethiopia and is produced widely by small scale farmers. Despite its importance
and cultivation, its production is, however, hampered by different biotic and
abiotic factors. Fusarium wilt of banana caused by soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc) is becoming an important production constraint of the crop. In
Ethiopia it was reported in 2014 in potential banana producing areas mainly in
Dale, Aleta Chuko and Samen Bench in Southern Nations, Nationalities and
Peoples Regional State. The general management option to this disease is
replacing susceptible cultivars by resistant/tolerant ones. Use of
resistant/tolerant varieties is the most cost-effective and sustainable method
of managing the disease. A reliable and rapid screening method is needed to
select resistant/tolerant banana varieties. In Ethiopia there is no developed
protocol to artificially inoculate banana fusarium wilt in order to screen
resistant/tolerant variety. An optimized inoculation methods by root dipping
into an adjusted suspension of Foc
inoculum concentration 1x106 conidia/ml on acclimatized three
months old tissue cultured plantlets of susceptible variety (poyo) has enabled to observe typical symptom of FW
by tenth days of inoculation and an overall
result was obtained within three months. This protocol facilitates the
way to produce symptoms of fusarium wilt in banana and screen
resistant/tolerant variety released in Ethiopian Research system. Therefore,
this paper presents the details of the protocol and achievements of the study
in major banana growing areas of Ethiopia
Keywords: Banana, Fusarium
wilt, fusarium oxysporum f. Sp. Cubense, Inoculation method
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References
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