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Umedum et al

Greener Journal of Microbiology and Antimicrobials

Vol. 5(1), pp. 06-15, 2020

ISSN: 2354-2284

Copyright ©2020, the copyright of this article is retained by the author(s)

https://gjournals.org/GJMA

 

 

 

 

 

 

 

Pathogenicity Profiles of Aspergillus Species Isolated from Phaseolus vulgaris (Bean) Seeds

 

 

Umedum, C.U1.; Iheukwumere, C.M.2; Iheukwumere, I.H.1*

 

 

1 Department of Microbiology, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria

2 Department of Applied Microbiology& Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria 

 

 

ARTICLE INFO

ABSTRACT

 

Article No.: 043020064

Type: Research

 

 

The role of Aspergillus Species in cases of food borne infection have been established and reported to have substantial degree of morbidity and mortality among immunoincompetent individuals. This study was carried out to determine the pathogenicity of Aspergillus species isolated from bean seed samples. A total of 90 representative bean seed samples of different varieties were randomly collected from different shops and open markets in Ihiala Local Government Area, Anambra State, and screened for the presence of Aspergillus species. The isolates obtained were characterized and identified using macroscopic, microscopic and molecular characteristics. The pathogenic potentials of the isolates on immunocompetent and immunoincompetent mice were investigated by oral administration of 0.5 ml of the inoculum (108cells/ml) into the mice. The mice were kept under complete observation for 4 weeks for obvious pathological features and mortalities. It was observed that 32 (35.56 %) and 14 (14.44 %) of the studied bean seed samples recorded Aspergillus species for surface and internal contaminants. Oloka bean seeds (70 %/30 %) were most contaminated. Aspergillus flavus strain HUS6 (AFHUS6), Aspergillus niger strain HG48 (ANHG48), Aspergillus niger strain HUS1 (ANHUS1), Aspergillus tubingiensis strains EM-CN1 (ATEM-CN1), Aspergillus aculeatus strain AN5 (AAAN5) and Aspergillus awamori strain DN-SN2 (AW DN-SN2) were isolated from the bean seed samples, of which AN HUS1 was mostly seen in the studied samples There were significant (P<0.05) obvious pathological features and lesion on the internal organs, majorly the lungs of the infected immunoincompetent mice, and the pathological features of those mice infected with AFHUS6 and ANHUS1 were most pronounced. There were significant (P<0.05) mean plate counts of the test isolates from the organs of immunoincompetent mice, and decreased in organ weight to body weight ratio. Thus, this study has shown that the fungi isolates showed obvious pathological features majorly on infected immunoincompetent mice, of which AFHUS6 was most pathogenic.

 

Accepted:  05/04/2020

Published: 28/06/2020

 

*Corresponding Author

Iheukwumere, I. H.

E-mail: ikpower2007@ yahoo.com

 

Keywords:

Aspergillus Species; mycotoxin-producing; fungi pathogenicity; Phaseolus vulgaris

 

 


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Cite this Article: Umedum, CU; Iheukwumere, CM; Iheukwumere, IH (2020). Pathogenicity Profiles of Aspergillus Species Isolated from Phaseolus vulgaris (Bean) Seeds. Greener Journal of Microbiology and Antimicrobials, 5(1): 6-15.

 

 


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