Greener Journal of Microbiology and Antimicrobials

Greener Journal of Microbiology and Antimicrobials Vol. 2 (3), pp. 032-048, July 2014.

 ISSN: 2354-2284 

Review Paper

Manuscript Number: 0521014241

 

 

Mycotoxins in Stored Agricultural Products: Implications to Food Safety and Health and Prospects of Plant-derived Pesticides as Novel Approach to their Management

 

1*Enyiukwu D.N., 1Awurum A.N., 2Nwaneri J.A.

 

1Department of Plant Health Management, Michael Okpara University of Agriculture Umudike, PMB 7269 Umuahia abia state Nigeria. Tel. +234 (0)803 744 689.

2Minor Roots Crops Programme, National Roots Crops Research Institute Umudike, PMB 7009 Umuahia, Abia State.

 

*Corresponding Author’s Email: enyidave2003 @gmail .com

Abstract:

Mycotoxins are toxic secondary metabolites from some species of Aspergillus, Fusarium Penicillium and their related fungi. Their contamination results generally in inferior quality of produce and produce end-products, food safety and ultimately barriers trans-border marketability of agro-produce. Though the presence of mycotoxigenic fungus on produce does not always translate to presence of mycotoxins; however, mycotoxins are silent killers and their effects could be submerged over a long period of time especially during intermittent exposures. These fungal toxins when ingested or inhaled participate in several metabolic dysfunctions in human and livestock systems such as immunological imbalance, cancers, allergies, abortions and in some cases death of the affected hosts. Conditions of high temperatures and relative humidity coupled with poor store standards and hygiene which characterized tropical subsistence farmsteads encourage mouldiness and mycotoxin contamination of agricultural products. The mycotoxins of most agricultural importance (aflatoxins, fumonisins, ochratoxins, trichothecenes) were enumerated in this review while their management strategies were also highlighted; with emphasis on plant-derived anti-mycotoxigenic interventions useable in low-input agricultural production systems of sub-Saharan Africa.

Keywords: Mycotoxins, plant-derived fungicides, Aspergillus spp., Fusarium spp., Penicillium spp., trichothecenes.

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Reference:

Adebayo GB, Otunola GA, Ajao TA (2010). Physico-chemical, microbiological and sensory characteristics of kunu prepared from millet, maize and guinea corn and stored at selected temperature. Adv. J. Food. Sci. Tech. 2(1): 41-46.

Adjou ES,  Dahouenon-Ahoussi E,  Degnon R,  Soumanou MM,  Sohounhloue DCK (2012). Investigations on the bioactivity of essential oil of Ageratum conyzoides L. from Benin against the growth of fungi and aflatoxin production     . Int. J. Pharm. Sci. Rev. Res. 13(2): 143-148.

Aghili SR, Khosravi AR, Shokohi T, Salmanian B. (2009). Survey of contamination mycoflora of consumed rice in Manzadaran, Iran. www.icef11.org/content/.../fms1247.pdf

Aja-Nwachukwu J,  Emejuaiwe SO (2006). Aflatoxin-producing fungi associated with Nigerian maize. Environmental Toxicology and Water Quality; 9(1): 17-23.

Allameh A,  Ziglari T,  Rasooli I (2011). Phytoinhibition of growth and aflatoxin biosynthesis in toxigenic fungi. Aflatoxins – detection, measurement and control. Torres-Pacheco (Ed). In Tech Croatia, Pp 283-316.

Amadioha AC (2012). Reducing food losses through sustainable methods of plant disease management: an imperative for actualization of food security in Nigeria. A paper presented at the 13^th inaugural lecture MOUAU, June, 2012.

Amra H (2007). Natural occurrence of some mycotoxins in local and imported corn grains (Zea mays L.) in Egypt. Toxicol Lett. (Suppl. 1): S205.

Anokwuru CP,  Ezekiel CN,  Alabi AO,  Shallie APD,  Haruna MT (2011). Ameliorative effects of ethanolic neem extracts on dietary aflatoxin induced haematological damage and hepatoxicity in mice. Asian J. Med. Sci. 3(6): 254-260.

Arya A (2010). Recent advances in the management of plant pathogens: botanicals in the fungal pest management. In: Management of fungal plant pathogens. Arya A,  Perello AE (Eds). UK, CAB International, pp. 1-25.

Asadi M, Solaeymarinad J, Kazemi A, Tabrizchi H. (2005). Histopathologic findings induced by using contaminated foods with mycotoxins. www.medlib.ir/.../histopathologic-fingings-ind...

Awurum AN, Enyiukwu DN (2013). Evaluation of the seed-dressing potentials of phytochemicals from \Carica papaya and Piper guineense on the germination of cowpea (Vigna unguiculata L. Walp) seeds and incidence of the seed-borne fungi. Continental Journal of Agricultural Science 7(1): 29-35.

Awurum AN,  Ucheagwu PO. (2013). Effects of duration of contact of seed extract of Piper guineense, Monodora myristica and Xylopia aethiopica on the germination and incidence of seed-borne fungi of stored cowpea (Vigna unguiculata L. Walp) seeds. Continental J. Biol. Sciences 6(1): 37-42.

Awurum AN, Okwu DE,  Onyekoro PO (2005). .In vitro and in vivo control of Colletotrichum destructivum in cowpea using some plant extracts. J. Sci., Tech and Envt.; 5(1-2): 52 – 57.

Ayalew A  Fehrmann H  Lepschy J  Beck R  Abate D (2006). Natural occurrence of mycotoxins in staple cereals from Ethiopia. Mycopathologia 162: 57-63.

Ayidin A  Gunsen U  Demirel S (2008). Total aflatoxin, aflatoxin B1 and Ochratoxin A levels in Tukish wheat flour. J. Food Drug Anal. 16: 48-53

Bandyopadhyay R,  Kumar M,  Leslie JF (2007). Food Additives and Contaminants 24 (10): 1109-1114.

Bankole SA,  Adebanjo A (2003). Mycotoxins in food in West Africa: current situation and possibilities of controlling it. African Journal of Biotechnology 2(9): 254-263.

Bankole SA Mabekoje OO (2004). Occurrence of aflatoxins and fumonisins in Preharvest maize from Southwestern Nigeria. Food Addit. Contam. 21: 251-255.

Bankole SA Ogunsanwo BM Mabekoje OO (2004). Natural occurrence of moulds and aflatoxins B1 in melon seeds from markets in Nigeria. Food Chem. Toxicol. 42: 1309-1314.

Bankole SA, Ogunsanwo BM, Eseigbe DA. (2005). Aflatoxins in Nigerian dry-roasted groundnuts. Food Chem. 89(4): 503-506.

Banu N,  Johnpaul M (2010). Taxol as chemical detoxificant of aflatoxin produced by Aspergillus flavus isolated from sunflower seed. Health 2(7): 789-795.

Bennett JW,  Klich M (2003). Mycotoxins. Clin. MicroBio Rev. 16: 497-516.

Bhat RV (2008). Human health problems associated with current agricultural food production. Asian Pac. J. Clin. Nutr. 17(5); 91-94.

Bhat RV, Vasanthi S  (2003).  Mycotoxin food safety risks in developing countries: Food safety in food security and food trade. 2020 Vision for Food and the Environment; Focus 10, Brief 3 of 17 September, 2003.  

Binder EM,  Tan LM,  Chin LJ,  Handl J,  Richard J (2007).  Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients.  Animal Feed Sci. Tech. 137: 265-282.

Bryden WL (2007). Mycotoxins in the food chain: human health implications. Asian Pac. J. Clin. Nutr. 16(1): 93-101.

Dalie DKD,  Deschamps AM,  Richard-Forget F (2010). Lactic acid bacteria – potential for control of mould growth and mycotoxins: a review.  Food Control. 21(4): 370-380.

Da Costa CL, Geraldo MRF, Arroteia CC, Kemmelmeier C (2010). In vitro activity of neem oil on Aspergillus flavus growth, sporulation viability of spores, morphology and aflatoxin B₁ and B₂. Advances in Biosciences and Biotechnology 1: 292-299.

Doko MB,  Rapior S , Visconti A,  Schjoth JE. (1995). Incidence and levels of fumonisin contamination in maize genotypes grown in Europe and Africa. J. Agric. Food Chem. 43(2): 429-434.

El-Nagerabi SAF,  El-shafie AE,  Alkhanjari SS,  Al-Bahry SN,  Elamin MR (2013). The potential of Baobab (Adansonia digitata L.) extracts as biocontrol on the growth and aflatoxin production by Aspergillus flavus and A. parasiticus. J. food Res. 2(3): 93-103.

Elbert D,.  Von Czapiewski,  K,.  Bujara,  I,.  Kunze, J,.  Giger, A. (2010). Simultaneous analysis of 10 mycotoxins in crude extracts of different types of grains by LC-MS/MS. AB SCIEX, Damstadt, Germany GmbH, Hamburg, Germany, Publication NOs 1121410-01, Pp 1-6.

EMAN (European Mycotoxin Awareness Network) (2013). Long-term mycotoxin analysis highlights the need for careful mycotoxin risk management. EMAN Summer Newsletter 2013.pdf. www.leatherhead foods.com

Enyiukwu DN,  Awurum AN. (2013). Fungitoxic principles and antifungal activity of extracts from Carica papaya and Piper guineense on Colletotrichum destructivum. Continental J. Biol. Sci. 6(1): 29-36.

Enyiukwu DN, Awurum AN,   Nwaneri JA (2014a). Efficacy of plant-derived pesticides in the control of myco-induced postharvest and storage rots of tubers and agricultural products: A review. Net Journal of Agricultural Sciences; 2(1): 30-46.

Enyiukwu DN,  Awurum AN, Ononju CC,  Nwaneri JA (2014b). Significance of characterization of secondary metabolites from higher plants in plant disease management. International Journal of Advanced Agricultural Research. 2: 8-28.

Etop S (2013). Importance and control of aflatoxins in food industry. www.aflasafe.com/../get_file?p.

Ezekiel CN,  Kayode KO,  Fapohunda SO,  Olorunfemi MF,  Barinaada TK (2012). Aflatoxic moulds and aflatoxins in street-vended snacks in Lagos Nigeria. Internet J. Food Safety; 14: 83-88. 

FAO (Food and Agricultural Organization of the United Nation) (2003). Mycotoxins in grains. www.fao.org/wawdocs/ic5008e01.htm   Retrieved December 28, 2013.

FAO (Food and Agricultural Organization of the United Nation) (2004). Worldwide regulations for mycotoxins in food and feed in 2003. FAO Food and Nutrition Paper No 81. Rome: FAO, PP 1-180.

Fapohunda SO (2014). Moulds, meals, man and mycotoxins. Nigeria Mycotoxin Awareness and Study Network NMASN.    

Fapohunda SO (2013). Impact of mycotoxins on sub-Saharan Africa: Nigeria as a case study. European Mycotoxins Awareness Network. www.mycotoxins.com Retrieved Wednesday 19, 2014.

Farag DME   (2008).  Aflatoxins: Awareness and control. Dubai International Food Safety Conference 24-27 February, 2008, Pp 1-55.

Farombi EO (2003). African Indigenous plants with chemotherapeutic potentials and biotechnological approach to production of bioactive prophylactic agents. Afr. J. Biotech. 2(12): 662-671.

Goswami RS, Dong Y, Punja ZK. (2008). Host range and mycotoxin production by Fusarium equiseti isolates originating from ginseng. Can. J. Plant Pathol. 30: 155-160.

Gurjar MS, Ali S, Akhtar M, Singh KM (2012). Efficacy of plant extracts in plant disease management. Agricultural Sciences 3(3): 425 – 433.

Herman T, Trigo-Stockli D (2002). Mycotoxins in feed grains and ingredients. Department of Grain Science, Kansas State University  (KSU) www.oznet.ksu.edu

Houssou PA, Ahuhuendo BC, Jacobsem M, (2009). Natural infection of cowpea by toxigenic fungi and mycotoxins in Benin West Africa. J. Stored Prod. Res. 45(1): 40-44.

Hussain, A,.   Shafqatullah,  J,.  Ali, Z. (2012). Inhibition of aflatoxin producing fungus using chemical, herbal compounds/spices and plants. Pure Appl. Bio. 1(1): 08-13.    

Ijei, II,.  Obidoa, O.  (2010).  Biochemical and histological changes in liver of albino rats fed diets incorporated with Vernonia amygdalina and V. colorata leaves. Int. J. Med. and Med. Sci. 2(10): 285-289.

Iowa State University (2014). Mycotxins: Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University. 2014

John P,  Steve E (2010). Mycotoxin contamination of corn – what it is, what it does to pigs and what can be done about it. Iowa state University Extension. IPIC 12: 1-7.

Kana HA,  Aliyu IA,  Chammang HB (2012). Review on neglected and under-utilized root and tuber crops as food security in achieving the millennium development goals in Nigeria. J. Agric. Vet. Sci. 4: 27-33.

Kazemi A. (2008). Survey of consumed rice contamination to mycotoxigenic fungi. Med. J. Tabriz Univ. Med. Sci. 30(3): 111-118.

Kazemi A. (2011). Genomic projects of mycotoxigenic fungi: A review. Proceedings of the International Symposium on mycotoxins in nuts and dried fruits, Damghan Iran, Acta Horticulturae 963, September 10-12, 2011.

Kazemi A. (2013). Fungal genomic projects, metagenomics and gene analysis. Jundishapur J. Microbiol. (Special edition) 14-15.

Kazemi A. (2013a). Fungal contamination of flours in bakeries in Tabriz city, Iran. www.sid.ir/.../viewpaper.asp?...

Kazemi A, Ostadrahimi A, Ashrafnejad F, Sargheini N, Mahdavi R, Farshchian M. (2014). Mold contamination of untreated and roasted with salt nuts (walnuts, peanuts and pistachios) sold at markets in Tabriz Iran. Jundishapur J. Microbiol. 7(1): 1-5.

Kazemi A, Nia JM, Rezaeian F, Milani FZ, Jabarri M, Maghami SJG, Mahdavi R, Rahimi ARO. (2009). Fungal contamination of consumed tea in East Azarbaidjan Province of Iran. J. Med. Plants 8(29): 147-155.

Kishore KG,  Pande S,  Manjula K,  Narayana R,  Thomas D (2002). Occurrence of mycotoxins and toxigenic fungi in groundnut (Arachis hypogaea L.) seeds in Andhra Pradesh, India. Plant Pathol. J. 18(4): 204-209.

Kritzinger Q, Aveling TAS, Marasas WFO, Rheeder JP, Van Der Westhuizen L, Shephard G. (2003). Mycoflora and fumonisin mycotoxins associated with cowpea (Vigna unguiculata L. Walp.) seeds. J. Agric. Food Chem. 51; 2188-2192.

Lawley R (2013). Aflatoxins. Food Safety Watch www.foodsafetywatch.org/facts Retrieved March 22, 2014.

Leggott NL, Shephard GS. (2001). Palutin in South Africa Commercial apple products. Food Control. 12:73-76.

Lino CM Silva LJG Pena A Fernandez M Manes J. (2007). Occurrence of fumonisins B1 and B2 in broa, typical Portuguese maize bread. Int. J. Food Microbiol. 118: 79-82.

Li FQ, Luo XY, Yoshizawa T. (1999). Mycotoxins (trichothecenes, zearalenone and fuminosins) in cereals associated with human red-mould intoxications stored since 1989 and 1991 in China. Nat. Toxins 7: 9-97.

Lotlikar PD,  Raj HG,  Bohm LS,  Ho LL,  Jhee E,  Tsuji K,  Gopalan P (1989). A mechanism of AFB1-DNA binding in the liver by Phenobarbital pre-treatment of rats. Cancer Research; 49: 951-957.

Lutfullah G, Hussain A. (2012). Studies on contamination level of aflatoxins in some cereals and beans of Pakistan. Food control 23(1): 32-36.

Okello DK,  Kaaya AN,  Bisikwa J,  Were M,.  Olota  HK (2010). Management of aflatoxins in groundnuts. Manual for farmers, proceessors, traders and consumers in Uganda. National Agricultural Research Organisation/Makerere University, 27pp.

Okigbo RN (2004). A review of biological control methods for postharvest yams (Dioscorea spp.) in storage in South Eastern Nigeria. KMITL Science Technology Journal 4(1): 207-215.

Okigbo, R. N. and Nmeka, I. A. (2005). Control of yam tuber rot with leaf extracts of Xylopia aethiopica and zingiber officinale. African journal of Biotechnology 4(8): 804-807.

Onuegbu BA (1999). Evaluation of the efficacy of fungicides, plant extracts and chemicals in minimizing mould growth in mung bean (Vigna radiata L.) seeds. Legume Research 22(4): 270-272.

Ostadrahimi A, Ashrafnejad F, Kazemi A, Sargheini N, Mahdavi R, Farshchian M, Mahluji. (2014). Aflatoxin in raw and salt-roasted nuts (Pistachios, peanuts and walnuts) sold in markets of Tabriz, Iran. Jundishapur J. Microbiol. 7(1): 1-5 DOI: 10.5812/jjm.8751

Miller JD (1999). Fungi and mycotoxins in grain: implications for stored products research. Proceedings of the 6^th international Working Conference on Stored Product Research Vol. 2, PP 971-977.

Muthoni JW  NdungoJK Gathumbi Jk, Mutitu EW, Wagacha JW. (2008). The occurrence of fusarium species and mycotoxins in Kenyan wheat. Crop Protec. 27: 1215-1219.

Murphy PA,  Hendrich S,  Langren C,  Bryant CM (2006). Food mycotoxins: an update. J. Food Sci. 71(5): 51-65.

Mycotoxin Information (2013). Mode of action, toxicology and metabolism of mycotoxins. Scientist Veterinarian www.mycotoxins.info – Scientist / Veterinarian – Mo… Retrieved September 20, 2013

Nwinyi OC,  Abikoye BA (2010). Antifungal effects of pawpaw seeds extracts and papain on postharvest Carica papaya L. fruit rot. African J. Agric. Res. 5(12): 1531-1535.

Peraica M,.  Radic B,.  Lucic A,.  Pavlovic M.  (1999).  Toxic effects of mycotoxins in humans. Bulletin of the World Health Organisation (WHO); 77(9):754-766.

Papachan KS,  Prassad R,  Vijay VK,  Strivastava AK (2007). Evaluation of antibacterial activity of six Indian spices against common food-borne pathogens. Int. J. Food Sci. Tech. 42: 910-915.

Paterson RRM. (2007). Aflatoxins contamination in chilli samples from Pakistan. Food Control 18: 817-820.

Razzaghi-Abyaneh, M,.  Shams-Ghahfarokhi, M,.  Peng-Kuang, C.  (2010). Aflatoxins: Mechanism of inhibition by antagonistic plants and bacteria.  …(Eds) www.Intechopen.com/books/...586-304.

Reddy KRN,  Salleh B,  Saab B,  Abbas HK,  Abel CA,  Shier WT  (2010a.) An overview of mycotoxin contamination of foods and its implication for human health. Toxin Review; 29(1): 3-26. 

Reddy KRN,  Raghavender CR,  Reddy BN,  Shalleh B (2010b). Biological control of Aspergillus flavus growth and subsequent aflatoxin B₁ production in sorghum grains. Afr. J. Biotech. 9(27): 4247-4250.

Reddy VK,  Srinivas M,  Reddy AR,.  Srujana, G,.  Surenkha,  Reddy, SM. (2011). Plant extracts in the management of aflatoxin production by Aspergillus flavus. Int. J. Phama. Bio. Sci. 2(2): 492-498.

Reddy CR,  Reddy AR,  Waliyar F,  Alur AS,  Gowda CLL, Reddy BVS,  Rao PP (2013). Mycotoxins in sorghum and pearl millet: effect on human and animal health and their management. CFC/FAO Paper Pp 1.

Saxena N Premendra DD, Kausar MA, Das M. (2008). Patulin in apple juices: Incidence and likely intake in an Indian population. Food Addit. Contam. 1(2): 140-146.

Sorrenti S,  Di Giacomo C,  Aquivivq R,  Barbagallo  J,  Bognanno M,  Galrano F (2013). Toxicity of Ochratoxin A and its modulations by antioxidants: a review. Toxins 5: 1742-1766.

Tagne, A,. Feujio, T. P,. and Sonna, C. (20086). Essential oils and plant extracts as potential substitutes to synthetic fungicides in the control of fungi. Endure International Conference, Diversifying Crop Protection, France, October 12-15, 2008, pp 1-3..

Tiffany, I. (2013). The implication of aflatoxin contamination for local food safety in Senegal. www.hungercenter.wpengine.netdna-cdn.com/

Thanaboripat, D. (2011). Control of aflatoxins in agricultural products using plant extracts. KMITL Sci. Tech. J. 11(1): 35-42.

Tripathi P,  Dubey AK. (2004). Exploitation of natural plant products as an alternative strategy for control of postharvest fungal rotting of fruits and vegetables. .Postharvest Biol. Tech. 32(3): 235-245.

Viljoen A (2004). Status and prevalence of mycotoxins. Department of Plant Pathology, Stellenbosch University. www.afma.co.za/.../9.%20%20Mr%20Dr...

Wagacha JM, Mutomi JW (2008). Mycotoxin problem in Africa: current status, implication to food safety and health and possible management strategies. Int. J. Food Microbiol. 124(1): 1-12.

Whitlow LW,  Hagler WM  (2013).  Mycotoxin contamination of feedstuffs – an additional stress factor for Diary cattle, 32pp. www.cals.ncsu.edu/.../myco~1.pd...  Retrieved November 10, 2013.

World Health Organization (WHO) 2006. Mycotoxins in African foods: Implications to food safety and health. AFRO Newsletter WHO Food Safety (FOS) Issue 2, July 2006.

Yoshinari T,  Akiyama T,  Nakamira K,  Kuodo T,  Takahashi Y,  Muraoke Y,  Nonumura Y,  Nagasawa H,  Sakuda S (2007). Diostatin A is a strong inhibitor of aflatoxin production by Aspergillus parasiticus. Microbiology 153: 2774-2780.